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Neurotensin Antibody

Neurotensin Antibody
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Price: $250.00
Product ID : 20072
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Description

The ImmunoStar Neurotensin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat amygdala using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/4000-1/8000 in PBS/0.3% Triton x-100 - Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment with 10 µg of Neurotensin per 1 mL of diluted antibody.

Photo Description: IHC image of rat amygdala staining for neurotensin. The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. 

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Cat, Human, Mouse, Rat

Availability: In Stock

Alternate Names:  neuromedin N; Preproneurotensin; pro-neurotensin

Gene Symbol / ID, Accession #:  NTS, 4922

 

Download Product Spec. SheetNeurotensin Antibody

 

Journal References:


deCampo, Danielle M., and Julie L. Fudge. "Amygdala projections to the lateral bed nucleus of the stria terminalis in the macaque: Comparison with ventral striatal afferents." Journal of Comparative Neurology 521.14 (2013): 3191-3216. Tested in Monkey, PubMed ID: 23696521

Meister, B., M. Perez‐Manso, and T. Daraio. "Delta‐like 1 Homologue is a Hypothalamus‐Enriched Protein that is Present in Orexin‐Containing Neurones of the Lateral Hypothalamic Area." Journal of neuroendocrinology 25.7 (2013): 617-625. Tested in Rat, PubMed ID: 23387476

Root, David H., et al. "Differential roles of ventral pallidum subregions during cocaine self‐administration behaviors." Journal of Comparative Neurology 521.3 (2013): 558-588. Tested in Rat, PubMed ID: 22806483

Laque, Amanda, et al. "Leptin receptor neurons in the mouse hypothalamus are colocalized with the neuropeptide galanin and mediate anorexigenic leptin action." American Journal of Physiology-Endocrinology and Metabolism 304.9 (2013): E999-E1011. Tested in Mouse, PubMed ID:     23482448

Mumphrey, M. B., et al. "Roux‐en‐Y gastric bypass surgery increases number but not density of CCK‐, GLP‐1‐, 5‐HT‐, and neurotensin‐expressing enteroendocrine cells in rats." Neurogastroenterology & Motility 25.1 (2013): e70-e79. Tested in Rat, PubMed ID: 23095091

Dalvi, Prasad S., et al. "Glucagon-like peptide-1 receptor agonist, exendin-4, regulates feeding-associated neuropeptides in hypothalamic neurons in vivo and in vitro." Endocrinology 153.5 (2012): 2208-2222.

Dalvi, Prasad S., and Denise D. Belsham. "Glucagon-like peptide-2 directly regulates hypothalamic neurons expressing neuropeptides linked to appetite control in vivo and in vitro." Endocrinology 153.5 (2012): 2385-2397. PubMed ID: 22416082

Cui, Huxing, et al. "Neuroanatomy of melanocortin‐4 receptor pathway in the lateral hypothalamic area." Journal of Comparative Neurology 520.18 (2012): 4168-4183. Tested in Mouse, PubMed ID: 22605619

Kortleven, Christian, Laura Charlotte Bruneau, and Louis-Eric Trudeau. "Neurotensin inhibits glutamate-mediated synaptic inputs onto ventral tegmental area dopamine neurons through the release of the endocannabinoid 2-AG." Neuropharmacology 63.6 (2012): 983-991.

Zahm, Daniel S., et al. "Inputs to the midbrain dopaminergic complex in the rat, with emphasis on extended amygdala‐recipient sectors." Journal of Comparative Neurology 519.16 (2011): 3159-3188. Tested in Rat, PubMed ID: 21618227

Leinninger, Gina M., et al. "Leptin action via neurotensin neurons controls orexin, the mesolimbic dopamine system and energy balance." Cell metabolism 14.3 (2011): 313-323. Tested in Mouse, PubMed ID: 21907138

Daniel D. Lama, Ligang Zhoub, Andreas Veggea,c, Philip Y. Xiua, Britt T. Christensena,C, Mayowa A. Osundijid, Chen-yu Yuehd,E, Mark L. Evansd, and Lora K. Heislera; Distribution and neurochemical characterization of neurons within the nucleus of the solitary tract responsive to serotonin agonist-induced hypophagia; Behav Brain Res,196:139-143 (2009), Tested in Rat, PubMed ID: 18762217

Geerling, Joel Charles. Aldosterone-sensitive Neurons and Salt Appetite. ProQuest, 2008. Tested in Rat.

