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Parvalbumin Antibody

Parvalbumin Antibody
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  • Parvalbumin (RED) interneurons pictured in mouse striatum, GREEN is GFP expressed by medium spiny neurons.
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Price: $350.00
Product ID : 24428
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Description

The antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat thalamus, cortex, and hippocampus.

The second image above entitled "D." is parvalbumin (RED) interneurons pictured in mouse striatum, GREEN is GFP expressed by medium spiny neurons, provided Alipi Naydenov, Neurobiology and Behavior, Stella Lab University of Washington (see review below).

The antiserum has been characterized as specific to parvalbumin; please see reference listed below. Recommended primary dilutions are 1/400 - 1/800 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/5,000 - 1/8,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique.

Slide of Rat Cortex using IHC at 100x:
Rat Cortex at 100x


Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Human, Mouse, Pigeon, Rat

Availability: In Stock

Alternate Names:  Parvalbumin alpha; Pva; Parvalbumin (calcium binding protein); PALB1, PC255L (Millipore Cat #)

Gene Symbol / ID, Accession #:  Pvalb, 25269

Download Product Spec. SheetParvalbumin Antibody


Journal References:


Distribution Analysis of Deacetylase SIRT1 in Rodent and Human Nervous Systems; Sherry M. Zakhary, Diana Ayubcha, Jeffery N. Dileo, Riya Jose, Joerg R. Leheste, Judith M. Horowitz, And German Torres; THE ANATOMICAL RECORD, 293:1024-1032, 2010, Tested in Human, PubMed ID: 20225204

Cellular Localization of AMPA Type Glutamate Receptor Subunits in the Basal Ganglia of Pigeons (Columba livia); Antonio V. Laverghetta, Claudio A.B. Toledo, C. Leo Veenman, Kei Yamamoto, Hongbing Wang, Anton Reiner ; Brain Behav Evol, 67:10–38, 2006, Tested in Pigeon, PubMed ID: 16219996

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Product Reviews

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Reviewed by user
11/15/2012 - 02:23:41 PM
Antibody Performed Very Well
The second image above labeled "D." is parvalbumin (RED) interneurons pictured in mouse striatum, GREEN is GFP expressed by medium spiny neurons. Parvalbumin was used at 1:1000. This antibody performed very well. Scale bar = 25 microns.

Immunohistochemistry: Mice were euthanized and perfused with PFA (4% in PBS), post fixed for 24 hrs, and their brains cryoprotected in 15% sucrose (24 hrs) followed by 30% sucrose (48 hrs). Coronal sections that included the corticostriatal, globus pallidus, or substantia nigra regions (30 um) were prepared using a microtome and then stored in PBS at 4C until processing. Sections were processed/stained in parallel as follows: Free floating sections were rinsed 3x with PBS and incubated 90 min at room temperature (RT) in PBS supplemented with donkey serum (5%) and Triton X-100 (1%). Primary antibody combinations were prepared as a master stock in PBS supplemented with donkey serum (2.5%) and Triton X-100 (0.5%) and applied to sections for 72 hrs at 4oC with gentle agitation. Sections were then rinsed 8x with PBS supplemented with Tween-20 (0.05%, at RT) and incubated with a secondary antibodies diluted in PBS supplemented with donkey serum (2.5%) and Triton X-100 (0.5%) for 1 hr at RT with gentle agitation, followed by 7 rinses with PBS and one rinse with deionized water. Sections were mounted onto slides and allowed to dry for ~18 hrs, after which cover slips were mounted with Vectashield and sealed with nail polish.

Review submitted by:

Alipi Naydenov
Neurobiology and Behavior, Stella Lab
University of Washington

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