nNOS:N-Terminal (Neuronal Nitric Oxide Synthase) Antibody

The ImmunoStar N-terminal neuronal nitric oxide synthase antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000 - 1/2,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP.

By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immunolabeling is completely abolished by pre-adsorption with synthetic human nNOS (134-148) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS was observed.

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With:  Mouse, Rat

Availability: In Stock

Alternate Names:  Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; Nitric Oxide Synthase; bNOS; Nitric oxide synthase-brain; N-NOS; Peptidyl-cysteine S-nitrosylase; IHPS1; NC-NOS; nitric oxide synthase 1 (neuronal)   

Gene Symbol / ID, Accession #:  NOS1, 4842

Download Product Spec. Sheet:  nNOS:N-Terminal Antibody

Journal References:

Developmentally regulated and evolutionarily conserved expression of SLITRK1 in brain circuits implicated in Tourette syndrome; ; Journal of comparative neurology,513(1):21-37, 2009, Tested in Mouse, Western Blot, PubMed ID: 19105198

Biglycan regulates the expression and sarcolemmal localization of dystrobrevin, syntrophin, and nNOS; Mercado ML, Amenta AR, Hagiwara H, Rafii MS, Lechner BE, Owens RT, McQuillan DJ, Froehner SC, Fallon JR.; FASEB J.,20 (10):1724-26, 2006, Tested in Mouse, Western Blot, IFC, PubMed ID: 16807372

Biglycan is an extracellular MuSK binding protein important for synapse stability; A.R. Amenta, H.E. Creely, M.L. Mercado, H. Hagiwara, B. A. McKechnie, B.E.Lechner, S. G. Rossi, Q. Wang, R. T. Owens, E. Marrero, L. Mei, W. Hoch, M. F.Young, D. J. McQuillan, R. L. Rotundo, and J.R. Fallon; J Neurosci.,32(7):2324-2334, 2012, Tested in Mouse, PubMed ID: 22396407

Confocal immunofluorescence study of rat aortic body chemoreceptors and associated neurons in situ and in vitro; Nikol A. Piskuric, Cathy Vollmer, Colin A. Nurse; The Journal of Comparative Neurology,519(5):856-73, 2011, Tested in Rat, PubMed ID: 21280041