Geerling, Joel C., Mitsuhiro Kawata, and Arthur D. Loewy. "Aldosterone‐sensitive neurons in the rat central nervous system." Journal of Comparative Neurology 494.3 (2006): 515-527. Tested in Rat, PubMed ID: 16320254

Sequeira, S. M., J. C. Geerling, and A. D. Loewy. "Local inputs to aldosterone-sensitive neurons of the nucleus tractus solitarius." Neuroscience 141.4 (2006): 1995-2005. Tested in Rat, PubMed ID: 16828976

Geisler, Stefanie, and Daniel S. Zahm. "On the retention of neurotensin in the ventral tegmental area (VTA) despite destruction of the main neurotensinergic afferents of the VTA—implications for the organization of forebrain projections to the VTA." Brain research 1087.1 (2006): 87-104. Tested in Rat, PubMed ID: 16626637

Cui, Hong, Fang Cai, and Denise D. Belsham. "Anorexigenic hormones leptin, insulin, and α-melanocyte-stimulating hormone directly induce neurotensin (NT) gene expression in novel NT-expressing cell models." The Journal of neuroscience 25.41 (2005): 9497-9506. Tested in Mouse, PubMed ID: 16221860

Krout, Karl E., et al. "CNS neurons with links to both mood-related cortex and sympathetic nervous system." Brain research 1050.1 (2005): 199-202. PubMed ID: 15975562

Shroyer, Wallis,Venken ,Bellen, Zoghbi; Gfi1 functions downstream of Math1 to control intestinal secretory cell subtype allocation and differentiation; Genes & Development,19:2412-2417 (2005), Tested in Mouse, PubMed ID: 16230531

Fudge, J. L. "Bcl-2 immunoreactive neurons are differentially distributed in subregions of the amygdala and hippocampus of the adult macaque." Neuroscience 127.2 (2004): 539-556. Tested in Monkey, PubMed ID: 15262342

Kultas‐Ilinsky, Kristy, Catherine Fallet, and Catherine Verney. "Development of the human motor‐related thalamic nuclei during the first half of gestation, with special emphasis on GABAergic circuits." Journal of Comparative Neurology 476.3 (2004): 267-289. Tested in Human, PubMed ID: 15269970

Martorana, A., et al. "Enkephalin, neurotensin, and substance P immunoreactivite neurones of the rat GP following 6-hydroxydopamine lesion of the substantia nigra." Experimental neurology 183.2 (2003): 311-319. Tested in Rat, PubMed ID: 14552872

Villeneuve, Pierre, et al. "Altered processing of the neurotensin/neuromedin N precursor in PC2 knock down mice: a biochemical and immunohistochemical study." Journal of neurochemistry 82.4 (2002): 783-793. Tested in Mouse, PubMed ID: 12358783

Jimenez, B., et al. "Occurrence, co‐occurrence and topographic distribution of choline acetyl transferase, Met‐enkephalin and neurotensin in the stellate ganglion of the cat." Synapse 43.3 (2002): 163-174. Tested in Cat, PubMed ID: 11793421

Moore, Robert Y., Joan C. Speh, and Rehana K. Leak. "Suprachiasmatic nucleus organization." Cell and tissue research 309.1 (2002): 89-98. Tested in Rat, PubMed ID: 12111539

Zahm, D. S., et al. "Neurons of origin of the neurotensinergic plexus enmeshing the ventral tegmental area in rat: retrograde labeling andin situ hybridization combined." Neuroscience 104.3 (2001): 841-851. Tested in Rat, PubMed ID: 11440814

