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Vesicular Monoamine Transporter 2 Rabbit Antibody

Vesicular Monoamine Transporter 2 Rabbit Antibody
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Price: $350.00
Product ID : 20042
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Description

The ImmunoStar VMAT2 antiserum was quality control tested using standard immunohistochemical methods in rat brain and adrenal medulla using biotin/avidin-HRP techniques.

Specificity of the antiserum was demonstrated by soluble pre-adsorption and Western blot. Tissue staining is completely eliminated by pretreatment of the diluted antibody with an excess of rat VMAT2 peptide residues 496-515. 

Western blot analysis of immunoprecipitated rat brain homogenates demonstrates a dense immunoreactive band of approximately 55 kD and a minor band of approximately 75 kD.


Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Species Reactivity: Rat

Availability: In Stock

Alternate Names:  Monoamine transporter; Solute carrier family 18 member 2; Synaptic vesicular amine transporter; Vesicular amine transporter 2; Solute carrier family 18 A2 (vesicular monoamine transporter 2); SLC18A2; SVMT; VAT2; Svat; mnat   

Gene Symbol / ID, Accession #:  Slc18a2, 25549


Download Product Spec. Sheet:  Vesicular Monoamine Transporter 2 Rabbit Antibody


Journal References:


None

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Product Reviews

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Reviewed by user
11/15/2013 - 12:08:35 PM
Works Very Well
We have used the VMAT2 antibody from your company a couple of times.

From the results it seems that this antibody works very well: it labels the structures in the brain and the spinal cord where this transporter should be located and labeling is very clear.

Mengliang Zhang, DM, PhD, Associate Professor
University of Copenhagen
Department of Neuroscience and Pharmacology
The Panum Institute, bldg 33.3
Blegdamsvej 3
DK-2200 Copenhagen
Denmark
Reviewed by fulvashah
09/03/2013 - 12:19:18 PM
Excellent Antibody!!!!

I have used this antibody (#20042) to label the brains of adult Mouse. Works very well. I highly recommend. I used the antibody on mouse Striatal and substantia Nigra tissue for fluorescence microscopy. I tried different concentration and 1:2000 works the best. The staining was also done in transgenic mice that express GFP on TH promoter for a positive control. The entire process was done on free-floating sections by keeping sections on the shaker agitating and slow speed.

Day1
Mice were cardiac perfused with fresh 4% Paraformaldehyde and 15% picric acid in 0.1M PB and post fix in same fixative overnight at 4ºC.

Day 2
-Washed brain 3 x 10min in phosphate buffered saline (PBS; pH 7.4)
-Brains were cut @ 50um on vibratome
-Clean sections with 1% sodium borohydride in PBS for 15 min @ RT, followed by 3 x 10min PBS wash.
-Quenched endogenous reaction with 10% Methanol and 3% Hydrogen peroxide in PBS for 15 min @ RT, followed by 3 x 10min PBS wash.
-Blocked with 3% bovine serum albumin (BSA), 10% Normal Donkey serum (NDS) and 0.5% Triton X in PBS 4-6 hours @ RT.

Day 3
- Incubate in primary (Rb anti-VMAT2, 1:2000; Immunostar #20042) in 1% BSA, 1% NDS and 0.1% Triton-X in PBS for 60-70 hours @ 4ºC (This is over the weekend. For fast result, can also incubate for overnight @ RT).

Day 4 or 6
-Wash 3 x 10 min in PBS
- Incubate in secondary (Donkey Anti Rabbit IgG with Alexa 594, 1:400) for 4-6 hours @ RT
-Wash 3 x 10min in PBS
-Mount section and cover slip

Fulva Shah
Laboratory Researcher III
Rutgers University

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