Neurotensin Antibody

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Price: $250.00

Product ID : 20072

2 review(s)

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Description

The ImmunoStar Neurotensin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat amygdala using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/200-1/400 in PBS/0.3% Triton x-100 - Cy3 Technique and 1/4000-1/8000 in PBS/0.3% Triton x-100 - Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment with 10 µg of Neurotensin per 1 mL of diluted antibody.

Host: Rabbit

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Reacts With: Cat, Human, Mouse, Rat

Availability: In Stock

Alternate Names: neuromedin N; Preproneurotensin; pro-neurotensin

Gene Symbol / ID, Accession #: NTS, 4922

Download Product Spec. Sheet: Neurotensin Antibody

Journal References:

Immunohistochemical localization of putative neurotransmitters within the feline nucleus tractus solitarii; B. Maley, R. Elde; Neuroscience,7(10):2469–2490, 1982, Tested in Cat, IFC, Cites ImmunoNuclear AKA ImmunoStar, PubMed ID: 6184644

Neurotensin-immunoreactive neurons in the ventral striatum of the adult rat : ventromedial caudate-putamen, nucleus accumbens and olfactory tubercle; Zahm DS.; Neuroscience letters,81(1-2):41-7, 1987, Tested in Rat, Cites ImmunoNuclear AKA ImmunoStar, PubMed ID: 3696473

Regional Distribution, Characterization, and Inactivation; Emson PC, Goedert M, Williams B, Ninkovic M, Hunt SP.; Ann N Y Acad Sci.,400:198-215, 1982, Tested in Rat, IFC, Cites ImmunoNuclear AKA ImmunoStar, PubMed ID: 6762831

Suprachiasmatic nucleus organization.; Moore RY, Speh JC, and Leak RK; Cell Tissue Res,309:89-98, 2002, Tested in Rat, Cites Incstar AKA ImmunoStar, PubMed ID: 12111539

Plasma molecular forms of gastrin, neurotensin and somatostatin in pregnancy and gestational diabetes after an oral glucose load or a mixed meal; Marcos Martinez , Angel Hernanz , Cristina Grande and Luis Felipe Pallardo; Regulatory Peptides,,47:73-80, 1993, Tested in Human , PubMed ID: 8105515

Anorexigenic Hormones Leptin, Insulin, and {alpha}-Melanocyte-Stimulating Hormone Directly Induce Neurotensin (NT) Gene Expression in Novel NT-Expressing Cell Models ; Cui, Cai, Belsham ; The Journal of Neuroscience,25(41):9497-9506, 2005, Tested in Mouse, PubMed ID: 16221860

Inputs to the Midbrain Dopaminergic Complex in the Rat, with Emphasis on Extended Amygdala-Recipient Sectors; Daniel S. Zahm, Anita Y. Cheng, Tristan J. Lee, Comeron W. Ghobadi, Zachary M. Schwartz, Stefanie Geisler, Kenneth P. Parsely, Clemens Gruber, and Ruediger W. Veh; The Journal of Comparative Neurology,519(16):3159-3188, 2011, Tested in Rat, PubMed ID: 21618227

Gfi1 functions downstream of Math1 to control intestinal secretory cell subtype allocation and differentiation; Shroyer, Wallis,Venken ,Bellen, Zoghbi ; Genes & Development,19:2412-2417, 2005, Tested in Mouse, PubMed ID: 16230531

Distribution and neurochemical characterization of neurons within the nucleus of the solitary tract responsive to serotonin agonist-induced hypophagia; Daniel D. Lama, Ligang Zhoub, Andreas Veggea,c, Philip Y. Xiua, Britt T. Christensena,C, Mayowa A. Osundijid, Chen-yu Yuehd,E, Mark L. Evansd, and Lora K. Heislera; Behav Brain Res,196:139-143, 2009, Tested in Rat, PubMed ID: 18762217

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Product Reviews

Reviewed by droot
07/10/2012 - 10:12:41 PM

Visualization of ventromedial ventral pallidum

For our manuscript, Differential roles of ventral pallidum subregions during cocaine self-administration behaviors, in press at Journal of Comparative Neurology, we used the Immunostar rabbit anti-neurotensin primary antibody to visualize the ventromedial ventral pallidum subregion.

Rats were perfused with saline followed by 4% PF, stored in 30% sucrose, and coronally sectioned to 40 um. We followed the protocols of Zahm/Heimer and colleagues when they discovered the ventral pallidum subregions and their afferent/efferent projection patterns.

The protocol consisted of the following steps:
1. Wash in 0.1M phosphate buffer (PB; pH 7.4)
2. 15 min in 1% sodium borohydride
3. Wash
4. 1 hour blocking with PB containing 0.1% Triton X-100 and 3% normal goat serum
5. Primary antibody overnight at 4C - ImmunoStar rabbit anti-neurotensin diluted 1 : 6500 in PB containing 0.1% Triton X-100 and 3% normal goal serum
6. Wash
7. 1 hour anti-rabbit biotinylated secondary (Vector) 1:200 in PB with 0.1% Triton X-100
8. Wash
9. 1 hour ABC (Vector) 1:200 in PB with 0.1% Triton X-100
10. Wash
11. Develop with 0.05% DAB for 6 min

Our study involved recording neurons within the ventral pallidum subregions during specific aspects of intravenous cocaine self-administration (approaching toward, responding on, or retreating away from a cocaine-reinforced operandum). In order to verify the placement of microwires within the VP subregions, prior to perfusion we passed current through each stainless steel microwire to leave an iron deposit at the uninsulated tip. After DAB (brown reaction) and mounting, we visualized the iron deposit by incubating in a 5% potassium ferrocyanide and 10% HCl solution (leaving a blue-green reaction).

For our purposes, this antibody labeled fibers in ventromedial VP with low background. Individual neurons and other fibers were clearly observed within BNST that were not involved in our study.

I rated this antibody 4 stars because there was some variability for this stain between animals.

David H. Root, Ph.D.
Rutgers University

Reviewed by Dan_Tylee
04/05/2012 - 01:09:25 PM

Crisp, Cell-specific labeling

Tissue: Maccaca fasciscularis, perfused with saline and 4% paraformaldehyde, dehydrated through increase sucrose gradients, sectioned at 40 um with sliding microtome.

Method: Immunocytochemistry on free floating sections. Incubated with ImmunoStar's primary antibody at a concentration of 1:1000 for 4 days in 10% normal goat serum (in 0.1M PO4 buffer with .3% Triton X-100). Rinsed, then incubated with Vector Lab's biotinylated goat anti-rabbit secondary antibody (#BA-1000) at a concentration of 1:200 for 40 minutes at room temperature in the same 10% normal goat serum solution. Rinsed, then incubated with Vector's standard peroxidase kit (#PK-4000) for 60 minutes at room temperature in 0.1M PO4 buffer with .3% Triton X-100. Rinsed, then developed using the instructions for the kit.

Results: Crisp, cell-specific labeling. Low background. Very helpful for determining the boundary between the medial and lateral core divisions of the central nucleus of the amygdala.

Daniel Tylee
Department of Neurobiology & Anatomy
University of Rochester - School of Medicine and Dentistry