nNOS:C-Terminal (Neuronal Nitric Oxide Synthase)

The ImmunoStar neuronal nitric oxide synthase C-terminal antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000 - 1/1,500 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/8,000 - 1/12,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique.

By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immuno-labeling is completely abolished by pre-adsorption with synthetic human nNOS (1419-1433) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS were observed. The nNOS antiserum has been used successfully in human, rat, mouse, guinea pig, cat, and monkey tissue. Detection of nNOS from other species will depend upon sequence homology.

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Hamster, Mouse, Rat

Predicted to React With: Cat, Guinea Pig, Human, Monkey

Availability: In Stock

Alternate Names:  Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; Nitric Oxide Synthase; bNOS; Nitric oxide synthase-brain; N-NOS; Peptidyl-cysteine S-nitrosylase; IHPS1; NC-NOS; nitric oxide synthase 1 (neuronal)

Gene Symbol / ID, Accession #:  NOS1, 4842

Download Product Spec. Sheet:  nNOS:C-Terminal Antibody

Journal References:

Innervation of the rat uterus at estrus: a study in full-thickness, immunoperoxidase-stained whole-mount preparations; Gnanamanickam GJ, Llewellyn-Smith IJ; J Comp Neurol., 519(4):621-43, 2011, Tested in Rat, Cites Diasorin AKA ImmunoStar, PubMed ID: 21246547

Neuronal Nitric Oxide Synthase Activation and Peroxynitrite Formation in Ischemic Stroke Linked to Neural Damage; Eliasson MJL, Huang Z, Ferrante RJ; The Journal of Neuroscience,19(14):5910-5918, 1999, Tested in Mouse, Cites Incstar AKA ImmunoStar, PubMed ID: 10407030

Rapid changes on nitrinergic system in female mouse hippocampus during the ovarian cycle; Stefano Gotti, Mariangela Martini, Monica Pradotto, Carla Viglietti-Panzica, GianCarlo Panzica; Journal of Chemical Neuroanatomy, 38:117–123, 2009, Tested in Mouse, Cites Incstar AKA ImmunoStar, PubMed ID: 19559786

Neuronal nitric oxide synthase alternatively spliced forms:  Prominent functional localizations in the brain; Mikael J. L. Eliasson, Seth Blackshaw,  Michael J. Schell, And Solomon H. Snyder; Proc. Natl. Acad. Sci. USA, Vol. 94:pp. 3396–3401, 1997, Tested in Mouse, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 9096405

The Mitochondrial Permeability Transition Pore Regulates Nitric Oxide-Mediated Apoptosis of Neurons Induced by Target Deprivation; Lee J. Martin, Neal A. Adams, Yan Pan, Ann Price, Margaret Wong; J Neurosci,31(1):359 -70, 2011, Tested in Mouse, Western Blot, PubMed ID: 21209222

Photoperiod Affects Neuronal Nitric Oxide synthase and Aggressive Behaviour in Male Siberian Hamsters (Phodopus sungorus); Wen JC, Hotchkiss AK, Demast GE, Nelson RJ; Journal of Neuroendocrinology, 16:916-921, 2004, Tested in Hamster, PubMed ID: 15584932

Distribution of Nitric Oxide Synthase Immunoreactivity in the Mouse Brain; Gotti, Sica, Viglietti-Panzica, Panzica; Microscopy Research and Technique, 68:13-35, 2005, Tested in Mouse, PubMed ID: 16208717

Impaired Nitric Oxide Synthase Signaling Dissociates Social Investigation and Aggression; Brian C. Trainor, Joanna L. Workman, Ruth Jessen, and Randy J. Nelson; Behav Neurosci, 121:362-369, 2007, Tested in Mouse, PubMed ID: 17469926

Sex differences in brain developing in the presence or absence of gonads; Tomaz Büdefeld, Neza Grgurevic, Stuart A. Tobet, and Gregor Majdic; Dev Neurobiol., 68(7):981-995, 2008, Tested in Mouse, PubMed ID: 18418875

Roles for c-Aminobutyric Acid in the Development of
the Paraventricular Nucleus of the Hypothalamus; ; J Comp Neurol, 518(14):2710-28, 2010, Tested in Mouse, PubMed ID: 20506472

Lis1 reduction causes tangential migratory errors in mouse spinal cord; Moore KD, Chen R, Cilluffo M, Golden JA, Phelps PE; J Comp Neurol., 520(6):1198-211, 2012, Tested in Mouse, PubMed ID: 21935943

Tyrosine-phosphorylated and nonphosphorylated isoforms of alpha-dystrobrevin: roles in skeletal muscle and its neuromuscular and myotendinous junctions; R. Mark Grady, Mohammed Akaaboune, Alexander L. Cohen, Margaret M. Maimone, Jeff W. Lichtman, and Joshua R. Sanes; The Journal of Cell Biology, 160:741-752, 2003, Tested in Mouse, PubMed ID: 12604589

Layer Acquisition by Cortical GABAergic Interneurons Is Independent of Reelin Signaling; Ramon Pla, Vıctor Borrell, Nuria Flames, and Oscar Marın; Journal of Neuroscience, 26(26):6924–6934, 2006, Tested in Mouse, PubMed ID: 16807322

Motor neuron degeneration in amyotrophic lateral sclerosis mutant superoxide dismutase-1 transgenic mice: mechanisms of mitochondriopathy and cell death; Martin LJ, Liu Z, Chen K, Price AC, Pan Y, Swaby JA, Golden WC.; J Comp Neurol., 500(1):20-46, 2007, Tested in Mouse, PubMed ID: 17099894

Estrogen modulates neuronal movements within the developing preoptic area/anterior hypothalamus; John Gabriel Knoll, Cory A. Wolfe, and Stuart A. Tobet; Eur J Neurosci., 26(5):1091-1100, 2008, Tested in Mouse, PubMed ID: 17767488

A novel O2-sensing mechanism in rat glossopharyngeal neurones mediated by a halothane-inhibitable background K+ conductance; Verónica A. Campanucci, Ian M. Fearon and Colin A. Nurse; J Physiol, 548.3:731-743, 2003, Tested in Rat, PubMed ID: 12640017

Taste-evoked Fos expression in nitrergic neurons in the nucleus of the solitary tract and reticular formation of the rat; Travers SP, Travers JB.; J Comp Neurol., 500(4):746-60, 2007, Tested in Rat, PubMed ID: 17154256

Variability in the occurrence of nitric oxide synthase immunoreactivity in different populations of rat sympathetic preganglionic neurons; Hinrichs JM, Llewellyn-Smith IJ.; J Comp Neurol., 514(5):492-506, 2009, Tested in Rat, PubMed ID: 19350665