Neuropeptide Y Antibody - 22940

$250.00

Product Overview:

The histochemical antiserum to neuropeptide Y is generated in rabbit against synthetic porcine neuropeptide Y conjugated to methylated bovine serum albumin. The antibody is provided as 100 µL of lyophilized whole serum. It has been used to detect neuropeptide Y in a wide range of species including fish, newt, frog, bird, rat, cat, and human (1-7). Neuropeptide Y (NPY) is a member of a regulatory peptide family and has a marked sequence homology with pancreatic polypeptide (PP) and peptide YY (PYY), which are other members of the family.

In the rat central nervous system, immunohistochemistry has found NPY-like cell bodies in the cortex, caudate-putamen, hypothalamus (arcuate nucleus), hippocampus, anterior olfactory bulb, nucleus accumbens, amygdaloid complex and periaqueductal grey. NPY-like fibers and terminals are detected in high numbers in the bed nucleus of the stria terminalis, the peri- and paraventricular regions of the hypothalamus and thalamus and in discrete hypothalamic nuclei, particularly the suprachiasmatic nucleus.

Controls:

In rat central nervous system, the antiserum has a proven 4+ staining at a 1/2000-1/4000 dilution using the Biotin-Streptavidin/HRP detection method.

All staining is blocked by pre-absorption of the diluted antiserum with excess NPY (10^-6 M). Cross reactivity experiments in which diluted NPY antiserum was absorbed with excess (10^-6M) peptide YY, avian pancreatic polypeptide, ß-endorphin, vasoactive intestinal peptide, cholecystokinin or somatostatin showed no affect in blocking the intensity of staining.

Host: Rabbit	Quantity / Volume: 100 µL
State: lyophilized

Download Product Spec Sheet: Neuropeptide Y Antibody Spec Sheet

Journal References:

Cortical Inhibition Modified by Embryonic Neural Precursors Grafted into the Postnatal Brain , Alvarez-Dolado, Calcagnotto, Karkar, Southwell, Jones-Davis, Estrada, Rubenstein, Alvarez-Buylla, and Baraban , The Journal of Neuroscience, 26(28), 7380-7389, 2006.
Effect of centrally administered C75, a fatty acid synthase inhibitor, on ghrelin secretion and its downstream effects, Hu, Cha, Haasteren, Wang, Lane, Proceedings of the National Academy of Science of the United States of America, 102(11), 3972-3977, 2005.
Effect of centrally administered C75, a fatty acid synthase inhibitor, on ghrelin secretionand its downstream effects, Zhiyuan Hu, Seung Hun Cha, Goedelle van Haasteren, Jing Wang, and M. Daniel Lane*†, PNAS, 102 (11), 3972–3977, 2005.
Gfi1 functions downstream of Math1 to control intestinal secretory cell subtype allocation and differentiation, Shroyer, Wallis,Venken ,Bellen, Zoghbi , GENES & DEVELOPMENT, 19, 2412-2417, 2005.
Immunocytochemical study on the liver innervation in patients with cirrhosis., Stoyanova II, Gulubova MV, acta histochem, 102, 391-402, 2000.
Intrinsic Bias and Lineage Restriction in the Phenotype Determination of Dopamine and Neuropeptide Y Amacrine Cells, Moody SA, Chow I, Huang S, The Journal of Neuroscience, 20(9), 3244-3253, 2000.
Origin and Molecular Specification of Striatal Interneurons, Marin O, Anderson SA, Rubenstein JLR, The Journal of Neuroscience, 20(16), 6063-6076, 2000.
Origins of Cortical Interneuron Subtypes, Xu Qing, Cobos Inma, De La Cruz Estanislao , Rubenstein John L, Anderson Stewart A , The Journal of NeuroScience, 24(11), 2612-2622, 2004.
Sonic hedgehog maintains the identity of cortical interneuron progenitors in the ventral telencephalon, Qing Xu1,*, Carl P. Wonders2,* and Stewart A. Anderson1,†, Development , 132 (22), 4987-4998, 2005.
Spatiotemporal Patterns of Activity in an Intact Mammalian Network with Single-Cell Resolution: Optical Studies of Nicotinic Activity in an Enteric Plexus, Obaid AL, Koyano T, Lindstom J , The Journal of Neuroscience, 19(8), 3073-3093, 1999.