Substance P Antibody

Substance P Antibody
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Price: $275.00
Product ID : 20064
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The ImmunoStar Substance P antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat substantia nigra and spinal cord using biotin/avidin-HRP techniques. Recommended primary dilution is 1/6000-1/8000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique.

The specificity of the antiserum for Substance P was demonstrated using soluble pre-adsorption with the peptides in question at a final concentration of 10 µg of peptide per mL of diluted antiserum. Substance P immunolabeling was completely abolished by pre-adsorption with Substance P. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: neurokinin A, neurokinin B, somatostatin and neuropeptide K.

Photo Description: IHC image of rat spinal cord (above) and rat tegmentum (below) staining for substance p. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning for brain and frozen sectioning for spinal cord. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Substand P tegmentum #20064

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Bird, Buffalo, Chicken, Dog, Equine, Frog, Gerbil, Guinea Pig, Human, Monkey, Mouse, Pig, Rat, Sheep, Stingray, Zebra Finch

Availability: In Stock

Alternate Names:  Protachykinin-1; PPT; tachykinin peptide hormone; NKA, NKNA, TAC2; tachykinin, precursor 1   

Gene Symbol / ID, Accession #:  TAC1, 6863


Download Product Spec. Sheet Substance P Antibody


Journal References:

Immunohistochemical Studies on the Distribution and Origin of Candidate Peptidergic Primary Afferent Neurotransmitters in the Spinal Cord of an Elasmobranch Fish, the Atlantic Stingray, Ritchie TC, Leonard RB., J. Comp. Neurol, 213(4), 414-25, 1983, sting ray, PubMed: 6187783

Neonatal capsaicin treatment attenuates spinal Fos activation and dynorphin gene expression following peripheral tissue inflammation and hyperalgesia., Hylden JL, Noguchi K, Ruda MA., Journal Neuroscience, 12(5), 1716-1725, 1992, rat, PubMed: 1374461

A sex difference in the pattern of substance P-like immunoreactivity in the bed nucleus of the stria terminalis, Malsbury CW, McKay K., Brain Research, 420(2), 365-370, 1987, rat, PubMed: 2445435

Developmentally regulated and evolutionarily conserved expression of SLITRK1 in brain circuits implicated in Tourette syndrome, , Journal of comparative neurology, 513(1), 21-37, 2009, Human, rat, mouse, PubMed: 19105198

Normal development and effects of early deafferentation on choline acetyltransferase, substance P and serotonin-like immunoreactivity in the interpeduncular nucleus., Barr GA, Eckenrode TC, Murray M., Brain Research, 418(2), 301-313, 1987, rat, PubMed: 2445414

Sex Difference in the Substance P-Immunoreactive Innervation of the Medial Nucleus of the Amygdala, Malsbury CW, McKay K., Brain Research Bulletin, 23(6), 561-567, 1989, rat, PubMed: 2482114

20064, SUBSTANCE P, Chicken suprachiasmatic nuclei: II. Autoradiographic and immunohistochemical analysis, Cantwell EL, Cassone VM., J Comp Neurol., 499(3), 442-57, 2006, Chicken, PubMed: 16998905

Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system., Reynolds BA, Weiss S., Science, 255(5052), 1707-1710, 1992, mouse, PubMed: 1553558

Modification of classical neurochemical markers in identified primary afferent neurons with Abeta-, Adelta-, and C-fibers after chronic constriction injury in mice, Ruscheweyh R, Forsthuber L, Schoffnegger D, Sandkühler J., J Comp Neurol., 502(2), 325-36, 2007, Mouse, PubMed: 17348016

Expression of P2X3 receptor in the trigeminal sensory nuclei of the rat, Kim YS, Paik SK, Cho YS, Shin HS, Bae JY, Moritani M, Yoshida A, Ahn DK, Valtschanoff J, Hwang SJ, Moon C, Bae YC., J Comp Neurol., 506(4), 627-39, 2008, Rat, PubMed: 18067147

Sympathetic innervation of the ileocecal junction in horses, Russo D, Bombardi C, Grandis A, Furness JB, Spadari A, Bernardini C, Chiocchetti R., J Comp Neurol., 518(19), 4046-66, 2010, Equine, PubMed: 20737599

