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Somatostatin 28 Antibody

Somatostatin 28 Antibody
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Price: $250.00
Product ID : 20089
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Description

The antibody has a proven Biotin-Streptavidin/HRP staining at a 1/1000-1/2000 dilution in rat hypothalamus/median eminence.

Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg/mL of Somatostatin 28 (Tyr1-14).

Photo Description: IHC image of the median eminence staining for somatostatin-28 with standard biotin-streptavidin/HRP and DAB chromogen (above) and FITC-conjugated secondary antibody (below). The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS.

Somatostatin 28 #20089

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Rat

Availability: In Stock

Alternate Names:   Growth hormone release-inhibiting factor 28; SMST; somatostatin, (3C11 in reference # 2345961)

Gene Symbol / ID, Accession #:   SST,6750

 

Download Product Spec. SheetSomatostatin 28 Antibody

 

Journal References:

Reciprocal connectivity between mitral cells and external plexiform layer interneurons in the mouse olfactory bulb, Longwen Huang, Isabella Garcia, Hsin-I Jen, and Benjamin R. Arenkiel, Front Neural Circuits, 7:32, 2013, Tested in Mouse, PubMed ID: 2345961

A group of glutamatergic interneurons expressing high levels of both neurokinin-1 receptors and somatostatin identifies the region of the pre-Botzinger complex.; Stornetta RL, Rosin DL, Wang H, Sevigny CP, Weston MC, Guyenet PG; J Comp Neurol, 455(4):499-512, 2002, Tested in Rat, PubMed ID: 12508323



Curia, Giulia, et al. "Resilience to audiogenic seizures is associated with p-ERK1/2 dephosphorylation in the subiculum of Fmr1 knockout mice." Frontiers in cellular neuroscience 7 (2013). Tested in Mouse, PubMed ID: 23630463

Rose, Matthew F., et al. "Math1 Is Essential for the Development of Hindbrain Neurons Critical for Perinatal Breathing." Neuron 64.3 (2009): 341-354. Tested in Mouse, PubMed ID: 19914183

Pinho, M. S., and S. Gulbenkian. "Innervation of the canine mammary gland: an immunohistochemical study." (2007). PubMed ID: 17647190

Straessle, Andrea, et al. "Rapid and long‐term alterations of hippocampal GABAB receptors in a mouse model of temporal lobe epilepsy." European Journal of Neuroscience 18.8 (2003): 2213-2226. Tested in Mouse, PubMed ID: 14622182

Drake, Carrie T., and Teresa A. Milner. "Mu opioid receptors are in discrete hippocampal interneuron subpopulations." Hippocampus 12.2 (2002): 119-136. PubMed ID: 12000113

Mura, A., et al. "Striatal cells containing aromatic L-amino acid decarboxylase: an immunohistochemical comparison with other classes of striatal neurons." Neuroscience 98.3 (2000): 501-511. PubMed ID: 10869844

Huang, Jianhua, and Mark L. Weiss. "Characterization of the central cell groups regulating the kidney in the rat." Brain research 845.1 (1999): 77-91. Tested in Rat, PubMed ID: 10529446

Zhang, Zhang-Jin, et al. "Selective lesion of the cholinergic basal forebrain causes a loss of cortical neuropeptide Y and somatostatin neurons." Brain research 800.2 (1998): 198-206. PubMed ID: 9685641

Nomura, Takuo, et al. "Distribution of nonprincipal neurons in the rat hippocampus, with special reference to their dorsoventral difference." Brain research 751.1 (1997): 64-80.  Tested in Rat, PubMed ID: 9098569

Nomura, Takuo, et al. "Laminar distribution of non-principal neurons in the rat hippocampus, with special reference to their compositional difference among layers." Brain research 764.1 (1997): 197-204. Tested in Rat, PubMed ID: 9295210

Crick, S. J., et al. "A quantitative study of nerve distribution in the conduction system of the guinea pig heart." Journal of anatomy 188.Pt 2 (1996): 403. Tested in Guinea Pig, PubMed ID: 8621340

Commons, Kathryn G., and Teresa A. Milner. "Cellular and subcellular localization of δ opioid receptor immunoreactivity in the rat dentate gyrus." Brain research 738.2 (1996): 181-195. PubMed ID: 8955512

Barajon, Isabella, et al. "Neuropeptides and morphological changes in cisplatin-induced dorsal root ganglion neuronopathy." Experimental neurology 138.1 (1996): 93-104. PubMed ID: 8593900