Yang, Qi, et al. "Requirement of Math1 for secretory cell lineage commitment in the mouse intestine." Science 294.5549 (2001): 2155-2158. Tested in Mouse, PubMed ID: 11739954

Gonzalez-Maciel, A., R. Reynosorobles, and M. Morales. "Ultrastructural Co-Localization of Methionine Enkephalin and Neurotensin in the Synapse of the Cat Stellate Ganglia." PROCEEDINGS-WESTERN PHARMACOLOGY SOCIETY. Vol. 44. [Western Pharmacology Society]; 1998, 2001. Tested in Cat, PubMed ID: 11793957

Villeneuve, Pierre, Nabil G. Seidah, and Alain Beaudet. "Immunohistochemical evidence for the implication of PCI in the processing of proneurotensin in rat brain." Neuroreport 11.16 (2000): 3443-3447. Tested in Rat, PubMed ID: 11095496

Villeneuve, Pierre, et al. "Immunohistochemical evidence for the involvement of protein convertases 5A and 2 in the processing of pro‐neurotensin in rat brain." Journal of Comparative Neurology 424.3 (2000): 461-475. Tested in Rat, PubMed ID: 10906713

Javed, A., et al. "D-Fenfluramine induces serotonin-mediated Fos expression in corticotropin-releasing factor and oxytocin neurons of the hypothalamus, and serotonin-independent Fos expression in enkephalin and neurotensin neurons of the amygdala." Neuroscience 90.3 (1999): 851-858. PubMed ID: 10218785

Miller, K. E., and A. T. Salvatierra. "Apposition of enkephalin-and neurotensin-immunoreactive neurons by serotonin-immunoreactive varicosities in the rat spinal cord." Neuroscience 85.3 (1998): 837-846. Tested in Rat, PubMed ID: 9639277

Zahm, Daniel S., Evelyn S. Williams, and James E. Krause. "Desensitization and enhancement of neurotensin/neuromedin N mRNA responses in subsets of rat caudate-putamen neurons following multiple administrations of haloperidol." Molecular brain research 59.2 (1998): 196-204. Tested in Rat, PubMed ID: 9729381

Duan, Xiaoqin, and Gong Ju. "The organization of chemically characterized afferents to the perivascular neuronal groups of the hypothalamic magnocellular neurosecretory system in the rat." Brain research bulletin 46.5 (1998): 409-415. Tested in Rat, PubMed ID: 9739002

Nakamura, Hiroyuki, et al. "Involvement of central neurotensin in thermoregulatory and neuroimmune function in pregnant rats exposed to heat." Brain, behavior, and immunity 11.2 (1997): 141-152. Tested in Rat, PubMed ID: 9299063

Seelentag, Walter KF, et al. "CD44 isoform expression in the diffuse neuroendocrine system. I. Normal cells and hyperplasia." Histochemistry and cell biology 106.6 (1996): 543-550. PubMed ID: 8985742

Brog, J. S., and D. S. Zahm. "Morphologically distinct subpopulations of neurotensin-immunoreactive striatal neurons observed in rat following dopamine depletions and D 2 receptor blockade project to the globus pallidus." Neuroscience 74.3 (1996): 805-812. Tested in Rat, PubMed ID: 8884776

Upchurch, Brent H., et al. "Peptide YY expression is an early event in colonic endocrine cell differentiation: evidence from normal and transgenic mice." Development 122.4 (1996): 1157-1163. Tested in Mouse, PubMed ID: 8620842

Biankin, Andrew V. "Endocrine cells of transitional mucosa adjacent to colonic adenocarcinoma." Australian and New Zealand journal of surgery 65.5 (1995): 334-338. PubMed ID: 7741677

Brog, J. S., and D. S. Zahm. "Morphology and Fos immunoreactivity reveal two subpopulations of striatal neurotensin neurons following acute 6-hydroxydopamine lesions and reserpine administration." Neuroscience 65.1 (1995): 71-86. PubMed ID: 7753409

Seki, Tatsunori, Sakaé Kikuyama, and Noboru Yanaihara. "Morphology of the skin glands of the crab-eating frog (Rana cancrivora)." Zoological Science 12.5 (1995): 623-626. Tested in Frog.

