VIP (Vasoactive Intestinal Peptide) Antibody

VIP (Vasoactive Intestinal Peptide) Antibody
Click to enlarge
Price: $275.00
Product ID : 20077
E-mail to a friend



The antibody has a proven strong Biotin-Streptavidin/HRP staining at a 1/4000-1/6000 dilution in rat amygdala, cortex, and suprachiasmatic nucleus.

The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10-5 M. VIP immunolabeling was completely abolished by pre-adsorption with VIP. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: Secretin, gastric inhibitory polypeptide, somatostatin, glucagon, insulin, ACTH, gastrin 34, FMRF-amide, rat GHRF, human GHRF, peptide histidine isoleucine 27, rat pancreatic polypeptide, motilin, peptide YY, substance P, neuropeptide Y, and CGRP.

The histochemical antibody for VIP is generated in a rabbit against porcine VIP conjugated to bovine thyroglobulin with carbodiimide. The antibody is provided as 100 µL of lyophilized whole serum, and 0.09% sodium azide.

Photo Description: IHC image of rat cortex (above) and low magnification image of rat striatum and amygdala staining for VIP (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

VIP Amygdala striatum #20077


Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Reacts With: Avian (Bird), Bat, Bubalus Bubalis (Buffalo), Bullfrog, Canine, Cat, Chick, Chicken, Dog, Dove, Fish, Fowl, Fox, Frog, Gilt (Pig), Goldfish, Guinea Pig, Hamster, Hedgehog, Hen, Human, Lizard, Mink, Mole, Mollusk, Monkey, Mouse, Mudpuppy (Necturus Maculosus), Ovine, Pig, Pigeon, Platypus, Possum, Rabbit, Raccoon, Ram, Rat, Scorpaena Porcus (Fish), Sea Lamprey, Sheep, Shrew, Snake, Sparrow, Sparus Aurata (Fish), Squirrel, Starfish, Sting Ray, Suncus Murinus, Tadpole, Turkey, Turtle, Worm (Spionidae), Zebra Finch (Bird), Zebrafish

Availability: In Stock

Preabsorption Control: Available at Sigma Aldrich, Catalog # V6130

Alternate Names:  Vasoactive intestinal polypeptide; VIP peptides   

Gene Symbol / ID, Accession #:  VIP,7432


Download Product Spec. Sheet: VIP (Vasoactive Intestinal Peptide) Antibody


Journal References:

Postsynaptic targets of somatostatin-containing interneurons in the rat basolateral amygdala; Muller JF, Mascagni F, McDonald AJ.; J Comp Neurol., 500(3):513-29, 2007, Tested in Rat, Cites Diasorin AKA ImmunoStar, PubMed ID: 17120289

Heterogeneous expression of gamma-aminobutyric acid and gamma-aminobutyric acid-associated receptors and transporters in the rat suprachiasmatic nucleus; Belenky MA, Yarom Y, Pickard GE.; J Comp Neurol.,506(4):708-32, 2008, Tested in Rat, IFC, Cites Diasorin AKA ImmunoStar, PubMed ID: 18067149

Immunohistochemical Studies on the Distribution and Origin of Candidate Peptidergic Primary Afferent Neurotransmitters in the Spinal Cord of an Elasmobranch Fish, the Atlantic Stingray; Ritchie TC, Leonard RB.; J. Comp. Neurol,213(4):414-25, 1983, Tested in Sting Ray, Cites ImmunoNuclear AKA ImmunoStar, PubMed ID: 6187783

The autonomous innervation of the buffalo (Bubalus bubalis) testis. An immunohistochemical study; Girolamo P, Costagliola C, Lucini C, Gargiulo G, Castaldo L; Eur J Histochemistry, 47(2):159-164, 2003, Tested in Buffalo, Cites Incstar AKA ImmunoStar, PubMed ID: 12777213

Chicken suprachiasmatic nuclei: II. Autoradiographic and immunohistochemical analysis; Cantwell EL, Cassone VM.; J Comp Neurol., 499(3):442-57, 2006, Tested in Chicken, Cites Incstar AKA ImmunoStar, PubMed ID: 16998905

