Somatostatin Antibody

Somatostatin Antibody
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Price: $250.00
Product ID : 20067
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The antibody has a proven strong indirect immunofluorescent staining at a 1/400-1/800 dilution and strong Biotin-Streptavidin/HRP staining at a 1/1000-1/2000 dilution in rat hypothalamus (median eminence). The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 106M.

Somatostatin immunolabeling was completely abolished by pre-adsorption with somatostatin, somatostatin 25, and somatostatin 28. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: substance P, amylin, glucagon, insulin, neuropeptide Y, and VIP.

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Amphibian, Chicken, Fish, Frog, Human, Monkey, Mouse, Rat, Stingray

Availability: In Stock

Preabsorption Control: Available at Sigma Aldrich, Catalog # S9129

Alternate Names:  Growth hormone release-inhibiting factor; Somatostatin 14; Somatostatin 28; prepro-somatostatin; SMST; somatostatin   

Gene Symbol / ID, Accession #:  SST, 6750

Download Product Spec. SheetSomatostatin Antibody

Journal References:

Delta opioid receptors colocalize with corticotropin releasing factor in hippocampal interneurons; Tanya J. Williams and Teresa A. Milnera,; Neuroscience, 179:9-22, 2011, Tested in Rat, IFC, Cites Diasorin AKA ImmunoStar, PubMed ID: 21277946

Distributions of certain neuropeptides in the primate thalamus; Molinari M, Hendry SH, Jones EG.; Brain Research, 426(2):270-289, 1987, Tested in Monkey, Cites ImmunoNuclear AKA ImmunoStar, PubMed ID: 2446708

Immunohistochemical Studies on the Distribution and Origin of Candidate Peptidergic Primary Afferent Neurotransmitters in the Spinal Cord of an Elasmobranch Fish, the Atlantic Stingray; Ritchie TC, Leonard RB.; J. Comp. Neurol, 213(4):414-25, 1983, Tested in Sting Ray, Cites ImmunoNuclear AKA ImmunoStar, PubMed ID: 6187783

Chicken suprachiasmatic nuclei: II. Autoradiographic and immunohistochemical analysis; Cantwell EL, Cassone VM.; J Comp Neurol., 499(3):442-57, 2006, Tested in Chicken, Cites Incstar AKA ImmunoStar, PubMed ID: 16998905

Ontogenetic distribution of the transcription factor Nkx2.2 in the developing forebrain of Xenopus laevis; Laura Domínguez, Agustín González and Nerea Moreno; Front Neuroanat, 5:1-13, 2010, Tested in Frog, Cites Incstar AKA ImmunoStar, PubMed ID: 21415915

Immunocytochemical study on the liver innervation in patients with cirrhosis.; Stoyanova II, Gulubova MV; acta histochem, 102:391-402, 2000, Tested in Human , Cites Incstar AKA ImmunoStar, PubMed ID: 11145532

Tissue-specific and Developmental Regulation of the Rat Insulin II Gene Enhancer, RIPE3, in Transgenic Mice; Stellrech CMM, DeMayo FJ, Finegold ; The Am Soc for Biochemistry and Mol Biology, 272(6):3567-3572, 1997, Tested in Mouse, Cites Incstar AKA ImmunoStar, PubMed ID: 9013607

Dexamethasone Induces Neuropeptide Y (NPY) Expression and Impairs Insulin Release in the Insulin-producing Cell Line RINm5F; Myrsen-Axcrona U, Karlsson S, Sundler F; The Am Soc for Biochemistry and Mol Biology, 272(16):10790-10796, 1997, Tested in Rat, Cites Incstar AKA ImmunoStar, PubMed ID: 9099732

Suprachiasmatic nucleus organization.; Moore RY, Speh JC, and Leak RK+D414; Cell Tissue Res, 309:89-98, 2002, Tested in Rat, Cites Incstar AKA ImmunoStar, PubMed ID: 12111539