Allen, G. V., R. T. F. Cheung, and D. F. Cechetto. "Neurochemical changes following occlusion of the middle cerebral artery in rats." Neuroscience 68.4 (1995): 1037-1050. Tested in Rat, PubMed ID: 8544980

Allen, G. V., and D. F. Cechetto. "Neurotensin in the lateral hypothalamic area: origin and function." Neuroscience 69.2 (1995): 533-544.     PubMed ID: 8552247

Lopez, M. James, et al. "Studies in transgenic mice reveal potential relationships between secretin-producing cells and other endocrine cell types." Journal of Biological Chemistry 270.2 (1995): 885-891. Tested in Mouse and Rat, PubMed ID: 7822327

Cheung, R. T. F., T. Diab, and D. F. Cechetto. "Time‐course of neuropeptide changes in peri‐ischemic zone and amygdala following focal ischemia in rats." Journal of Comparative Neurology 360.1 (1995): 101-120. Tested in Rat, PubMed ID: 7499557

Berk, Mitchell L., and Stacy E. Smith. "Local and commissural neuropeptide‐containing projections of the nucleus of the solitary tract to the dorsal vagal complex in the pigeon." Journal of Comparative Neurology 347.3 (1994): 369-396. Tested in Pigeon, PubMed ID: 7529775

Berger, Brigitte, and Chantal Alvarez. "Neurochemical development of the hippocampal region in the fetal rhesus monkey. II. Immunocytochemistry of peptides, calcium‐binding proteins, DARPP‐32, and monoamine innervation in the entorhinal cortex by the end of gestation." Hippocampus 4.1 (1994): 85-114. Tested in Monkey, PubMed ID: 7914799

Woulfe, J., et al. "Post-translational processing of the neurotensin/neuromedin N precursor in the central nervous system of the rat—II. Immunohistochemical localization of maturation products." Neuroscience 60.1 (1994): 167-181. Tested in Rat, PubMed ID: 8052410

Senger, B., J. S. Brog, and D. S. Zahm. "Subsets of neurotensin-immunoreactive neurons in the rat striatal complex following antagonism of the dopamine D 2 receptor: An immunohistochemical double-labeling study using antibodies against Fos." Neuroscience 57.3 (1993): 649-660. Tested in Rat, PubMed ID: 8309528

Rinaman, Linda, et al. "Distribution and neurochemical phenotypes of caudal medullary neurons activated to express cFos following peripheral administration of cholecystokinin." Journal of Comparative Neurology 338.4 (1993): 475-490. PubMed ID: 8132858

Sim, Laura J., and Shirley A. Joseph. "Dorsal raphe nucleus efferents: termination in peptidergic fields." Peptides 14.1 (1993): 75-83. PubMed ID: 8441710

Yaqub, A., and W. D. Eldred. "Effects of excitatory amino acids on immunocytochemically identified populations of neurons in turtle retina." Journal of neurocytology 22.8 (1993): 644-662. Tested in Turtle, PubMed ID: 8229090

Eissele, R., et al. "Immunoreactivity and expression of amylin in gastroenteropancreatic endocrine tumors." The American journal of pathology 143.1 (1993): 283. Tested in Human, PubMed ID: 8317551

Tsalis, K. G., et al. "Intracerebroventricular infusion of neurotensin suppresses gastric acid secretion in dogs." European surgical research 25.1 (1993): 52-59. Tested in Dog, PubMed ID: 8482306

Zhang, R. X., et al. "Morphological evidence for the activation of descending modulatory control by nociceptive afferent pathways: an immunocytochemical study." Brain research 603.1 (1993): 162-165. PubMed ID: 7680938

