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Neuropeptide Y Antibody

Neuropeptide Y Antibody
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  • Image is NPY interneurons (RED) in mouse striatum, GREEN staining is GFP expressed by medium spiny neurons.
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Price: $275.00
Product ID : 22940
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Description

Neuropeptide Y (NPY) is a member of a regulatory peptide family and has a marked sequence homology with pancreatic polypeptide (PP) and peptide YY (PYY), which are other members of the family.

The second image above entitled "C." is NPY interneurons (RED) in mouse striatum, GREEN staining is GFP expressed by medium spiny neurons provided Alipi Naydenov, Neurobiology and Behavior, Stella Lab University of Washington (see review below).

In the rat central nervous system, immunohistochemistry has found NPY-like cell bodies in the cortex, caudate-putamen, hypothalamus (arcuate nucleus), hippocampus, anterior westolfactory bulb, nucleus accumbens, amygdaloid complex and periaqueductal grey. NPY-like fibers and terminals are detected in high numbers in the bed nucleus of the stria terminalis, the peri- and paraventricular regions of the hypothalamus and thalamus and in discrete hypothalamic nuclei, particularly the suprachiasmatic nucleus.

Westerns are not recommended for the Neuropeptide Y antibody since the protein is too small to detect in gel.


Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Fish, Frog, Guinea Pig, Hamster, Human, Mouse, Rat, Squirrel, Zebrafish

Availability: In Stock

Preabsorption Control: Available at Sigma Aldrich, Catalog # N3266

Alternate Names:  Pro-neuropeptide Y; Neuropeptide tyrosine; prepro-neuropeptide Y; PYY4

Gene Symbol / ID, Accession #:  NPY, 4852


Download Product Spec. SheetNeuropeptide Y Antibody


Journal References:


Disruption of the Gene Encoding SF-1 Alters the Distribution of Hypothalamic Neuronal Phenotypes; Tammy L. Dellovade, Morag Young,Elizabeth P. Ross,Rachael Henderson, Kathleen Caron,Keith Parker,Stuart A. Tobet; Journal of comparative neurology, 423(4):579-89, 2000, Tested in Mouse, Cites Diasorin AKA ImmunoStar, PubMed ID: 10880989

Special Relationship of gamma-Aminobutyric Acid to the Ventromedial Nucleus of the Hypothalamus During Embryonic Development; Stuart A. Tobet,  Rachel G. Henderson, Paul J. Whiting, Werner Sieghart; The Journal of Neuroscience,405(1):88-98, 1999, Tested in Mouse, Cites Diasorin AKA ImmunoStar, PubMed ID: 10022198

Histogenetic compartments of the mouse centromedial and extended amygdala based on gene expression patterns during development.; García-López M, Abellán A, Legaz I, Rubenstein JL, Puelles L, Medina L; J Comp Neurol.,506(1):46-74, 2008, Tested in Mouse, Cites Diasorin AKA ImmunoStar, PubMed ID: 17990271

Insulin suppresses ghrelin-induced calcium signaling in neuropeptide Y neurons of the hypothalamic arcuate nucleus; Maejima Y, Kohno D, Iwasaki Y, Yada T; AGING,3(11):1092–1097, 2011, Tested in Rat, IFC, Cites Diasorin AKA ImmunoStar, PubMed ID: 22081645

Pre-Emptive Treatment of Lidocaine Attenuates Neuropathic Pain and Reduces Pain-Related Biochemical Markers in the Rat Cuneate Nucleus in Median Nerve Chronic Constriction Injury Model; Chi-Te Lin, Yi-Ju Tsai, Hsin-Ying Wang, Seu-Hwa Chen, Tzu-Yu Lin, and June-Horng Lue; Anesthesiology Research and Practice, 2012:921405-14, 2012, Tested in Rat, IFC, Cites Diasorin AKA ImmunoStar, PubMed ID: 22162685

