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Parvalbumin Antibody

Parvalbumin Antibody
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  • Parvalbumin (RED) interneurons pictured in mouse striatum, GREEN is GFP expressed by medium spiny neurons.
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Price: $350.00
Product ID : 24428
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Description

The antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat thalamus, cortex, and hippocampus.

The antiserum has been characterized as specific to parvalbumin; please see reference listed below. Recommended primary dilutions are 1/400 - 1/800 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/5,000 - 1/8,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique.

Photo Description: IHC image of the rat hippocampus staining for parvalbumin. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

The second image above entitled "D." is parvalbumin (RED) interneurons pictured in mouse striatum, GREEN is GFP expressed by medium spiny neurons, provided Alipi Naydenov, Neurobiology and Behavior, Stella Lab University of Washington (see review below).

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Human, Mouse, Pigeon, Rat

Availability: In Stock

Alternate Names:  Parvalbumin alpha; Pva; Parvalbumin (calcium binding protein); PALB1, PC255L (Millipore Cat #)

Gene Symbol / ID, Accession #:  Pvalb, 25269

Download Product Spec. SheetParvalbumin Antibody

 

Journal References:


Ünal, Bengi, et al. "Anatomical and electrophysiological changes in striatal TH interneurons after loss of the nigrostriatal dopaminergic pathway." Brain Structure and Function (2013): 1-19. PubMed ID: 24173616

Horne, Eric A., et al. "Downregulation of cannabinoid receptor 1 from neuropeptide Y interneurons in the basal ganglia of patients with Huntington's disease and mouse models." European Journal of Neuroscience 37.3 (2013): 429-440. Tested in Mouse and Human, PubMed ID: 23167744

Murakami, K., Y. Ishikawa, and F. Sato. "Localization of α7 nicotinic acetylcholine receptor immunoreactivity on GABAergic interneurons in layers I–III of the rat retrosplenial granular cortex." Neuroscience 252 (2013): 443-459. PubMed ID: 23985568

Kuang, Yi, et al. "Dicer1 and MiR‐9 are required for proper Notch1 signaling and the Bergmann glial phenotype in the developing mouse cerebellum." Glia 60.11 (2012): 1734-1746. Tested in Mouse, PubMed ID: 22836445

Sherry M. Zakhary, Diana Ayubcha, Jeffery N. Dileo, Riya Jose, Joerg R. Leheste, Judith M. Horowitz, And German Torres; "Distribution Analysis of Deacetylase SIRT1 in Rodent and Human Nervous Systems"; THE ANATOMICAL RECORD,293:1024-1032 (2010). Tested in Human, Mouse, Rat, PubMed ID: 20225204

Ibáñez-Sandoval, Osvaldo, et al. "Electrophysiological and morphological characteristics and synaptic connectivity of tyrosine hydroxylase-expressing neurons in adult mouse striatum." The Journal of Neuroscience 30.20 (2010): 6999-7016. Tested in Mouse,    PubMed ID: 20484642

Briatore, Federica, et al. "Quantitative organization of GABAergic synapses in the molecular layer of the mouse cerebellar cortex." PloS one 5.8 (2010): e12119. Tested in Mouse, PubMed ID: 20711348

Browning, Philip GF, et al. "Severe scene learning impairment, but intact recognition memory, after cholinergic depletion of inferotemporal cortex followed by fornix transection." Cerebral Cortex 20.2 (2010): 282-293. Tested in Monkey, PubMed ID: 19447862

Deng, Y. P., et al. "Differential localization of the GluR1 and GluR2 subunits of the AMPA-type glutamate receptor among striatal neuron types in rats." Journal of chemical neuroanatomy 33.4 (2007): 167-192. Tested in Rat, PubMed ID: 17446041

Schneider Gasser, Edith M., et al. "Reorganization of GABAergic circuits maintains GABAA receptor‐mediated transmission onto CA1 interneurons in α1‐subunit‐null mice." European Journal of Neuroscience 25.11 (2007): 3287-3304. Tested in Mouse, PubMed ID: 17552997