Berger, Brigitte, Chantal Alvarez, and Patricia S. Goldman‐Rakic. "Neurochemical development of the hippocampal region in the fetal rhesus monkey. I. Early appearance of peptides, calcium‐binding proteins, DARPP‐32, and monoamine innervation in the entorhinal cortex during the first half of gestation (E47 to E90)." Hippocampus 3.3 (1993): 279-305. Tested in Monkey, PubMed ID: 8353610

Berk, Mitchell L., Stacy E. Smith, and Harvey J. Karten. "Nucleus of the solitary tract and dorsal motor nucleus of the vagus nerve of the pigeon: Localization of peptide and 5‐hydroxytryptamine immunoreactive fibers." Journal of Comparative Neurology 338.4 (1993): 521-548. Tested in Pigeon, PubMed ID: 8132859

Marcos Martinez , Angel Hernanz , Cristina Grande  and Luis Felipe Pallardo; Plasma molecular forms of gastrin, neurotensin and somatostatin in pregnancy and gestational diabetes after an oral glucose load or a mixed meal; Regulatory Peptides,,47:73-80 (1993), Tested in Human, PubMed ID: 8105515

Morales, M. A., et al. "Ultrastructural localization of neurotensin immunoreactivity in the stellate ganglion of the cat." Journal of neurocytology 22.12 (1993): 1017-1021. Tested in Cat, PubMed ID: 8106876

Adams, J. C. "Biotin amplification of biotin and horseradish peroxidase signals in histochemical stains." Journal of Histochemistry & Cytochemistry 40.10 (1992): 1457-1463. PubMed ID: 1527370

Woulfe, John, and Alain Beaudet. "Neurotensin terminals form synapses primarily with neurons lacking detectable tyrosine hydroxylase immunoreactivity in the rat substantia nigra and ventral tegmental area." Journal of Comparative Neurology 321.1 (1992): 163-176. Tested in Rat, PubMed ID: 1351897

Zahm, D. S. "Subsets of neurotensin-immunoreactive neurons revealed following antagonism of the dopamine-mediated suppression of neurotensin immunoreactivity in the rat striatum." Neuroscience 46.2 (1992): 335-350. Tested in Rat, PubMed ID: 1542410

Rubin, Deborah C., et al. "Use of fetal intestinal isografts from normal and transgenic mice to study the programming of positional information along the duodenal-to-colonic axis." Journal of Biological Chemistry 267.21 (1992): 15122-15133. Tested in Mouse, PubMed ID: 1634547

Tsalis, K. G., et al. "Changes in gastric secretion after intracerebroventricular infusion of bombesin in dogs." Scandinavian journal of gastroenterology 25.6 (1990): 563-571. Tested in Dog, PubMed ID: 2359987

Roth, Kevin A., Jennifer M. Hertz, and Jeffrey I. Gordon. "Mapping enteroendocrine cell populations in transgenic mice reveals an unexpected degree of complexity in cellular differentiation within the gastrointestinal tract." The Journal of cell biology 110.5 (1990): 1791-1801. Tested in Mouse, PubMed ID: 2186049

Sithigorngul, Paisarn, Antony OW Stretton, and Cynthia Cowden. "Neuropeptide diversity in Ascaris: an immunocytochemical study." Journal of Comparative Neurology 294.3 (1990): 362-376. PubMed ID: 2341616

Seybold, Virginia S., et al. "Neurotensin binding sites in porcine jejunum: biochemical characterization and intramural localization." Synapse 6.1 (1990): 81-90. PubMed ID: 2169072

Zahm, D. S., et al. "Postnatal development of striatal neurotensin immunoreactivity in relation to clusters of substance P immunoreactive neurons and the “dopamine islands” in the rat." Journal of Comparative Neurology 296.3 (1990): 403-414. Tested in Rat, PubMed ID: 1694190

