5-HIAA (5-Hydroxyindoleacetic Acid) Antibody

5-HIAA (5-Hydroxyindoleacetic Acid) Antibody
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Price: $250.00
Product ID : 24274

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Description

The antibody produces moderate labeling of raphe neurons in normal rat. In rats whose serotonergic system has been activated, staining intensity is increased to a maximum label. Recommended dilution of the antiserum is 1/4000-1/8000 for biotin-streptavidin/HRP technique.

The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Schipper and Tilders, 1983). Results showed that the 5-HIAA antibody dose dependently stained 5-HIAA but did not stain any concentration of 5-HT or 5-HTP. The antiserum was also tested by pre-adsorption at 25 µg/mL with various BSA conjugates. While pre-adsorption with 5-HIAA conjugate completely eliminates immunolabeling, pre-adsorption with conjugates of 5-HT,5-HTP and dopamine had no effect on staining intensity or distribution of stain.

Photo Description: IHC image of neurons staining for 5-H1AA in the raphe nucleus of the rat brainstem. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. 


Host: Rabbit

Quantity/Volume: 100 µL

State: Lyophilized Whole Serum

Reacts With: : Rat, Tritonia Diomedea (Sea Slug)

Alternate Names:
anti-5-HIAA

RRID: AB_572208

Immunogen: 5-HIAA

Gene Symbol: SLC6A4

Entrez Gene ID: 6532

NCBI Gene Aliases: 5-HTT,5-HTTLPR,5HTT,HTT,OCD1,SERT,hSER

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Publications

PROD # ANTIBODY CITATION SPECIES REACTIVITY APPL METHOD RESEARCH AREA # CITED BY PUBMED ID
24274 5-HIAA (5-Hydroxyindoleacetic Acid) Antibody Appel, NATHAN M., JOSEPH F. Contrera, and ERROL B. De Souza. "Fenfluramine selectively and differentially decreases the density of serotonergic nerve terminals in rat brain: evidence from immunocytochemical studies." Journal of Pharmacology and Experimental Therapeutics 249.3 (1989): 928-943. Rat 99 2732954
24274 5-HIAA (5-Hydroxyindoleacetic Acid) Antibody J Neurochem. 2005 Dec;95(5):1495-503. Epub 2005 Oct 7. In vivo interaction between serotonin and galanin receptors types 1 and 2 in the dorsal raphe: implication for limbic seizures. Mazarati AM1, Baldwin RA, Shinmei S, Sankar R. Rat, Mouse IF 16219029
24274 5-HIAA (5-Hydroxyindoleacetic Acid) Antibody Fickbohm, David J., Nadja Spitzer, and Paul S. Katz. "Pharmacological manipulation of serotonin levels in the nervous system of the opisthobranch mollusc Tritonia diomedea." The Biological Bulletin 209.1 (2005): 67-74. Tritonia Diomedea (Sea Slug) IHC 7 16110094

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MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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