Localization of a Suprachiasmatic Nucleus Subregion Regulating Locomotor Rhythmicity; LeSauter J, Silver R; The Journal of Neuroscience, 19(13):5574-5585, 1999, Tested in Hamster, Cites Incstar AKA ImmunoStar, PubMed ID: 10377364

Norepinephrine Facilitates the Development of the Murine Sweat Response But Is Not Essential; Tafari AT, Thomas SA, Palmiter RD; The Journal of Neuroscience, 17(11):4275-4281, 1997, Tested in Mouse, Cites Incstar AKA ImmunoStar, PubMed ID: 9151744

Suprachiasmatic nucleus organization.; Moore RY, Speh JC, and Leak RK; Cell Tissue Res, 309:89-98, 2002, Tested in Rat, Cites Incstar AKA ImmunoStar, PubMed ID: 12111539

Spatiotemporal Patterns of Activity in an Intact Mammalian Network with Single-Cell Resolution: Optical Studies of Nicotinic Activity in an Enteric Plexus; Obaid AL, Koyano T, Lindstom  J, Sakai T, Salzberg BM; The Journal of Neuroscience, 19(8):3073-3093, 1999, Tested in Guinea Pig, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 10191324

Synaptic density, convergence, and dendritic complexity of prevertebral sympathetic neurons; Gibbins IL, Teo EH, Jobling P, Morris JL; J Comp Neurol, 455(3):285-298, 2002, Tested in Guinea Pig, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 12483682

Catecholaminergic, cholinergic and peptidergic innervation; ; Cell Tissue Res. 1999 Nov;298(2):275-86., 1999, Tested in Pig, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 10571116

The effects of age on the overall population and on subpopulations of myenteric neurons in the rat small intestine; Johnson RJR, Schemann M, Santer RM, Cowen T; J Anatomy,192(4):479, 1998, Tested in Rat, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 9723975

Noradrenergic Excitation and Inhibition of GABAergic Cell Types in Rat Frontal Cortex; Kawaguchi Y, Shindou T; The Journal of Neuroscience,18(17):6963-6976, 1998, Tested in Rat, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 9712665

Distribution of vanilloid receptors in the rat laryngeal innervation.; Koike S, Uno T, Bamba H, Shibata T, Okano H, Hisa Y; Acta Oto-Laryngologica, 2004, Tested in Rat, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 15224886

The mouse olfactory peduncle; Brunjes PC, Kay RB, Arrivillaga JP.; J Comp Neurol.,518(14):2870-86, 2011, Tested in Mouse, PubMed ID: 21618219

Distinct cis-Regulatory Elements from the Dlx1,Dlx2 Locus; Noe¨l Ghanem, Man Yu, Jason Long, Gary Hatch, John L. R. Rubenstein, and Marc Ekker; The Journal of Neuroscience, 27(19):5012–5022, 2007, Tested in Mouse, PubMed ID: 17494687

Age-Related Decline in VIP-Positive Parasympathetic Nerve Fibers in the Human Submacular Choroid; Monica M. Jablonski,, Alessandro Iannaccone, Drew H. Reynolds, Preston Gallaher, Shaun Allen, XiaoFei Wang, and Anton Reiner; Invest Ophthalmol Vis Sci.,48(2):479-485, 2007, Tested in Human , PubMed ID: 17251439

Direct visual and circadian pathways target neuroendocrine cells in primates; Alfonso Abizaid, Balazs Horvath, David L. Keefe, Csaba Leranth, and Tamas L. Horvath; European Journal of Neuroscience,,20:2767–2776, 2004, Tested in Monkey, PubMed ID: 15548220

Foxa1 and Foxa2 Control the Differentiation of Goblet and Enteroendocrine L- and D-Cells in Mice; Diana Z. Ye, Klaus H. Kaestner; Gastroenterology,8:1-43, 2009, Tested in Mouse, PubMed ID: 19737569

