VIP (Vasoactive Intestinal Peptide) Antibody

The antibody has a proven strong fluorescent staining at a 1/200-1/400 dilution and a strong Biotin-Streptavidin/HRP staining at a 1/4000-1/6000 dilution in rat amygdala, cortex, and suprachiasmatic nucleus.

The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10-5 M. VIP immunolabeling was completely abolished by pre-adsorption with VIP. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: Secretin, gastric inhibitory polypeptide, somatostatin, glucagon, insulin, ACTH, gastrin 34, FMRF-amide, rat GHRF, human GHRF, peptide histidine isoleucine 27, rat pancreatic polypeptide, motilin, peptide YY, substance P, neuropeptide Y, and CGRP.

The histochemical antibody for VIP is generated in a rabbit against porcine VIP conjugated to bovine thyroglobulin with carbodiimide. The antibody is provided as 100 µL of lyophilized whole serum, and 0.09% sodium azide.

Host:Rabbit

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Reacts With: Buffalo, Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Rat, Pig, Sparrow, Stingray, Zebrafish

Availability: In Stock

Preabsorption Control: Available at Sigma Aldrich, Catalog # V6130

Alternate Names:  Vasoactive intestinal polypeptide; VIP peptides   

Gene Symbol / ID, Accession #:  VIP,7432

Download Product Spec. Sheet: VIP (Vasoactive Intestinal Peptide) Antibody

Journal References:

Postsynaptic targets of somatostatin-containing interneurons in the rat basolateral amygdala; Muller JF, Mascagni F, McDonald AJ.; J Comp Neurol., 500(3):513-29, 2007, Tested in Rat, Cites Diasorin AKA ImmunoStar, PubMed ID: 17120289

Heterogeneous expression of gamma-aminobutyric acid and gamma-aminobutyric acid-associated receptors and transporters in the rat suprachiasmatic nucleus; Belenky MA, Yarom Y, Pickard GE.; J Comp Neurol.,506(4):708-32, 2008, Tested in Rat, IFC, Cites Diasorin AKA ImmunoStar, PubMed ID: 18067149

Immunohistochemical Studies on the Distribution and Origin of Candidate Peptidergic Primary Afferent Neurotransmitters in the Spinal Cord of an Elasmobranch Fish, the Atlantic Stingray; Ritchie TC, Leonard RB.; J. Comp. Neurol,213(4):414-25, 1983, Tested in Sting Ray, Cites ImmunoNuclear AKA ImmunoStar, PubMed ID: 6187783

The autonomous innervation of the buffalo (Bubalus bubalis) testis. An immunohistochemical study; Girolamo P, Costagliola C, Lucini C, Gargiulo G, Castaldo L; Eur J Histochemistry, 47(2):159-164, 2003, Tested in Buffalo, Cites Incstar AKA ImmunoStar, PubMed ID: 12777213

Chicken suprachiasmatic nuclei: II. Autoradiographic and immunohistochemical analysis; Cantwell EL, Cassone VM.; J Comp Neurol., 499(3):442-57, 2006, Tested in Chicken, Cites Incstar AKA ImmunoStar, PubMed ID: 16998905

Localization of a Suprachiasmatic Nucleus Subregion Regulating Locomotor Rhythmicity; LeSauter J, Silver R; The Journal of Neuroscience, 19(13):5574-5585, 1999, Tested in Hamster, Cites Incstar AKA ImmunoStar, PubMed ID: 10377364

Norepinephrine Facilitates the Development of the Murine Sweat Response But Is Not Essential; Tafari AT, Thomas SA, Palmiter RD; The Journal of Neuroscience, 17(11):4275-4281, 1997, Tested in Mouse, Cites Incstar AKA ImmunoStar, PubMed ID: 9151744

Suprachiasmatic nucleus organization.; Moore RY, Speh JC, and Leak RK; Cell Tissue Res, 309:89-98, 2002, Tested in Rat, Cites Incstar AKA ImmunoStar, PubMed ID: 12111539

Spatiotemporal Patterns of Activity in an Intact Mammalian Network with Single-Cell Resolution: Optical Studies of Nicotinic Activity in an Enteric Plexus; Obaid AL, Koyano T, Lindstom  J, Sakai T, Salzberg BM; The Journal of Neuroscience, 19(8):3073-3093, 1999, Tested in Guinea Pig, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 10191324

Synaptic density, convergence, and dendritic complexity of prevertebral sympathetic neurons; Gibbins IL, Teo EH, Jobling P, Morris JL; J Comp Neurol, 455(3):285-298, 2002, Tested in Guinea Pig, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 12483682