Immunocytochemical study on the liver innervation in patients with cirrhosis.; Stoyanova II, Gulubova MV; acta histochem,102:391-402, 2000, Tested in Human , Cites Incstar AKA ImmunoStar, PubMed ID: 11145532

Origin and Molecular Specification of Striatal Interneurons; Marin O, Anderson SA, Rubenstein JLR; The Journal of Neuroscience,20(16):6063-6076, 2000, Tested in Mouse, Cites Incstar AKA ImmunoStar, PubMed ID: 10934256

Age-related change in the neuropeptide Y and NADPH-diaphorase-positive neurons in the cerebral cortex and striatum of aged rats; Youngbuhm Huha, Changok Kimb, Wonkyu Leea, Junghye Kima, Heekyung Ahna; Neuroscience Letters, 223:157-160, 1997, Tested in Rat, Cites Incstar AKA ImmunoStar, PubMed ID: 9080456

Intrinsic Bias and Lineage Restriction in the Phenotype Determination of Dopamine and Neuropeptide Y Amacrine Cells; Moody SA, Chow  I, Huang S; The Journal of Neuroscience, 20(9):3244-3253, 2000, Tested in Frog, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 10777789

Spatiotemporal Patterns of Activity in an Intact Mammalian Network with Single-Cell Resolution: Optical Studies of Nicotinic Activity in an Enteric Plexus; Obaid AL, Koyano T, Lindstom J; The Journal of Neuroscience, 19(8):3073-3093, 1999, Tested in Guinea Pig, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 10191324

The origin of neocortical nitric oxide synthase-expressing inhibitory neurons; Xavier H.Jaglin, JensHjerling-Leffler, GordFishell, and RenataBatista-Brito; Frontiers in Neural Circuits,6:44, 2012, Tested in Mouse, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 22787442

The mouse olfactory peduncle; Brunjes PC, Kay RB, Arrivillaga JP.; J Comp Neurol.,518(14):2870-86, 2011, Tested in Mouse, PubMed ID: 21618219

Distinct cis-Regulatory Elements from the Dlx1,Dlx2 Locus; Noe¨l Ghanem, Man Yu, Jason Long, Gary Hatch, John L. R. Rubenstein, and Marc Ekker; The Journal of Neuroscience, 27(19):5012–5022, 2007, Tested in Mouse, PubMed ID: 17494687

Food deprivation-induced changes in body fat mobilization after neonatal monosodium glutamatetreatment; Claudia Leitner and Timothy J. Bartness; American Journal of Physiology, 294:R775-R783, 2008, Tested in Hamster, PubMed ID: 18094070

Acute brown adipose tissue temperature response to cold in monosodium glutamate-treated Siberian hamsters; Claudia Leitner, Timothy J. Bartness; Brain Research, 1292:3 8–5, 2009, Tested in Hamster, PubMed ID: 19643091

Neonatal loss of γ–aminobutyric acid pathway expression after human perinatal brain injury; Shenandoah Robinson, M.D., Qing Li, M.D., Anne DeChant, M.S., and Mark L. Cohen, M.D.; J Neurosurg, 104:396-408, 2006, Tested in Human , PubMed ID: 16776375

Effect of centrally administered C75, a fatty acid synthase inhibitor, on ghrelin secretion and its downstream effects; Hu, Cha, Haasteren, Wang, Lane; Proceedings of  the National Academy of Sci., 102(11):3972-3977, 2005, Tested in Mouse, PubMed ID: 15728730

Collagen XIX is expressed by interneurons and contributes to the formation of hippocampal synapses; Su J, Gorse K, Ramirez F, Fox MA.; J Comp Neurol., 518(2):229-53, 2010, Tested in Mouse, PubMed ID: 19937713