Laminar Specificity of Functional Input to Distinct Types of Inhibitory Cortical Neurons; Xiangmin Xu and Edward M. Callaway; Journal of Neuroscience,,29(1):70–85, 2009, Tested in Mouse, PubMed ID: 19129386

Anatomical Evidence for Enteric Neuroimmune Interactions in Peyer’s Patches; Lucy Vulchanova, Melissa A. Casey, Gwen W. Crabb, William R. Kennedy, and David R. Brown; J Neuroimmunol.,185:64-74, 2007, Tested in Pig, PubMed ID: 17363074

Persistence of sleep-temperature coupling after suprachiasmatic nuclei lesions in rats; Baker, Angara, Szymusiak, and McGinty; Am J Physiol Regul Integr Comp Physiol, 289:R827-R838 , 2005, Tested in Rat, PubMed ID: 15860650

Neuroendocrine correlates of behavioral polymorphism in white-throated sparrows; Donna L. Maney, Kelly L. Erwin, Christopher T. Goode; Hormones and Behavior, 48:196-206, 2005, Tested in Sparrow, PubMed ID: 15878570

Estradiol Modulates Neural Responses to Song in a Seasonal Songbird; Donna L. Maney, Christopher T. Goode, Henry S. Lange, Sara E. Sanford, Benjamin L. Solomon; The Journal Of Comparative Neurology, 511:173-186, 2008, Tested in Sparrow, PubMed ID: 18770869

Mouse cortical inhibitory neuron type that coexpresses somatostatin and calretinin; Xu X, Roby KD, Callaway EM.; J Comp Neurol., 499(1):144-60, 2006, Tested in Mouse, PubMed ID: 16958092

Central projections of melanopsin-expressing retinal ganglion cells in the mouse; Hattar S, Kumar M, Park A, Tong P, Tung J, Yau KW, Berson DM.; J Comp Neurol.,497(3):326-49, 2006, Tested in Mouse, PubMed ID: 16736474

Inducible and Reversible Clock Gene Expression in Brain Using the tTA System for the Study of Circadian Behavior; Hee-Kyung Hong, Jason L. Chong, Weimin Song, Eun Joo Song, Amira A. Jyawook, Andrew C. Schook, Caroline H. Ko, Joseph S. Takahashi; PLOS Genetics,3:324-338, 2007, Tested in Mouse, PubMed ID: 17319750

Molecular and Electrophysiological Characterization of GFP-Expressing CA1 Interneurons in GAD65-GFP Mice; Corette J. Wierenga, Fiona E. Mullner, Ilka Rinke, Tara Keck, Valentin Stein, Tobias Bonhoeffer; PLoS ONE,5:1-11, 2010, Tested in Mouse, PubMed ID: 21209836

Immunochemical characterization of inhibitory mouse cortical neurons: three chemically distinct classes of inhibitory cells; Xu X, Roby KD, Callaway EM; J Comp Neurol., 518(3):389-404, 2010, Tested in Mouse, PubMed ID: 19950390

Development, Maturation, and Necessity of Transcription; Cassandra VanDunk, Lindsay A. Hunter, and Paul A. Gray; J Neurosci. 2011 Apr 27;31(17):6457-67., 2011, Tested in Mouse, PubMed ID: 21525287

Characterization of type I and type II nNOS-expressing interneurons in the barrel cortex of mouse; Quentin Perrenoud, Hélène Geoffroy, Benjamin Gauthier , Armelle Rancillac, Fabienne Alfonsi Nicoletta Kessaris, Jean Rossier,TaniaVitalis, and Thierry Gallopin; Front Neural Circuit,6:36, 2012, Tested in Mouse, PubMed ID: 22754499

Activation of cortical 5-HT3 receptor-expressing interneurons induces NO mediated vasodilatations and NPY mediated vasoconstrictions; Quentin Perrenoud, Jean Rossier, Isabelle Férézou, Hélène Geoffroy, Thierry Gallopin, Tania Vitalis and Armelle Rancillac; Frontiers in Neural Circuits,6:50, 2012, Tested in Mouse, PubMed ID: 22907992