Antonio V. Laverghetta, Claudio A.B. Toledo, C. Leo Veenman, Kei Yamamoto, Hongbing Wang, Anton Reiner; Cellular Localization of AMPA Type Glutamate Receptor Subunits in the Basal Ganglia of Pigeons (Columba livia); Brain Behav Evol,67:10–38. Tested in Pigeon, PubMed ID: 16219996

Blurton‐Jones, Mathew, and Mark H. Tuszynski. "Estradiol‐induced modulation of estrogen receptor‐β and GABA within the adult neocortex: A potential transsynaptic mechanism for estrogen modulation of BDNF." Journal of Comparative Neurology 499.4 (2006): 603-612. Tested in Rat, PubMed ID: 17029253

Arabadzisz, Dimitrula, et al. "Epileptogenesis and chronic seizures in a mouse model of temporal lobe epilepsy are associated with distinct EEG patterns and selective neurochemical alterations in the contralateral hippocampus." Experimental neurology 194.1 (2005): 76-90. Tested in Mouse, PubMed ID: 15899245

André, Véronique, et al. "Alterations of hippocampal GABAergic system contribute to development of spontaneous recurrent seizures in the rat lithium‐pilocarpine model of temporal lobe epilepsy." Hippocampus 11.4 (2001): 452-468. Tested in Rat, PubMed ID: 11530850

Bäckman, Cristina, et al. "Cellular mRNA expression of the transcription factor NGFI-B suggests a gene regulatory role in striatal opiate-peptide neurons." Brain research 903.1 (2001): 26-32. PubMed ID: 11382384

Sarabi, Arezou, et al. "GFRα‐1 mRNA in dopaminergic and nondopaminergic neurons in the substantia nigra and ventral tegmental area." Journal of Comparative Neurology 441.2 (2001): 106-117. Tested in Rat, PubMed ID: 11745638

Sarabi, Arezou, et al. "GFRα-1 is expressed in parvalbumin GABAergic neurons in the hippocampus." Brain research 877.2 (2000): 262-270. PubMed ID: 10986340

Weiss, John H., et al. "Cortical neurons containing somatostatin‐or parvalbumin‐like immunoreactivity are atypically vulnerable to excitotoxic injury in vitro." Neurology 40.8 (1990): 1288-1288. PubMed ID: 1974332

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Product Reviews

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Reviewed by user
11/15/2012 - 02:23:41 PM
Antibody Performed Very Well
The second image above labeled "D." is parvalbumin (RED) interneurons pictured in mouse striatum, GREEN is GFP expressed by medium spiny neurons. Parvalbumin was used at 1:1000. This antibody performed very well. Scale bar = 25 microns.

Immunohistochemistry: Mice were euthanized and perfused with PFA (4% in PBS), post fixed for 24 hrs, and their brains cryoprotected in 15% sucrose (24 hrs) followed by 30% sucrose (48 hrs). Coronal sections that included the corticostriatal, globus pallidus, or substantia nigra regions (30 um) were prepared using a microtome and then stored in PBS at 4C until processing. Sections were processed/stained in parallel as follows: Free floating sections were rinsed 3x with PBS and incubated 90 min at room temperature (RT) in PBS supplemented with donkey serum (5%) and Triton X-100 (1%). Primary antibody combinations were prepared as a master stock in PBS supplemented with donkey serum (2.5%) and Triton X-100 (0.5%) and applied to sections for 72 hrs at 4oC with gentle agitation. Sections were then rinsed 8x with PBS supplemented with Tween-20 (0.05%, at RT) and incubated with a secondary antibodies diluted in PBS supplemented with donkey serum (2.5%) and Triton X-100 (0.5%) for 1 hr at RT with gentle agitation, followed by 7 rinses with PBS and one rinse with deionized water. Sections were mounted onto slides and allowed to dry for ~18 hrs, after which cover slips were mounted with Vectashield and sealed with nail polish.

Review submitted by:

Alipi Naydenov
Neurobiology and Behavior, Stella Lab
University of Washington

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