The antibody produces moderate labeling of raphe neurons in normal rat. In rats whose serotonergic system has been activated, staining intensity is increased to a maximum label. Recommended dilution of the antiserum is 1/4000-1/8000 for biotin-streptavidin/HRP technique.
The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Schipper and Tilders, 1983). Results showed that the 5-HIAA antibody dose dependently stained 5-HIAA but did not stain any concentration of 5-HT or 5-HTP. The antiserum was also tested by pre-adsorption at 25 µg/mL with various BSA conjugates. While pre-adsorption with 5-HIAA conjugate completely eliminates immunolabeling, pre-adsorption with conjugates of 5-HT,5-HTP and dopamine had no effect on staining intensity or distribution of stain.
Photo Description: IHC image of neurons staining for 5-H1AA in the raphe nucleus of the rat brainstem. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity/Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: : Rat, Tritonia Diomedea (Sea Slug)
Alternate Names: anti-5-HIAA
Gene Symbol: SLC6A4
Entrez Gene ID: 6532
NCBI Gene Aliases: 5-HTT,5-HTTLPR,5HTT,HTT,OCD1,SERT,hSER
|PROD #||ANTIBODY||CITATION||SPECIES REACTIVITY||APPL METHOD||RESEARCH AREA||# CITED BY||PUBMED ID|
|24274||5-HIAA (5-Hydroxyindoleacetic Acid) Antibody||Appel, NATHAN M., JOSEPH F. Contrera, and ERROL B. De Souza. "Fenfluramine selectively and differentially decreases the density of serotonergic nerve terminals in rat brain: evidence from immunocytochemical studies." Journal of Pharmacology and Experimental Therapeutics 249.3 (1989): 928-943.||Rat||99||2732954|
|24274||5-HIAA (5-Hydroxyindoleacetic Acid) Antibody||J Neurochem. 2005 Dec;95(5):1495-503. Epub 2005 Oct 7. In vivo interaction between serotonin and galanin receptors types 1 and 2 in the dorsal raphe: implication for limbic seizures. Mazarati AM1, Baldwin RA, Shinmei S, Sankar R.||Rat, Mouse||IF||16219029|
|24274||5-HIAA (5-Hydroxyindoleacetic Acid) Antibody||Fickbohm, David J., Nadja Spitzer, and Paul S. Katz. "Pharmacological manipulation of serotonin levels in the nervous system of the opisthobranch mollusc Tritonia diomedea." The Biological Bulletin 209.1 (2005): 67-74.||Tritonia Diomedea (Sea Slug)||IHC||7||16110094|
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