5-HT (Serotonin) 1A Receptor Antibody

5-HT (Serotonin) 1A Receptor Antibody
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Price: $375.00
Product ID : 24504

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Description

The histochemical antibody for 5-HT1A receptor is generated in a rabbit against synthetic peptide sequence corresponding to amino acids 294-312 of the rat 5-HT1A receptor. The antiserum is provided as 100 µl of affinity purified serum containing 1% BSA. Controls: The ImmunoStar 5-HT1A receptor antiserum was quality control tested using standard immunohistochemical methods.

The antiserum demonstrates strongly positive labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/200 - 1/300 in PBS - Bn/Av-HRP detection. Intensification methods such as nickel will approximately double the dilution factor as recommended.

The antibody was characterized by immunohistochemistry and Western blot. Western blot showed a single band of approximately 45 kD using a dilution of 1/100. The western information is included as specificity information. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography.

BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT1A receptors.

Photo Description: IHC image of neurons staining for the 5-HT1A receptor in the rat cortex (above) and 5HT1A receptor staining of astrocytes in the rat hippocampus (below). The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:500 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Hippocampus

Host: Rabbit

Quantity / Volume: 100 µL

State: Liquid

Availability:  In Stock

Reacts With: Guinea Pig, Human, Monkey, Mouse, Rat

Alternate Names:  5-hydroxytryptamine receptor 1A; ADRB2RL1; ADRBRL1; RAT5HT1A, 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled, anti-5-HT 1A

RRID: AB_572210

Immunogen: Rat 5-HT1A receptor (294-312)

Gene Symbol / ID, Accession #:  Htr1a, 24473



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Publications

PROD # ANTIBODY CITATION SPECIES REACTIVITY APPL METHOD RESEARCH AREA # CITED BY PUBMED ID
24504 5-HT (Serotonin) 1A Receptor Antibody J Comp Neurol. 2008 Aug 10;509(5):474-92. doi: 10.1002/cne.21770. Bone morphogenetic protein regulation of enteric neuronal phenotypic diversity: relationship to timing of cell cycle exit. Chalazonitis A1, Pham TD, Li Z, Roman D, Guha U, Gomes W, Kan L, Kessler JA, Gershon MD. Mouse, Rat IHC Cell Cycle 18537141
24504 5-HT (Serotonin) 1A Receptor Antibody Vasudeva, Rani K., and Barry D. Waterhouse. "Cellular Profile of the Dorsal Raphe Lateral Wing Sub-region: Relationship to the Lateral Dorsal Tegmental Nucleus." Journal of chemical neuroanatomy (2014). IHC 0 24704911
24504 5-HT (Serotonin) 1A Receptor Antibody Ahn, Seong-Ki, and Carey D. Balaban. "Distribution of 5-HT and 5-HT receptors in the inner ear." Brain research 1346 (2010): 92-101. Rat, Monkey IHC 8 20510890
24504 5-HT (Serotonin) 1A Receptor Antibody Hadjighassem, Mahmoud R., et al. "Human Freud-2/CC2D1B: a novel repressor of postsynaptic serotonin-1A receptor expression." Biological psychiatry 66.3 (2009): 214-222. Human IHC 19 19423080
24504 5-HT (Serotonin) 1A Receptor Antibody Wang, Guo-Du, et al. "Mast cell expression of the serotonin1A receptor in guinea pig and human intestine." American Journal of Physiology-Gastrointestinal and Liver Physiology 304.10 (2013): G855-G863. Human, Guinea Pig WB 2 23518679
24504 5-HT (Serotonin) 1A Receptor Antibody Vantrease, Jaime E., et al. "5-HT1A Receptors of the Nucleus Tractus Solitarii Facilitate Sympathetic Recovery Following Hypotensive Hemorrhage in Rats." American Journal of Physiology-Heart and Circulatory Physiology (2015): ajpheart-00117. Rat 0 25980022
24504 5-HT (Serotonin) 1A Receptor Antibody Lee, Choong Hyun, Ji Hyeon Ahn, and Moo-Ho Won. "New expression of 5-HT1A receptor in astrocytes in the gerbil hippocampal CA1 region following transient global cerebral ischemia." Neurological Sciences 36.3 (2015): 383-389. Gerbil 1 25253632

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MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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