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nNOS:C-Terminal (Neuronal Nitric Oxide Synthase)

nNOS:C-Terminal (Neuronal Nitric Oxide Synthase)
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Price: $310.00
Product ID : 24287
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Description

The ImmunoStar neuronal nitric oxide synthase C-terminal antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/8,000 - 1/12,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique.

By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immuno-labeling is completely abolished by pre-adsorption with synthetic human nNOS (1419-1433) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS were observed. 

Photo Description: IHC image of a single neuron staining for the C-terminal of nNOS in the rat cortex (above), along with low magnification of cortex (below left), neurons in the rat dentate gyrus (below middle) and in the striatum (below right). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen, below middle image with nickel stain.

 nNos C-Terminal cortex #24287    nNOS-C-terminal striatum #24287


Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Hamster, Mouse, Rat

Availability: In Stock

Alternate Names:  Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; Nitric Oxide Synthase; bNOS; Nitric oxide synthase-brain; N-NOS; Peptidyl-cysteine S-nitrosylase; IHPS1; NC-NOS; nitric oxide synthase 1 (neuronal)

Gene Symbol / ID, Accession #:  NOS1, 4842

 

Download Product Spec. SheetnNOS:C-Terminal Antibody

 

Journal References:


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