Hodological characterization of the medial amygdala in anuran amphibians.; Moreno N, Gonzalez A; J Comp Neurol , 466(3):389-408, 2003, Tested in Amphibian, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 14556296

Spatiotemporal patterns of secretomotor neuron generation in the parvicellular neuroendocrine system; Markakis EA, Swanson LW; Brain Res Brain Res Rev., 24(2-3):255-91, 1997, Tested in Rat, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 9385456

The effects of age on the overall population and on subpopulations of myenteric neurons in the rat small intestine; Johnson RJR, Schemann M, Santer RM, Cowen T; J Anatomy, 192(4):479, 1998, Tested in Rat, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 9723975

The mouse olfactory peduncle; Brunjes PC, Kay RB, Arrivillaga JP.; J Comp Neurol., 518(14):2870-86, 2011, Tested in Mouse, PubMed ID: 21618219
20067    The Organization of the Inner Retina in a Pure-Rod Deep-Sea Fish; Hirt, Wagner; Brain, Behavior, and Evolution,65:157-167, 2005, Tested in Fish, PubMed ID: 15677861

Cre Recombinase-mediated Gene Deletion in Layer 4 of Murine Sensory Cortical Areas; Guey-Ying Liao and Baoji Xu; Genesis, 46(6):289–293, 2008, Tested in Mouse, PubMed ID: 18543315

The mesopontine rostromedial tegmental nucleus: A structure targeted by the lateral habenula that projects to the ventral tegmental area of Tsai and substantia nigra compacta; Jhou TC, Geisler S, Marinelli M, Degarmo BA, Zahm DS.; J Comp Neurol., 513(6):566-596, 2009, Tested in Rat, PubMed ID: 19235216

Distribution of somatostatin-like immunoreactivity in the brain of the caecilian Dermophis mexicanus (Amphibia: Gymnophiona): comparative aspects in amphibians.; López JM, Moreno N, Morona R, Muñoz M, Domínguez L, González A; J Comp Neurol., 501(3):413-30, 2007, Tested in Amphibian, PubMed ID: 17245705

Developmental Abnormalities of the Neural Structure and Function in Prenatal mice Lacking the Prader-Willi Syndrome Gene Necdin; Pagliardini, Ren, Wevrick, Greer; Neurobiology, 167(1):(1-17), 2005, Tested in Mouse, PubMed ID: 15972963

Central Respiratory Rhythmogenesis Is Abnormal in Lbx1-Deficient Mice; Silvia Pagliardini, Jun Ren, Paul A. Gray, Cassandra VanDunk, Michael Gross, Martyn Goulding, and John J. Greer; Journal of Neuroscience, 28(43):11030 –11041, 2008, Tested in Mouse, PubMed ID: 18945911

Reduced serum concentration is permissive for increased in vitro endocrine differentiation from murine embryonic stem cells; RobertK.Vincent and JonS.Odorico; Differentiation, 78:24–34, 2009, Tested in Mouse, PubMed ID: 19446949

Inhibitory neurons in the anterior piriform cortex of the mouse: classification using molecular markers; Suzuki N, Bekkers JM.; J Comp Neurol., 518(10):1670-87, 2010, Tested in Mouse, PubMed ID: 20235162

MeCP2 is critical within HoxB1-derived tissues of mice for normal lifespan; Christopher S. Ward, E. Melissa Arvide, Teng-Wei Huang, Jong Yoo, Jeffrey L. Noebels, Jeffrey L. Neul; J Neurosci, 31(28):10359 -70, 2011, Tested in Mouse, PubMed ID: 21753013

A novel functionally distinct subtype of striatal neuropeptide Y interneuron; Osvaldo Ibáñez-Sandoval, Fatuel Tecuapetla, Bengi Unal, Fulva Shah, Tibor Koós, James M. Tepper; J Neurosci., 31:16757-69, 2011, Tested in Mouse, PubMed ID: 22090502
20067    Molecular fingerprint of neuropeptide S-producing neurons in the mouse brain; Liu X, Zeng J, Zhou A, Theodorsson E, Fahrenkrug J, Reinscheid RK; J Comp Neurol., 519(10):1847-66, 2011, Tested in Mouse, PubMed ID: 21452230

