The histochemical antibody for 5-HT1A receptor is generated in a rabbit against synthetic peptide sequence corresponding to amino acids 294-312 of the rat 5-HT1A receptor. The antiserum is provided as 100 µl of affinity purified serum containing 1% BSA. Controls: The ImmunoStar 5-HT1A receptor antiserum was quality control tested using standard immunohistochemical methods.
The antiserum demonstrates strongly positive labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/200 - 1/300 in PBS - Bn/Av-HRP detection. Intensification methods such as nickel will approximately double the dilution factor as recommended.
The antibody was characterized by immunohistochemistry and Western blot. Western blot showed a single band of approximately 45 kD using a dilution of 1/100. The western information is included as specificity information. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography.
BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT1A receptors.
Photo Description: IHC image of neurons staining for the 5-HT1A receptor in the rat cortex (above) and 5HT1A receptor staining of astrocytes in the rat hippocampus (below). The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:500 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
Availability: In Stock
Reacts With: Guinea Pig, Human, Monkey, Mouse, Rat
Alternate Names: 5-hydroxytryptamine receptor 1A; ADRB2RL1; ADRBRL1; RAT5HT1A, 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled, anti-5-HT 1A
Immunogen: Rat 5-HT1A receptor (294-312)
Gene Symbol / ID, Accession #: Htr1a, 24473
|PROD #||ANTIBODY||CITATION||SPECIES REACTIVITY||APPL METHOD||RESEARCH AREA||# CITED BY||PUBMED ID|
|24504||5-HT (Serotonin) 1A Receptor Antibody||J Comp Neurol. 2008 Aug 10;509(5):474-92. doi: 10.1002/cne.21770. Bone morphogenetic protein regulation of enteric neuronal phenotypic diversity: relationship to timing of cell cycle exit. Chalazonitis A1, Pham TD, Li Z, Roman D, Guha U, Gomes W, Kan L, Kessler JA, Gershon MD.||Mouse, Rat||IHC||Cell Cycle||18537141|
|24504||5-HT (Serotonin) 1A Receptor Antibody||Vasudeva, Rani K., and Barry D. Waterhouse. "Cellular Profile of the Dorsal Raphe Lateral Wing Sub-region: Relationship to the Lateral Dorsal Tegmental Nucleus." Journal of chemical neuroanatomy (2014).||IHC||0||24704911|
|24504||5-HT (Serotonin) 1A Receptor Antibody||Ahn, Seong-Ki, and Carey D. Balaban. "Distribution of 5-HT and 5-HT receptors in the inner ear." Brain research 1346 (2010): 92-101.||Rat, Monkey||IHC||8||20510890|
|24504||5-HT (Serotonin) 1A Receptor Antibody||Hadjighassem, Mahmoud R., et al. "Human Freud-2/CC2D1B: a novel repressor of postsynaptic serotonin-1A receptor expression." Biological psychiatry 66.3 (2009): 214-222.||Human||IHC||19||19423080|
|24504||5-HT (Serotonin) 1A Receptor Antibody||Wang, Guo-Du, et al. "Mast cell expression of the serotonin1A receptor in guinea pig and human intestine." American Journal of Physiology-Gastrointestinal and Liver Physiology 304.10 (2013): G855-G863.||Human, Guinea Pig||WB||2||23518679|
|24504||5-HT (Serotonin) 1A Receptor Antibody||Vantrease, Jaime E., et al. "5-HT1A Receptors of the Nucleus Tractus Solitarii Facilitate Sympathetic Recovery Following Hypotensive Hemorrhage in Rats." American Journal of Physiology-Heart and Circulatory Physiology (2015): ajpheart-00117.||Rat||0||25980022|
|24504||5-HT (Serotonin) 1A Receptor Antibody||Lee, Choong Hyun, Ji Hyeon Ahn, and Moo-Ho Won. "New expression of 5-HT1A receptor in astrocytes in the gerbil hippocampal CA1 region following transient global cerebral ischemia." Neurological Sciences 36.3 (2015): 383-389.||Gerbil||1||25253632|
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This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.Download MSDS