Mathern, Gary W., et al. "Reactive synaptogenesis and neuron densities for neuropeptide Y, somatostatin, and glutamate decarboxylase immunoreactivity in the epileptogenic human fascia dentata." The Journal of neuroscience 15.5 (1995): 3990-4004. Tested in Human, PubMed ID: 7751960

Crick, Simon J., et al. "Innervation of the human cardiac conduction system. A quantitative immunohistochemical and histochemical study." Circulation 89.4 (1994): 1697-1708. PubMed ID: 10580850

Quartu, M., J. M. Polak, and M. Del Fiacco. "Neuropeptides in the human celiac/superior mesenteric ganglionic complex: an immunohistochemical study." Journal of chemical neuroanatomy 6.2 (1993): 79-99. Tested in Human, PubMed ID: 8476542

Quartu, M., et al. "Immunohistochemical Localization of Putative Peptide Neurotransmitters in the Human Trigeminal Sensory Systema." Annals of the New York Academy of Sciences 657.1 (1992): 469-472. Tested in Human, PubMed ID: 1379018

Casasco, A., et al. "Immunocytochemical evidence for the presence of somatostatin-like immunoreactive nerves in human dental pulp." Journal of dental research 70.2 (1991): 87-89. Tested in Human, PubMed ID: 1671397

Davies, C. A., et al. "A quantitative assessment of somatostatin-like and neuropeptide Y-like immunostained cells in the frontal and temporal cortex of patients with Alzheimer's disease." Journal of the neurological sciences 96.1 (1990): 59-73. PubMed ID: 1972185

Kita, H., and S. T. Kitai. "Amygdaloid projections to the frontal cortex and the striatum in the rat." Journal of Comparative Neurology 298.1 (1990): 40-49. Tested in Rat, PubMed ID: 1698828

Caserta, Maria T., et al. "Increased number of somatostatin-immunoreactive neurons in primary cultures of trisomy 16 mouse neocortex." Molecular Brain Research 7.3 (1990): 269-272. PubMed ID: 1970846

Aramant, Robert, et al. "Neuronal markers in rat retinal grafts." Developmental Brain Research 53.1 (1990): 47-61. Tested in Rat, PubMed ID: 1972041

Casasco, A., et al. "Peptidergic nerves in human dental pulp." Histochemistry 95.2 (1990): 115-121. Tested in Human, PubMed ID: 2081689

Takami, Shigeru, Misbah H. El-Hawary, and Pasquale PC Graziadei. "Somatostatin-28-like immunoreactivity in the rat olfactory bulb." Brain research 526.2 (1990): 333-337. Tested in Rat, PubMed ID: 1979520

Milner, Teresa A., and Catherine E. Bacon. "Ultrastructural localization of somatostatin‐like immunoreactivity in the rat dentate gyrus." Journal of Comparative Neurology 290.4 (1989): 544-560.

Wharton, J., et al. "Immunohistochemical and ultrastructural localisation of peptide-containing nerves and myocardial cells in the human atrial appendage." Cell and tissue research 254.1 (1988): 155-166. Tested in Human, PubMed ID: 2973836

Kunkel, Dennis D., and Philip A. Schwartzkroin. "Ultrastructural characterization and GAD Co‐localization of somatostatin‐like immunoreactive neurons in CA1 of rabbit hippocampus." Synapse 2.4 (1988): 371-381. PubMed ID: 2903565

Bachoo, Manjit, John Ciriello, and Canio Polosa. "Effect of preganglionic stimulation on neuropeptide-like immunoreactivity in the stellate ganglion of the cat." Brain research 400.2 (1987): 377-382. Tested in Cat, PubMed ID: 3815084

Köhler, Christer, Lena Haglund, and Larry W. Swanson. "A diffuse αMSH‐immunoreactive projection to the hippocampus and spinal cord from individual neurons in the lateral hypothalamic area and zona incerta." Journal of Comparative Neurology 223.4 (1984): 501-514. PubMed ID: 6325509

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Product Reviews

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Reviewed by isaacson
06/05/2013 - 02:52:50 PM
SOM 28 antibody
I tested the SOM 28 antibody on 50um piriform cortex sections from mice fix-perfused but NOT cryopreserved. Staining and signal to noise were very good using a 1:500 dilution of the antibody in combination iwth 5% normal goat serum and 0.3% TX100 in PBS and O/N incubation at 4%. Immunostaining was visualized using a 1:500 GAR-Alexa 488 secondary anbibody.