Cyto- and chemoarchitecture of the hypothalamic paraventricular nucleus in the C57BL/6J male mouse: a study of immunostaining and multiple fluorescent tract tracing; Biag J, Huang Y, Gou L, Hintiryan H, Askarinam A, Hahn JD, Toga AW, Dong HW; J Comp Neurol.,520(1):6-33, 2012, Tested in Mouse, PubMed ID: 21674499

Afferent and efferent connections of the rat retrotrapezoid nucleus; Rosin DL, Chang DA, Guyenet PG.; J Comp Neurol., 122(6):745-752, 1990, Tested in Rat, PubMed ID: 16958085

Ontogeny of the Pre-Botzinger Complex in Perinatal Rats; Silvia Pagliardini, Jun Ren, and John J. Greer; The Journal of Neuroscience, 23(29):9575–9584, 2003, Tested in Rat, PubMed ID: 14573537

Novel Neuronal Phenotypes from Neural Progenitor Cells ; Markakis EA , Palmer TD, Randolph-Moore  L, Rakic P, and Gage FH; The Journal of NeuroScience, 24(12):2886-2897, 2004, Tested in Rat, PubMed ID: 15044527

Differential Localization of the GluR1 and GluR2 Subunits of the AMPA-type Glutamate Receptor Among Striatal Neuron Types in Rats; Y. P. Deng, J. P. Xie, H. B. Wang, W. L. Lei, Q. Chen, and A. Reiner; J Chem Neuroanat. 2007 Jul;33(4):167-92, 2007, Tested in Rat, PubMed ID: 17446041

Immunohistochemical characterization of substance P receptor (NK(1)R)-expressing interneurons in the entorhinal cortex; Wolansky T, Pagliardini S, Greer JJ, Dickson CT.; J Comp Neurol., 502(3):427-41, 2007, Tested in Rat, PubMed ID: 17366610

Neurochemical characterization of tyrosine hydroxylase-immunoreactive interneurons in the developing rat cerebral cortex; Stephen E. Asmus, Emily K. Anderson, Mark W. Ball, Brock A. Barnes, Angela M. Bohnen, Alexander M. Brown, Lucinda J. Hartley, Matthew C. Lally, Tammy M. Lundblad, Joshua B. Martin, Benjamin D. Moss, Kevin D. Phelps, Laura R. Phillips, Cara G. Quilligan, P. Ryan Steed, Shariya L. Terrell, and Ashley E. Warner; Brain Res, 1222:95-105, 2008, Tested in Rat, PubMed ID: 18589406

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Product Reviews

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Reviewed by Dan_Tylee
04/24/2012 - 09:32:00 AM
Strong, Cell-Specific Labeling
Tissue: Maccaca fasciscularis, perfused with saline and 4% paraformaldehyde, dehydrated through increase sucrose gradients, sectioned at 40 um.

Method: Immunocytochemistry on free floating sections. Incubated with ImmunoStar's primary antibody at a concentration of 1:1000 for 4 days in 10% normal goat serum (in 0.1M PO4 buffer with .3% Triton X-100). Rinsed, then incubated with Vector Lab's biotinylated goat anti-rabbit secondary antibody (#BA-1000) for 40 minutes at room temperature in the same 10% normal goat serum solution. Rinsed, then incubated with Vector's standard peroxidase kit (#PK-4000) for 60 minutes at room temperature in 0.1M PO4 buffer with 0.3% Triton X-100. Rinsed, then developed using the instructions for the kit.

Results: Strong, cell-specific labeling. Crisp, punctuate cell bodies. Low background signal. Extremely helpful for delineating the boundaries of the lateral core of the bed nucleus of the stria terminalis (BNST).

Daniel Tylee
Department of Neurobiology & Anatomy
University of Rochester - School of Medicine and Dentistry