Catecholaminergic, cholinergic and peptidergic innervation; ; Cell Tissue Res. 1999 Nov;298(2):275-86., 1999, Tested in Pig, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 10571116

The effects of age on the overall population and on subpopulations of myenteric neurons in the rat small intestine; Johnson RJR, Schemann M, Santer RM, Cowen T; J Anatomy,192(4):479, 1998, Tested in Rat, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 9723975

Noradrenergic Excitation and Inhibition of GABAergic Cell Types in Rat Frontal Cortex; Kawaguchi Y, Shindou T; The Journal of Neuroscience,18(17):6963-6976, 1998, Tested in Rat, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 9712665

Distribution of vanilloid receptors in the rat laryngeal innervation.; Koike S, Uno T, Bamba H, Shibata T, Okano H, Hisa Y; Acta Oto-Laryngologica, 2004, Tested in Rat, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 15224886

The mouse olfactory peduncle; Brunjes PC, Kay RB, Arrivillaga JP.; J Comp Neurol.,518(14):2870-86, 2011, Tested in Mouse, PubMed ID: 21618219

Distinct cis-Regulatory Elements from the Dlx1,Dlx2 Locus; Noe¨l Ghanem, Man Yu, Jason Long, Gary Hatch, John L. R. Rubenstein, and Marc Ekker; The Journal of Neuroscience, 27(19):5012–5022, 2007, Tested in Mouse, PubMed ID: 17494687

Age-Related Decline in VIP-Positive Parasympathetic Nerve Fibers in the Human Submacular Choroid; Monica M. Jablonski,, Alessandro Iannaccone, Drew H. Reynolds, Preston Gallaher, Shaun Allen, XiaoFei Wang, and Anton Reiner; Invest Ophthalmol Vis Sci.,48(2):479-485, 2007, Tested in Human , PubMed ID: 17251439

Direct visual and circadian pathways target neuroendocrine cells in primates; Alfonso Abizaid, Balazs Horvath, David L. Keefe, Csaba Leranth, and Tamas L. Horvath; European Journal of Neuroscience,,20:2767–2776, 2004, Tested in Monkey, PubMed ID: 15548220

Foxa1 and Foxa2 Control the Differentiation of Goblet and Enteroendocrine L- and D-Cells in Mice; Diana Z. Ye, Klaus H. Kaestner; Gastroenterology,8:1-43, 2009, Tested in Mouse, PubMed ID: 19737569

Laminar Specificity of Functional Input to Distinct Types of Inhibitory Cortical Neurons; Xiangmin Xu and Edward M. Callaway; Journal of Neuroscience,,29(1):70–85, 2009, Tested in Mouse, PubMed ID: 19129386

Anatomical Evidence for Enteric Neuroimmune Interactions in Peyer’s Patches; Lucy Vulchanova, Melissa A. Casey, Gwen W. Crabb, William R. Kennedy, and David R. Brown; J Neuroimmunol.,185:64-74, 2007, Tested in Pig, PubMed ID: 17363074

Persistence of sleep-temperature coupling after suprachiasmatic nuclei lesions in rats; Baker, Angara, Szymusiak, and McGinty; Am J Physiol Regul Integr Comp Physiol, 289:R827-R838 , 2005, Tested in Rat, PubMed ID: 15860650

Neuroendocrine correlates of behavioral polymorphism in white-throated sparrows; Donna L. Maney, Kelly L. Erwin, Christopher T. Goode; Hormones and Behavior, 48:196-206, 2005, Tested in Sparrow, PubMed ID: 15878570

Estradiol Modulates Neural Responses to Song in a Seasonal Songbird; Donna L. Maney, Christopher T. Goode, Henry S. Lange, Sara E. Sanford, Benjamin L. Solomon; The Journal Of Comparative Neurology, 511:173-186, 2008, Tested in Sparrow, PubMed ID: 18770869

Mouse cortical inhibitory neuron type that coexpresses somatostatin and calretinin; Xu X, Roby KD, Callaway EM.; J Comp Neurol., 499(1):144-60, 2006, Tested in Mouse, PubMed ID: 16958092

Central projections of melanopsin-expressing retinal ganglion cells in the mouse; Hattar S, Kumar M, Park A, Tong P, Tung J, Yau KW, Berson DM.; J Comp Neurol.,497(3):326-49, 2006, Tested in Mouse, PubMed ID: 16736474