Investigating the role of substance P in photic responses of the circadian system: Individual and combined actions with gastrin-releasing peptide, Roxanne Sterniczuk a, Mark A. Colijn a, Maria Nunez &a, Michael C. Antle a,b, Neuropharmacology, 1–9, 2009, hamster

Charactizaton of sheep (ovis aries) palatine tonsil inervation, D. Russo, Mongardi Fantaguzzi,  Di Guardo, Clavenzani, G. Lalatta Costerbosa. Ligios And R.  Chiocchetti, Neuroscience, 161, 813–826, 2009, sheep

Substance P and acetylcholine are co-localized in the pathway mediating mucociliary activity in Rana pipiens, C. J. Hernández, T. Ortíz, C. Rosa K. Foster, M Tyagi, N. Lugo, R. Albrecht, and S. Chinapen, Comp Biochem Physiol B Biochem Mol Biol, 146(4), 477–481, 2007, loepard frog

Neurochemical diversity of afferent neurons that transduce sensory signals from dog ventricular myocardium, Donald B. Hoover, Angela V. Shepherd, E. Marie Southerland, J. Andrew Armour, and Jeffrey L. Ardell, Auton Neurosci., 141(1-2), 38–45, 2008, mongrel dog

Reticulospinal Neurons In The Pontomedullary Reticular Formation Of The Monkey (Macaca fascicularis), S. T. Sakai, A. G. Davidson, And J. A. Buford, Neuroscience, 7.036, 1-13, 2009, monkey

A Role for L-Type Calcium Channels in Developmental Regulation of Transmitter Phenotype in Primary Sensory Neurons, Brosenitsch TA, Salgado-Commissariat D, Kunze DL, The Journal of Neuroscience, 18(3), 1047-1055, 1998.

Altered parvalbumin-positive neuron distribution in basal ganglia of individuals with Tourette Syndrome, Kalanithi, Zheng, Kataoka, DiFiglia, Grantz, Saper, Schwartz, Leckman, and Vaccarino, Proceedings of the National Academy of Science of the United States of America, 102(37), 13307-13312, 2005.
An immunohistochemical and pathway tracing study of the striatopallidal organization of area x in the male zebra finch., Reiner A, Laverghetta AV, Meade CA, et al, J Comp Neurol , 469, 239-261, 2004.
Axotomy Upregulates the Anterogreade Transport and Expression of Brain-Derived Neurotrophic Factor by Sensory Neurons, Tonra JR, Curtis R, Wong V, The Journal of Neuroscience, 18(11), 4374-4383, 1998.Depletion of substance P and glutamate by capsaicin blocks respiratory rhythm in neonatal rat in vitro., Morgado-Valle C, Feldman JL, J of Physiology, 555(3), 783-16, 2004.

Distribution of cocaine- and amphetamine-regulated transcript peptide in the guinea pig intrinsic cardiac nervous system and colocalization with neuropeptides or transmitter synthetic enzymes., Calupca MA, Locknar SA, Zhang L, Harrison TA, et al, J Comp Neurol , 439(1), 73-86, 2001.
Identification of the Anterior Nucleus of the Ansa Lenticularis in Birds as the Homolog of the Mammalian Subthalamic Nucleus, Jiao Y, Medina L, Veenman LC , The Journal of Neuroscience, 20(18), 6998-7010, 2000.Immunocytochemical study on the liver innervation in patients with cirrhosis., Stoyanova II, Gulubova MV, acta histochem, 102, 391-402, 2000.
Immunohistochemical Analysis of Primary Sensory Neurons Latently Infected with Herpes Simplex Virus Type 1, Yang L, Voytek CC , Margolis TP, Journal of Virology, 74(1), 209-217, 2000.
Intradermal Injection of Capsaicin in Humans Produces Degeneration and Subsequent Reinnervation of Epidermal Nerve Fibers: Correlation with Sensory Function, Simone DA, Nolano M, Wendelschafer-Crabb G, The Journal of Neuroscience, 18(21), 8947-8959, 1998.
Loss of interstitial cells of cajal and inhibitory innervation in insulin-dependent diabetes., He C, Soffer EE, Ferris CD, Walsh RM, et al, Gastroenterolgy, 121, 427-434, 2001.
Multipotent CNS Stem Cells Are Present in the Adult Mammalian Spinal Cord and Ventricular Neuroaxis, Weiss S, Dunne C , Hewson J, The Journal of Neuroscience, 16(23), 7599-7609, 1996.
Specificity in the Projections of Prefrontal and Insular Cortex to Ventral Striatopallidum and the Extended Amygdala , Reynolds and Zahm , The Journal of Neuroscience, 25(50), 11757-11767, 2005.
The autonomous innervation of the buffalo (Bubalus bubalis) testis. An immunohistochemical study., Girolamo P, Costagliola C, Lucini C, Gargiulo G, Castaldo L, Eur J Histochemistry, 47(2), 159-164, 2003.
The Generation of Neuronal Heterogeneity in a Rat Sensory Ganglion, Hall AK, Ai X, Hickman GE, The Journal of Neuroscience, 17(8), 2775-2784, 1997.
Vanilloid receptor VR1-positive afferents are distributed differently at different levels of the rat lumbar spinal cord, Hwang, Valtschanoff, Neuroscience Letters, 349, 41-44, 2003.