McCarson, Kenneth E., and Barry D. Goldstein. "Time course of the alteration in dorsal horn substance P levels following formalin: blockade by naloxone." Pain 41.1 (1990): 95-100. PubMed ID: 1693764

Tuchscherer, M. M., and V. S. Seybold. "A quantitative study of the coexistence of peptides in varicosities within the superficial laminae of the dorsal horn of the rat spinal cord." The Journal of neuroscience 9.1 (1989): 195-205. Tested in Rat, PubMed ID: 2643683

Zahm, D. S., and S. N. Johnson. "Asymmetrical distribution of neurotensin immunoreactivity following unilateral injection of 6-hydroxydopamine in rat ventral tegmental area (VTA)." Brain research 483.2 (1989): 301-311. Tested in Rat, PubMed ID: 2495843

Tay, Samuel SW, Terence H. Williams, and Jean Y. Jew. "Neurotensin immunoreactivity in the central nucleus of the rat amygdala: an ultrastructural approach." Peptides 10.1 (1989): 113-120. Tested in Rat, PubMed ID: 2748415

Zahm, D. S. "The ventral striatopallidal parts of the basal ganglia in the rat—II. Compartmentation of ventral pallidal efferents." Neuroscience 30.1 (1989): 33-50. Tested in Rat, PubMed ID: 2473414

Hu, Haitao, et al. "Distribution pattern of cell bodies and fibers with neurotensinlike immunoreactivity in the cat hypothalamus." Journal of Comparative Neurology 272.2 (1988): 269-279. Tested in Cat, PubMed ID: 3397408

Sugimoto, Tetsuo, et al. "Changes of neurotensinlike immunoreactivity in the striatum of the cat after intrastriatal injection of kainic acid." Journal of Comparative Neurology 263.4 (1987): 607-612. Tested in Cat, PubMed ID: 3667991

Shipley, Michael T., John H. McLean, and Michael M. Behbehani. "Heterogeneous distribution of neurotensin-like immunoreactive neurons and fibers in the midbrain periaqueductal gray of the rat." The Journal of neuroscience 7.7 (1987): 2025-2034. Tested in Rat, PubMed ID: 3302124

Paré, Michel, Laurent Descarries, and Leif Wiklund. "Innervation and reinnervation of rat inferior olive by neurons containing serotonin and substance P: an immunohistochemical study after 5, 6-dihydroxytryptamine lesioning." Journal of neurocytology 16.2 (1987): 155-167. PubMed ID: 2442314

Block, Christine H., and Gloria E. Hoffman. "Neuropeptide and monoamine components of the parabrachial pontine complex." Peptides 8.2 (1987): 267-283. PubMed ID: 2884646

Zahm, Daniel S. "Neurotensin-immunoreactive neurons in the ventral striatum of the adult rat: ventromedial caudate-putamen, nucleus accumbens and olfactory tubercle." Neuroscience letters 81.1 (1987): 41-47. Tested in Rat, PubMed ID: 3696473

Krukoff, Teresa L. "NPY-containing nerve terminals and fibers in close association with peptidergic sympathetic preganglionic neurons." Neuroscience letters 77.1 (1987): 1-4. PubMed ID: 2439953

Weihe, E. "Peripheral innervation of the heart." Silent Ischemia. Steinkopff, 1987. 7-18.

Sugimoto, Tetsuo, and Noboru Mizumo. "Immunohistochemical demonstration of neurotensin in striatal neurons of the cat, with particular reference to coexistence with enkephalin." Brain research 398.1 (1986): 195-198. Tested in Cat, PubMed ID: 3542122

Cassell, M. D., T. S. Gray, and J. Z. Kiss. "Neuronal architecture in the rat central nucleus of the amygdala: a cytological, hodological, and immunocytochemical study." Journal of Comparative Neurology 246.4 (1986): 478-499. Tested in Rat, PubMed ID: 2422231

Müller-Hermelink, H. K., M. Marino, and G. Palestro. "Pathology of thymic epithelial tumors." The Human Thymus. Springer Berlin Heidelberg, 1986. 207-268.

