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mGluR1 alpha (Metabotropic Glutamate Receptor) Antibody

mGluR1 alpha (Metabotropic Glutamate Receptor) Antibody
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Price: $310.00
Product ID : 24426
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Description

The antibody has significant biotin-avidin HRP staining at a 1/300 - 1/500 dilution in rat thalamus, hippocampus and cortex. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.  The antibody is specific for the ~125k monomer and the ~250k mGluR1a dimer in Western blots of rat brain extracts.  Immunolabeling for mGluR1a is completely abolished by preadsorption with synthetic rat mGluR1a (1116-1130) at 5 µg per mL of diluted antibody.  Note:  If needed, low levels of glutaraldehyde (0.1 - 0.3%) may be used   in conjunction with paraformaldehyde.

Photo Description: IHC image of neurons staining for the mGluR1 alpha in the rat hippocampus (above) and western blot showing specificity of the antibody at 132 kDa (below). For IHC, the tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:500 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

mGluR1 #24426

Host: Rabbit

Quantity / Volume:  100 µL

State: Liquid

Reacts With: Rat

Availability: In Stock

Alternate Names:  mGluR1a; GRM1a; Gprc1a; Metabotropic glutamate receptor 1; Mglur1; G protein coupled receptor family C group 1 member A; glutamate receptor, metabotropic 1

Gene Symbol / ID, Accession #:  Grm1, 24414

 

Download Product Spec. SheetmGluR1a (Metabotropic Glutamate Receptor) Antibody

 

Journal References:


Dobi, Alice, et al. "Neural substrates for the distinct effects of presynaptic group III metabotropic glutamate receptors on extinction of contextual fear conditioning in mice." Neuropharmacology 66 (2013): 274-289. Tested in Mouse, PubMed ID: 22643400

Neves, Guilherme, et al. "The LIM homeodomain protein Lhx6 regulates maturation of interneurons and network excitability in the mammalian cortex." Cerebral Cortex 23.8 (2013): 1811-1823. Tested in Mouse, PubMed ID: 22710612

Ferraguti, Francesco, Luca Crepaldi, and Ferdinando Nicoletti. "Metabotropic glutamate 1 receptor: current concepts and perspectives." Pharmacological reviews 60.4 (2008): 536-581. Tested in Mouse, PubMed ID: 19112153

Tukker, John J., et al. "Cell type-specific tuning of hippocampal interneuron firing during gamma oscillations in vivo." The journal of neuroscience 27.31 (2007): 8184-8189. Tested in Rat, PubMed ID: 17670965

Namkoong, Jin, et al. "Metabotropic glutamate receptor 1 and glutamate signaling in human melanoma." Cancer research 67.5 (2007): 2298-2305. Tested in Human, PubMed ID: 17332361

Crepaldi, Luca, et al. "Transcriptional activators and repressors for the neuron-specific expression of a metabotropic glutamate receptor." Journal of Biological Chemistry 282.24 (2007): 17877-17889. Tested in Human and Mouse, PubMed ID: 17430891

Notenboom, Robbert GE, et al. "Up-regulation of hippocampal metabotropic glutamate receptor 5 in temporal lobe epilepsy patients." Brain 129.1 (2006): 96-107. Tested in Rat, PubMed ID: 16311265

Ferraguti, Francesco, et al. "Metabotropic glutamate receptor 8-expressing nerve terminals target subsets of GABAergic neurons in the hippocampus." The Journal of neuroscience 25.45 (2005): 10520-10536. Tested in Rat, PubMed ID: 16280590

Losonczy, Attila, et al. "Cell type dependence and variability in the short‐term plasticity of EPSCs in identified mouse hippocampal interneurones." The Journal of physiology 542.1 (2002): 193-210. Tested in Mouse, PubMed ID: 12096061

Karim, Farzana, Chia-Chuan Wang, and Robert W. Gereau. "Metabotropic glutamate receptor subtypes 1 and 5 are activators of extracellular signal-regulated kinase signaling required for inflammatory pain in mice." The Journal of Neuroscience 21.11 (2001): 3771-3779. Tested in Mouse, PubMed ID: 11356865

Bhave, G., et al. "Peripheral group I metabotropic glutamate receptors modulate nociception in mice." Nature neuroscience 4.4 (2001): 417-423. Tested in    Mouse, PubMed ID: 11276233

Alvarez, Francisco J., et al. "Differential distribution of metabotropic glutamate receptors 1a, 1b, and 5 in the rat spinal cord." Journal of Comparative Neurology 422.3 (2000): 464-487. Tested in Rat, PubMed ID: 10861520

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