Roles for c-Aminobutyric Acid in the Development of the Paraventricular Nucleus of the Hypothalamus; Kristy M. McClellan, Matthew S. Stratton, and Stuart A. Tobet; J Comp Neurol. 2010; 518(14):2710-29, 2010, Tested in Mouse, PubMed ID: 20506472

The decreased cellular expression of neuropeptide Y protein in rat brain structures during ethanol withdrawal after chronic ethanol exposure; Roy A, Pandey SC; Alcoholism: clinical and experimental research, 26(6):796-803, 2002, Tested in Rat, PubMed ID: 12068247

Effects of PKA modulation on the expression of neuropeptide Y in rat amygdaloid structures during ethanol withdrawal; Huaibo Zhang, Subhash C. Pandey; Peptides, 24:1397-1402, 2003, Tested in Rat, PubMed ID: 14706555

Effects of acupuncture on chronic corticosterone-induced depression-like behavior and expression of neuropeptide Y in the rats; Bombi Leea, Insop Shimb, Hye-Jung Leea, Young Yangc, Dae-Hyun Hahma; Neuroscience Letters, 0304-3940, 2009, Tested in Rat, PubMed ID: 19429024

Simultaneous Silencing of Npy and Dbh Expression in Hindbrain A1/C1 Catecholamine Cells Suppresses Glucoprivic Feeding; Ai-Jun Li, Qing Wang, Thu T. Dinh, and Sue Ritter; Journal of Neuroscience, 29(1):280 –287, 2009, Tested in Rat, PubMed ID: 19129404

A Trigeminoreticular Pathway: Implications in Pain; W. Michael Panneton, Qi Gan, Robert S. Livergood; PLoS One,6:1-15, 2011, Tested in Rat, PubMed ID: 21957454

Changes in neuropeptide Y immunoreactivity and transcript levels in circadian system structures of the diurnal rodent, the thirteenlined ground squirrel; Luis Vidal and Nidza Lugo; Brain Res, 1125:77-84, 2006, Tested in Squirrel, PubMed ID: 17109825

Neuronal relationship between orexin-A- and neuropeptide Y-induced orexigenic actions in goldfish; Kenji Kojima, Motoki Kamijo, Haruaki Kageyama, Minoru Uchiyama, Seiji Shioda, Kouhei Matsuda ; Neuropeptides, 43:63–71, 2009, Tested in Fish, PubMed ID: 19261328

Changes in Rx1 and Pax6 activity; Norann A. Zaghloul, Sally A. Moody; Molecular Vision, 13:86-95, 2007, Tested in Frog, PubMed ID: 17277735

Contexts for dopamine specification by calcium spike activity in the central nervous system; Norma A. Velázquez-Ulloa, Nicholas C. Spitzer, Davide Dulcis; J Neurosci, 31(1):78-88, 2011, Tested in Frog, PubMed ID: 21209192

Monosodium glutamate-induced arcuate nucleus damage affects both natural torpor and 2DG-induced torpor-like hypothermia in Siberian hamsters; Kimberly M. Pelz, David Routman, Joseph R. Driscoll, Lance J. Kriegsfeld and John Dark; American Journal of Physiology, 294:R255-R265, 2008, Tested in Hamster, PubMed ID: 17959707

Origins of Cortical Interneuron Subtypes; Xu Qing, Cobos Inma, De La Cruz Estanislao , Rubenstein John L, Anderson Stewart A ; The Journal of NeuroScience, 24(11):2612-2622, 2004, Tested in Mouse, PubMed ID: 15028753

Gfi1 functions downstream of Math1 to control intestinal secretory cell subtype allocation and differentiation; Shroyer, Wallis,Venken ,Bellen, Zoghbi ; Genes & Development, 19:2412-2417, 2005, Tested in Mouse, PubMed ID: 16230531

Sonic hedgehog maintains the identity of cortical interneuron progenitors in the ventral telencephalon; Qing Xu, Carl P. Wonders, and Stewart A. Anderson; Development, 132 (22):4987-4998, 2005, Tested in Mouse, PubMed ID: 16221724