Wolfson, B., et al. "A quantitative, immunochemical demonstration of the postnatal development of neurotensin in the medial preoptic area of the rat." Developmental Brain Research 18.1 (1985): 241-250. Tested in Rat, PubMed ID: 3886084

Sugimoto, Tetsuo, et al. "Coexistence of neuropeptides in projection neurons of the thalamus in the cat." Brain research 347.2 (1985): 381-384. Tested in Cat, PubMed ID: 3904921

Newton, Bruce W., and Bruce E. Maley. "Distribution of neurotensin-like immunoreactivity in rat and cat area postrema." Peptides 6.2 (1985): 301-306. Tested in Rat and Cat, PubMed ID: 3898039

Berrettini, Wade H., et al. "Neuropeptides in human cerebrospinal fluid." Life sciences 37.13 (1985): 1265-1270. Tested in Human.

Moody, Terry W., et al. "Neurotensin is produced by and secreted from classic small cell lung cancer cells." Life sciences 36.18 (1985): 1727-1732. PubMed ID: 2984506

Hoffman, Gloria E. "Organization of LHRH cells: differential apposition of neurotensin, substance P and catecholamine axons." Peptides 6.3 (1985): 439-461. PubMed ID: 2415951

Baldino Jr, F., et al. "Primary explants as a model of the hypothalamusin situ." Peptides 6.2 (1985): 249-256.

Schmidt, D., D. Harms, and W. Lang. "Primary malignant hepatic tumours in childhood." Virchows Archiv A 407.4 (1985): 387-405. PubMed ID: 2413614

Davis, Leonard G., et al. "The application of enzyme-linked immunosorbent assays (ELISA) to neuropeptides." Journal of neuroscience methods 14.1 (1985): 15-23.

Köhler, Christer, and Lars G. Eriksson. "An immunohistochemical study of somatostatin and neurotensin positive neurons in the septal nuclei of the rat brain." Anatomy and embryology 170.1 (1984): 1-10. Tested in Rat, PubMed ID: 6148028

Mantyh, Patrick W., and Stephan P. Hunt. "Neuropeptides are present in projection neurones at all levels in visceral and taste pathways: from periphery to sensory cortex." Brain research 299.2 (1984): 297-312. PubMed ID: 6733452

Seybold, Virginia S., and Bruce Maley. "Ultrastructural study of neurotensin immunoreactivity in the superficial laminae of the dorsal horn of the rat." Peptides 5.6 (1984): 1179-1189. Tested in Rat, PubMed ID: 6531271

Beitz, Alvin J., R. David Shepard, and William E. Wells. "The periaqueductal gray-raphe magnus projection contains somatostatin, neurotensin and serotonin but not cholecystokinin." Brain research 261.1 (1983): 132-137. PubMed ID: 6132659

Maley, B., and R. Elde. "Immunohistochemical localization of putative neurotransmitters within the feline nucleus tractus solitarii." Neuroscience 7.10 (1982): 2469-2490. Tested in Cat, PubMed ID: 6184644

Emson PC, Goedert M, Williams B, Ninkovic M, Hunt SP.; Neurotensin - Regional Distribution, Characterization, and Inactivation; Ann N Y Acad Sci.,400:198-215 (1982), Tested in Rat, PubMed ID: 6762831

Seybold, Virginia S., and Robert P. Elde. "Neurotensin immunoreactivity in the superficial laminae of the dorsal horn of the rat: I. Light microscopic studies of cell bodies and proximal dendrites." Journal of Comparative Neurology 205.1 (1982): 89-100. Tested in Rat, PubMed ID: 7040502

Bodnar, Richard J., et al. "THE EFFECTS OF CENTRALLY ADMINISTERED ANTISERA TO NEUROTENSIN AND RELATED PEPTIDES UPON NOCICEPTION AND RELATED BEHAVIORS*." Annals of the New York Academy of Sciences 400.1 (1982): 244-258. PubMed ID: 6188400

Beitz, ALVIN J. "The sites of origin brain stem neurotensin and serotonin projections to the rodent nucleus raphe magnus." The Journal of Neuroscience 2.7 (1982): 829-842. Tested in Rat, PubMed ID: 6178807

Leranth, C. S., et al. "Stria terminalis axons ending on substance P and neurotensin-containing cells of rat central amygdaloid nucleus: an electron microscopic immunocytochemical study." Neuropeptides 1.4 (1981): 261-272. Tested in Rat.