Inducible and Reversible Clock Gene Expression in Brain Using the tTA System for the Study of Circadian Behavior; Hee-Kyung Hong, Jason L. Chong, Weimin Song, Eun Joo Song, Amira A. Jyawook, Andrew C. Schook, Caroline H. Ko, Joseph S. Takahashi; PLOS Genetics,3:324-338, 2007, Tested in Mouse, PubMed ID: 17319750

Molecular and Electrophysiological Characterization of GFP-Expressing CA1 Interneurons in GAD65-GFP Mice; Corette J. Wierenga, Fiona E. Mullner, Ilka Rinke, Tara Keck, Valentin Stein, Tobias Bonhoeffer; PLoS ONE,5:1-11, 2010, Tested in Mouse, PubMed ID: 21209836

Immunochemical characterization of inhibitory mouse cortical neurons: three chemically distinct classes of inhibitory cells; Xu X, Roby KD, Callaway EM; J Comp Neurol., 518(3):389-404, 2010, Tested in Mouse, PubMed ID: 19950390

Development, Maturation, and Necessity of Transcription; Cassandra VanDunk, Lindsay A. Hunter, and Paul A. Gray; J Neurosci. 2011 Apr 27;31(17):6457-67., 2011, Tested in Mouse, PubMed ID: 21525287

Characterization of type I and type II nNOS-expressing interneurons in the barrel cortex of mouse; Quentin Perrenoud, Hélène Geoffroy, Benjamin Gauthier , Armelle Rancillac, Fabienne Alfonsi Nicoletta Kessaris, Jean Rossier,TaniaVitalis, and Thierry Gallopin; Front Neural Circuit,6:36, 2012, Tested in Mouse, PubMed ID: 22754499

Activation of cortical 5-HT3 receptor-expressing interneurons induces NO mediated vasodilatations and NPY mediated vasoconstrictions; Quentin Perrenoud, Jean Rossier, Isabelle Férézou, Hélène Geoffroy, Thierry Gallopin, Tania Vitalis and Armelle Rancillac; Frontiers in Neural Circuits,6:50, 2012, Tested in Mouse, PubMed ID: 22907992

Brain-derived neurotrophic factor regulates expression of vasoactive intestinal polypeptide in retinal amacrine cells.; Cellerino A, Arango-Gonzales B, Pinzon-Duarte G, Kohler K; J Comp Neurol ,467(1):97-104, 2003, Tested in Rat, PubMed ID: 14574682

Physiological and morphological characterization of parvalbumin-containing interneurons of the rat basolateral amygdala; Rainnie DG, Mania I, Mascagni F, McDonald AJ.; J Comp Neurol.,498(1):142-61, 2006, Tested in Rat, PubMed ID: 16856165

BDNF Up-Regulates α7 Nicotinic Acetylcholine Receptor Levels on Subpopulations of Hippocampal Interneurons; Kerri A. Massey*, Wagner M. Zago, and Darwin K. Berg; Mol Cell Neurosci,33(4):381-388, 2006, Tested in Rat, PubMed ID: 17029981

Increasing proportions of tyrosine hydroxylase-immunoreactive interneurons colocalize with choline acetyltransferase or vasoactive intestinal peptide in the developing rat cerebral cortex; Stephen E. Asmus, Benjamin T. Cocanougher, Donald L. Allen, John B. Boone, Elizabeth A. Brooks, Sarah M. Hawkins, Laura A. Hench, Talha Ijaz, Meredith N. Mayfield; Brain Res,1383:108-19, 2011, Tested in Rat, PubMed ID: 21295554

Structure and Autonomic Innervation of the Swim Bladder in the Zebrafish (Danio rerio); Finnely J, Robertson G, McGee C, Smith F, Croll R; The Journal of Comparative Neurology,495:587-606, 2006, Tested in Zebrafish, PubMed ID: 16498679

Development of the Swimbladder and Its Innervation; G.N. Robertson, C.A.S. McGee, T.C. Dumbarton, R.P. Croll, and F.M. Smith; Journal of Morphology,268:967-985, 2007, Tested in Zebrafish, PubMed ID: 17702001

Neurochemical coding of enteric neurons in adult and embryonic zebrafish (Danio rerio); Uyttebroek L, Shepherd IT, Harrisson F, Hubens G, Blust R, Timmermans JP, Van Nassauw L; J Comp Neurol.,518(21):4419-38, 2010, Tested in Zebrafish, PubMed ID: 20853514

Broadly tuned response properties of diverse inhibitory neuron subtypes in mouse visual cortex; Kerlin AM, Andermann ML, Berezovskii VK, Reid RC.; Neuron,67(5):858-71, 2010, Tested in Mouse, PubMed ID: 20826316