Cortical Inhibition Modified by Embryonic Neural Precursors Grafted into the Postnatal Brain ; Alvarez-Dolado, Calcagnotto, Karkar, Southwell, Jones-Davis, Estrada, Rubenstein, Alvarez-Buylla, and Baraban ; The Journal of Neuroscience, 26(28):7380-7389, 2006, Tested in Mouse, PubMed ID: 16837585

Spatial Genetic Patterning of the Embryonic Neuroepithelium Generates GABAergic Interneuron Diversity in the Adult Cortex; Matthew Fogarty, Matthew Grist, Diego Gelman, Oscar Marın, Vassilis Pachnis, and Nicoletta Kessaris; The Journal of Neuroscienc, 27(41):10935–10946, 2007, Tested in Mouse, PubMed ID: 17928435

A spatial bias for the origins of interneuron subgroups within the medial ganglionic eminence; Carl P. Wonder, Jelle Welagen, Lauren Taylor, Ihunanya C. Mbata, Jenny Z. Xiang, and Stewart A. Anderson; Dev Biol., 314:127-136, 2008, Tested in Mouse, PubMed ID: 18155689

Fate mapping Nkx2.1-lineage cells in the mouse telencephalon; Xu Q, Tam M, Anderson SA.; J Comp Neurol.,506(1):16-29, 2008, Tested in Mouse, PubMed ID: 17990269

Hypothalamic neurodegeneration and adult-onset obesity in mice lacking the Ubb polyubiquitin gene; Kwon-Yul Ryu, Jacob C. Garza, Xin-Yun Lu, Gregory S. Barsh, and Ron R. Kopito; PNAS, 105:4016-4021, 2008, Tested in Mouse, PubMed ID: 18299572

Molecular and Electrophysiological Characterization of GFP-Expressing CA1 Interneurons in GAD65-GFP Mice; Corette J. Wierenga, Fiona E. Mullner, Ilka Rinke, Tara Keck, Valentin Stein, Tobias Bonhoeffer; PLoS ONE, 5:1-11, 2010, Tested in Mouse, PubMed ID: 21209836

A novel functionally distinct subtype of striatal neuropeptide Y interneuron; Osvaldo Ibáñez-Sandoval, Fatuel Tecuapetla, Bengi Unal, Fulva Shah, Tibor Koós, James M. Tepper; J Neurosci., 31:16757-69, 2011, Tested in Mouse, PubMed ID: 22090502

Quantifying Biomarkers of Cognitive Dysfunction and Neuronal Network Hyperexcitability in Mouse Models of Alzheimer’s Disease: Depletion of Calcium-Dependent Proteins and Inhibitory Hippocampal Remodeling; Jorge J. Palop, Lennart Mucke, and Erik D. Roberson; Methods of Biol, 670:245-262, 2011, Tested in Mouse, PubMed ID: 20967595

Corticospinal Motor Neurons and Related Subcerebral; P. Ozdinler, Susanna Benn, Ted Yamamoto, Mine Guzel, Robert Brown, Jeff Ma ; The Journal of Neuroscience, 31(11):4166–4177, 2011, Tested in Mouse, PubMed ID: 21411657

Reelin is required for class-specific retinogeniculate targeting; Su J, Haner CV, Imbery TE, Brooks JM, Morhardt DR, Gorse K, Guido W, Fox MA.; J Neurosci, 31(2):575-86, 2011, Tested in Mouse, PubMed ID: 21228166

Functional Inactivation of the Genome-Wide Association Study Obesity Gene Neuronal Growth Regulator 1 in Mice Causes a Body Mass Phenotype; Angela W. S. Lee, Heidi Hengstler., Kathrin Schwald., Mauricio Berriel-Diaz, Desiree Loreth, Matthias Kirsch, Oliver Kretz, Carola A. Haas, Martin Hrabe de Angelis, Stephan Herzig, Thomas Bru¨mmendorf, Martin Klingenspor, Fritz G. Rathjen, Jan Rozman, George Nicholson, Roger D. Cox, Michael K. E. Schafer; PLoS ONE, 7(7):e41537, 2012, Tested in Mouse, PubMed ID: 22844493