Brain-derived neurotrophic factor regulates expression of vasoactive intestinal polypeptide in retinal amacrine cells.; Cellerino A, Arango-Gonzales B, Pinzon-Duarte G, Kohler K; J Comp Neurol ,467(1):97-104, 2003, Tested in Rat, PubMed ID: 14574682

Physiological and morphological characterization of parvalbumin-containing interneurons of the rat basolateral amygdala; Rainnie DG, Mania I, Mascagni F, McDonald AJ.; J Comp Neurol.,498(1):142-61, 2006, Tested in Rat, PubMed ID: 16856165

BDNF Up-Regulates α7 Nicotinic Acetylcholine Receptor Levels on Subpopulations of Hippocampal Interneurons; Kerri A. Massey*, Wagner M. Zago, and Darwin K. Berg; Mol Cell Neurosci,33(4):381-388, 2006, Tested in Rat, PubMed ID: 17029981

Increasing proportions of tyrosine hydroxylase-immunoreactive interneurons colocalize with choline acetyltransferase or vasoactive intestinal peptide in the developing rat cerebral cortex; Stephen E. Asmus, Benjamin T. Cocanougher, Donald L. Allen, John B. Boone, Elizabeth A. Brooks, Sarah M. Hawkins, Laura A. Hench, Talha Ijaz, Meredith N. Mayfield; Brain Res,1383:108-19, 2011, Tested in Rat, PubMed ID: 21295554

Structure and Autonomic Innervation of the Swim Bladder in the Zebrafish (Danio rerio); Finnely J, Robertson G, McGee C, Smith F, Croll R; The Journal of Comparative Neurology,495:587-606, 2006, Tested in Zebrafish, PubMed ID: 16498679

Development of the Swimbladder and Its Innervation; G.N. Robertson, C.A.S. McGee, T.C. Dumbarton, R.P. Croll, and F.M. Smith; Journal of Morphology,268:967-985, 2007, Tested in Zebrafish, PubMed ID: 17702001

Neurochemical coding of enteric neurons in adult and embryonic zebrafish (Danio rerio); Uyttebroek L, Shepherd IT, Harrisson F, Hubens G, Blust R, Timmermans JP, Van Nassauw L; J Comp Neurol.,518(21):4419-38, 2010, Tested in Zebrafish, PubMed ID: 20853514

Broadly tuned response properties of diverse inhibitory neuron subtypes in mouse visual cortex; Kerlin AM, Andermann ML, Berezovskii VK, Reid RC.; Neuron,67(5):858-71, 2010, Tested in Mouse, PubMed ID: 20826316

Comment on Facebook

Product Reviews

Login or Register to write a review.
Reviewed by ld2596
03/19/2015 - 01:48:03 PM
Works Great!
We have used this antibody to stain for VIP positive neurons (also stains processes) in frozen tissue sections and whole mount preparations of mouse intestine. We get consistent staining at 1:750.

Tissue prep:
4% PFA/PBS for 1.5 hrs, cryoprotected in 30% sucrose until they sink, OCT embedded, and sectioned.
For whole mounts, we open up intestine and fix in 4% PFA/PBS for 1.5 hrs.

IF protocol:
Rehydrate in PBS, block with HINGS, stain in primary @4C O/N.
Wash in PBST, stain in primary with HINGS and DMSO @4C 48 hrs.

Wash with PBS, stain with Alexafluor secondaries (Invitrogen), wash with PBS, and DAPI mount.
Wash in PBST, stain in secondary with HINGS and DMSO @RT 48 hrs.
Optional: proceed with MeOH dehydration and optical clearing.

Meenakshi Rao, MD PhD
Assistant Professor of Pediatrics
Columbia University Medical Center
New York, NY 10032
Reviewed by user
08/22/2012 - 02:45:39 PM
Worked like a charm
After failed several experiments by using other antibodies, we applied VIP antibody (20077) for mouse brain tissue sections for suprachiasmatic nucleus immunohistochemistry.

It turned out that this antibody worked like a charm on my frozen sections. This is definitely an excellent VIP antibody.

Dr. David Ma, Research scientist
Chandler, AZ