Vasopressin Antibody

Vasopressin Antibody
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Price: $250.00
Product ID : 20069
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The antibody has a proven strong biotin-streptavidin/HRP staining at a 1/2000 - 1/4000 dilution in rat hypothalamus.

Staining is completely eliminated by pretreatment of the diluted antibody with 10 µg/mL of arginine vasopressin. Pre-adsorption with as much as 100 µg/mL of oxytocin had no effect on immunolabeling.

Photo Description: IHC image of rat hypothalamus staining for vasopressin. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. 

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Chicken, Hamster, Mouse, Rat

Availability: In Stock

Alternate Names:  Vasopressin (Arg); Antidiuretic Hormone; Arginine vasopressin; Vasopressin-neurophysin 2-copeptin; ARG-vasopressin; prepro-AVP-NPII; neurohypophyseal; vasopressin-neurophysin II-copeptin; prepro-arginine-vasopressin-neurophysin II; AVP-NPII; VP; ADH; ARVP; AVRP   

Gene Symbol / ID, Accession #:  AVP, 551


Download Product Spec. SheetVasopressin Antibody


Journal References:

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Kawata, M., et al. "Differences in the distributional pattern of CRF-, oxytocin-, and vasopressin-immunoreactive nerve fibers in the median eminence of the rat." Cell and tissue research 230.2 (1983): 247-258. Tested in Rat, PubMed ID: 6342799

Pelletier, G. E. O. R. G. E. S. "Identification of ending containing dopamine and vasopressin in the rat posterior pituitary by a combination of radioautography and immunocytochemistry at the ultrastructural level." Journal of Histochemistry & Cytochemistry 31.4 (1983): 562-564. Tested in Rat, PubMed ID: 6827086

BASKIN, DENIS G., et al. "Immunocytochemical Detection of Insulin in Rat Hypothalamus and Its Possible Uptake from Cerebrospinal Fluid." Endocrinology 113.5 (1983): 1818-1825. Tested in Rat, PubMed ID: 6354698

Leccese, Arthur P., and John L. Isenhour. "Intraventricular administration of antivasopressin serum inhibits retention in mice." Peptides 4.2 (1983): 265-267. Tested in Mouse, PubMed ID: 6622289

In the News:

Vasopressin Antibody on the cover of Science

Dark-field photomicrograph of the anterior hypothalamus of a hamster immunostained with antibodies to vasopressin, showing the paired suprachiasmatic nuclei (SCN) between the third ventricle and optic chiasm (top) and the magnocellular vasopressin cells (yellow fireworks) of the paraventricular nucleus, above which is the SCN. The SCN, the locus of the mammalian biological clock, can be identified as two oval structures at the base of the brain (toward title). See page 975. [Photographic image by Russell Foster]

ImmunoStar's Vasopressin Antibody

Transplanted Suprachiasmatic Nucleus Determines Circadian Period. MARTIN R. RALPH, RUSSELL G. FOSTER, FRED C. DAVIS, MICHAEL MENAKER.  23 February 1990 vol 247, issue 4945, pages 899-989.

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Product Reviews

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Reviewed by user
12/18/2012 - 01:11:07 PM
Beautiful cells
We have used your Vasopressin antibody (Product ID: 20069) in many of our studies and have found in to be a very specific and clear antibody. The staining is great and the cells are beautiful.

We use a total 1:5000 concentration. It is mixed with 10% serum, 10% biotin diluted in 80% PBS-T and incubated for 48-72 hours at 4 degrees C. We used brain tissue from Thamnophis sirtalis (Red-sided garter snake).

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Ashley Maine
Lutterschmidt Lab
Portland State University
Department of Biology
Portland, OR 97207