Peripheral ghrelin transmits orexigenic signals through the noradrenergic pathway from the hindbrain to the hypothalamus; Yukari Date, Takuya Shimbara, Shuichi Koda, Koji Toshinai, Takanori Ida, Noboru Murakami, Mikiya Miyazato, Koichi Kokame, Yuta Ishizuka, Yasushi Ishida, Haruaki Kageyama, Seiji Shioda, Kenji Kangawa, and Masamitsu Nakazato; Cellular Neuroscience, 4(4):323-331, 2006, Tested in Rat, PubMed ID: 17011505

Structure and Autonomic Innervation of the Swim Bladder in the Zebrafish (Danio rerio); Finnely J, Robertson G, McGee C, Smith F, Croll R; The Journal of Comparative Neurology, 495:587-606, 2006, Tested in Zebrafish, PubMed ID: 16498679

Development of the Swimbladder and Its Innervation; G.N. Robertson, C.A.S. McGee, T.C. Dumbarton, R.P. Croll, and F.M. Smith; Journal of Morphology,268:967-985, 2007, Tested in Zebrafish, PubMed ID: 17702001

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Product Reviews

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Reviewed by user
12/18/2012 - 01:13:59 PM
Love the results
We have used your NPY(Product ID: 22940) antibody and love the results we obtain with this antibody. While it is not as clear as your Vasopressin antibody (Product ID: 20069), it works very well for our research purposes. For NPY we use a dilution of 1:3000 and amplify with tyramide solution at a 1:50 dilution. It is mixed with 10% serum, 10% biotin diluted in 80% PBS-T and incubated for 48-72 hours at 4 degrees C. We used brain tissue from Thamnophis sirtalis (Red-sided garter snake).

Submitted by:

Ashley Maine
Lutterschmidt Lab
Portland State University
Department of Biology
Portland, OR 97207
Reviewed by user
11/15/2012 - 11:47:31 AM
Antibody Performed Very Well
Above IFC image entitled "C." is NPY interneurons (RED) in mouse striatum, GREEN staining is GFP expressed by medium spiny neurons. NPY antibody was used at 1:1000 dilution with the staining protocol described below. Antibody performed very well. Scale bar = 25 microns.

Immunohistochemistry: Mice were euthanized and perfused with PFA (4% in PBS), post fixed for 24 hrs, and their brains cryoprotected in 15% sucrose (24 hrs) followed by 30% sucrose (48 hrs). Coronal sections that included the corticostriatal, globus pallidus, or substantia nigra regions (30 um) were prepared using a microtome and then stored in PBS at 4C until processing. Sections were processed/stained in parallel as follows: Free floating sections were rinsed 3x with PBS and incubated 90 min at room temperature (RT) in PBS supplemented with donkey serum (5%) and Triton X-100 (1%). Primary antibody combinations were prepared as a master stock in PBS supplemented with donkey serum (2.5%) and Triton X-100 (0.5%) and applied to sections for 72 hrs at 4°C with gentle agitation. Sections were then rinsed 8x with PBS supplemented with Tween-20 (0.05%, at RT) and incubated with a secondary antibodies diluted in PBS supplemented with donkey serum (2.5%) and Triton X-100 (0.5%) for 1 hr at RT with gentle agitation, followed by 7 rinses with PBS and one rinse with deionized water. Sections were mounted onto slides and allowed to dry for ~18 hrs, after which cover slips were mounted with Vectashield and sealed with nail polish.

Review submitted by:

Alipi Naydenov
Neurobiology and Behavior, Stella Lab
University of Washington