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Product Reviews

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Reviewed by droot
07/10/2012 - 10:12:41 PM
Visualization of ventromedial ventral pallidum
For our manuscript, Differential roles of ventral pallidum subregions during cocaine self-administration behaviors, in press at Journal of Comparative Neurology, we used the Immunostar rabbit anti-neurotensin primary antibody to visualize the ventromedial ventral pallidum subregion.

Rats were perfused with saline followed by 4% PF, stored in 30% sucrose, and coronally sectioned to 40 um. We followed the protocols of Zahm/Heimer and colleagues when they discovered the ventral pallidum subregions and their afferent/efferent projection patterns.

The protocol consisted of the following steps:
1. Wash in 0.1M phosphate buffer (PB; pH 7.4)
2. 15 min in 1% sodium borohydride
3. Wash
4. 1 hour blocking with PB containing 0.1% Triton X-100 and 3% normal goat serum
5. Primary antibody overnight at 4C - ImmunoStar rabbit anti-neurotensin diluted 1 : 6500 in PB containing 0.1% Triton X-100 and 3% normal goal serum
6. Wash
7. 1 hour anti-rabbit biotinylated secondary (Vector) 1:200 in PB with 0.1% Triton X-100
8. Wash
9. 1 hour ABC (Vector) 1:200 in PB with 0.1% Triton X-100
10. Wash
11. Develop with 0.05% DAB for 6 min

Our study involved recording neurons within the ventral pallidum subregions during specific aspects of intravenous cocaine self-administration (approaching toward, responding on, or retreating away from a cocaine-reinforced operandum). In order to verify the placement of microwires within the VP subregions, prior to perfusion we passed current through each stainless steel microwire to leave an iron deposit at the uninsulated tip. After DAB (brown reaction) and mounting, we visualized the iron deposit by incubating in a 5% potassium ferrocyanide and 10% HCl solution (leaving a blue-green reaction).

For our purposes, this antibody labeled fibers in ventromedial VP with low background. Individual neurons and other fibers were clearly observed within BNST that were not involved in our study.

I rated this antibody 4 stars because there was some variability for this stain between animals.

David H. Root, Ph.D.
Rutgers University
Reviewed by Dan_Tylee
04/05/2012 - 01:09:25 PM
Crisp, Cell-specific labeling
Tissue: Maccaca fasciscularis, perfused with saline and 4% paraformaldehyde, dehydrated through increase sucrose gradients, sectioned at 40 um with sliding microtome.

Method: Immunocytochemistry on free floating sections. Incubated with ImmunoStar's primary antibody at a concentration of 1:1000 for 4 days in 10% normal goat serum (in 0.1M PO4 buffer with .3% Triton X-100). Rinsed, then incubated with Vector Lab's biotinylated goat anti-rabbit secondary antibody (#BA-1000) at a concentration of 1:200 for 40 minutes at room temperature in the same 10% normal goat serum solution. Rinsed, then incubated with Vector's standard peroxidase kit (#PK-4000) for 60 minutes at room temperature in 0.1M PO4 buffer with .3% Triton X-100. Rinsed, then developed using the instructions for the kit.

Results: Crisp, cell-specific labeling. Low background. Very helpful for determining the boundary between the medial and lateral core divisions of the central nucleus of the amygdala.

Daniel Tylee
Department of Neurobiology & Anatomy
University of Rochester - School of Medicine and Dentistry