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Product Reviews

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Reviewed by droot
07/10/2012 - 10:09:39 PM
Ventral pallidum visualization
For our manuscript, Differential roles of ventral pallidum subregions during cocaine self-administration behaviors, in press at Journal of Comparative Neurology, we used the Immunostar rabbit anti-substance P primary antibody to visualize the entire ventral pallidum.

Rats were perfused with saline followed by 4% PF, stored in 30% sucrose, and coronally sectioned to 40 um. We followed the protocols of Zahm/Heimer and colleagues when they discovered the ventral pallidum subregions and their afferent/efferent projection patterns.

The protocol consisted of the following steps:
1. Wash in 0.1M phosphate buffer (PB; pH 7.4)
2. 15 min in 1% sodium borohydride
3. Wash
4. 1 hour blocking with PB containing 0.1% Triton X-100 and 3% normal goat serum
5. Primary antibody overnight at 4C - ImmunoStar rabbit anti-substance P diluted 1 : 6500 in PB containing 0.1% Triton X-100 and 3% normal goal serum
6. Wash
7. 1 hour anti-rabbit biotinylated secondary (Vector) 1 : 200 in PB with 0.1% Triton X-100
8. Wash
9. 1 hour ABC (Vector) 1 : 200 in PB with 0.1% Triton X-100
10. Wash
11. Develop with 0.05% DAB for 6 min

Our study involved recording neurons within the ventral pallidum subregions during specific aspects of intravenous cocaine self-administration (approaching toward, responding on, or retreating away from a cocaine-reinforced operandum). In order to verify the placement of microwires within the VP subregions, prior to perfusion we passed current through each stainless steel microwire to leave an iron deposit at the uninsulated tip. After DAB (brown reaction) and mounting, we visualized the iron deposit by incubating in a 5% potassium ferrocyanide and 10% HCl solution (leaving a blue-green reaction).

For our purposes, this antibody strongly labeled fibers in VP with low background similar to what Haber and Nauta 1983 reported.

David H. Root, Ph.D.
Rutgers University
Reviewed by Dan_Tylee
04/05/2012 - 01:07:22 PM
Strong Staining of Fibers
Tissue: Maccaca fasciscularis, perfused with saline and 4% paraformaldehyde, dehydrated through increase sucrose gradients, sectioned at 40 um.

Method: Immunocytochemistry on free floating sections. Incubated with ImmunoStar's primary antibody at a concentration of 1:5000 for 4 days in 10% normal goat serum (in 0.1M PO4 buffer with .3% Triton X-100). Rinsed, then incubated with Vector Lab's biotinylated goat anti-rabbit secondary antibody (#BA-1000) at a concentration of 1:200 for 40 minutes at room temperature in the same 10% normal goat serum solution. Rinsed, then incubated with Vector's standard peroxidase kit (#PK-4000) for 60 minutes at room temperature in 0.1M PO4 buffer with 0.3% Triton X-100. Rinsed, then developed using the instructions for the kit.

Results: Low background. Strong staining of fibers, especially in the pallidum. Clear fiber morphology.

Daniel Tylee
Department of Neurobiology & Anatomy
University of Rochester - School of Medicine and Dentistry