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Gastrin Releasing Peptide (GRP) / Bombesin Antibody

Gastrin Releasing Peptide (GRP) / Bombesin Antibody
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Price: $275.00
Product ID : 20073
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Description

The ImmunoStar Gastrin Releasing Peptide antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat dorsal horn of spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/1,000-1/2,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of 1 mL of diluted antibody with 50 µg of bombesin.

Photo Description: IHC of at stomach staining for gastrin releasing peptide. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen and counterstained with hematoxylin

Host: Rabbit

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Reacts With:  Chick, Hamster, Human, Mouse, Newt, Pigeon, Quail, Rabbit, Ram (Sheep), Rat, Zacco Platypus (Minnow)

Availability: In Stock

Preabsorption Control: Available at irbiologicals, Catalog # IRCP069.  Sequence: Glp-Gln-Arg-Leu-Gly-Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH

Alternate Names:  BN; GRP-10; proGRP; preproGRP; gastrin-releasing peptide

Gene Symbol / ID, Accession #:  GRP,2922

 

Download Product Spec SheetGastrin Releasing Peptide (Bombesin) Antibody

 

Journal References:


Bombesin, Serotonergic potentiation of photic phase shifts: Examination of receptor contributions and early biochemical / molecular events, Victoria M. Smith, Kimberly Hagel, Michael C. Antle, J.Neuroscience, 4522(09, 1-51, 2009, mice


Akiyama, Tasuku, et al. "Roles of glutamate, substance P, and gastrin-releasing peptide as spinal neurotransmitters of histaminergic and nonhistaminergic itch." PAIN® 155.1 (2014): 80-92. Tested in Mouse, PubMed ID: 24041961

Fleming, Michael S., et al. "The majority of dorsal spinal cord gastrin releasing peptide is synthesized locally whereas neuromedin B is highly expressed in pain-and itch-sensing somatosensory neurons." Mol Pain 8.1 (2012): 52. Tested in Mouse, PubMed ID: 22776446

Tong Liu,1 Temugin Berta,1 Zhen-Zhong Xu,1 Chul-Kyu Park, Ling Zhang, Ning Lü, Qin Liu,Yang Liu, Yong-Jing Gao, Yen-Chin Liu, Qiufu Ma, Xinzhong Dong, and Ru-Rong Ji; TLR3 deficiency impairs spinal cord synaptic transmission, central sensitization, and pruritus in mice; The Journal of Clinical Investigation,122(6):2195-2207 (2012), Tested in Mouse, PubMed ID: 22565312

Popler, J., et al. "Familial neuroendocrine cell hyperplasia of infancy." Pediatric pulmonology 45.8 (2010): 749-755. Tested in Human, PubMed ID: 23787483

LeSauter, Joseph, et al. "Circadian trafficking of calbindin-ir in fibers of SCN neurons." Journal of biological rhythms 24.6 (2009): 488-496. Tested in Hamster, PubMed ID: 19926808

Roxanne Sterniczuk, Mark A. Colijn, Maria Nunez, Michael C. Antle; Investigating the role of substance P in photic responses of the circadian system: Individual and combined actions with gastrin-releasing peptide; Neuropharmacology,:1–9 (2009), Tested in Hamster, PubMed ID: 19540856

Liu, Qin, et al. "Sensory neuron-specific GPCR Mrgprs are itch receptors mediating chloroquine-induced pruritus." Cell 139.7 (2009): 1353-1365. Tested in Mouse, PubMed ID: 20004959

Victoria M. Smith, Kimberly Hagel, Michael C. Antle; "Serotonergic potentiation of photic phase shifts: Examination of receptor contributions and early biochemical / molecular events"; J.Neuroscience,4522(09:1-51) (2009), Tested in Mouse, PubMed ID: 19799970

Smith, V. M., et al. "Altered photic and non-photic phase shifts in 5-HT(1A) receptor knockout mice." Neuroscience 157.3 (2008): 513-523. Tested in Mouse, PubMed ID: 18930788

Yan-Gang Sun & Zhou-Feng Chen; A gastrin-releasing peptide receptor mediates; Nature,448:700-703 (2007), Tested in Mouse, PubMed ID: 17653196    

Gail H. Deutsch, Lisa R. Young, Robin R. Deterding, Leland L. Fan, Sharon D. Dell, Judy A. Bean; Diffuse Lung Disease in Young Children; Am J Respir Crit Care Med,176:1120-1128 (2007), Tested in Human, PubMed ID: 17885266

Miyakawa, Kazuko, et al. "ORL1 receptor-mediated down-regulation of mPER2 in the suprachiasmatic nucleus accelerates re-entrainment of the circadian clock following a shift in the environmental light/dark cycle." Neuropharmacology 52.3 (2007): 1055-1064. Tested in Mouse, PubMed ID: 17196226

Karatsoreos, Ilia N., et al. "Diurnal regulation of the gastrin‐releasing peptide receptor in the mouse circadian clock." European Journal of Neuroscience 23.4 (2006): 1047-1053. Tested in Mouse, PubMed ID: 16519669

Antle, Michael C., Joseph LeSauter, and Rae Silver. "Neurogenesis and ontogeny of specific cell phenotypes within the hamster suprachiasmatic nucleus." Developmental brain research 157.1 (2005): 8-18. Tested in Hamster, PubMed ID: 15939080

Deterding, Robin R., et al. "Persistent tachypnea of infancy is associated with neuroendocrine cell hyperplasia." Pediatric pulmonology 40.2 (2005): 157-165. Tested in Human, PubMed ID: 15965897

Au, N. H. C., et al. "Evaluation of immunohistochemical markers in non‐small cell lung cancer by unsupervised hierarchical clustering analysis: a tissue microarray study of 284 cases and 18 markers." The Journal of pathology 204.1 (2004): 101-109. Tested in Human, PubMed ID: 15307143

Lee, J. H., et al. "Immunohistochemical study of the gastrointestinal endocrine cells in the Korean aucha perch." Journal of fish biology 65.1 (2004): 170-181.

Ku, S. K., J. H. Lee, and H. S. Lee. "Immunohistochemical study on the endocrine cells in gut of the stomachless teleost, Zacco platypus (Cyprinidae)." Anatomia, histologia, embryologia 33.4 (2004): 212-219. Tested in Zacco Platypus (Minnow), PubMed ID: 15239812

Karatsoreos, Ilia N., et al. "Phenotype matters: identification of light-responsive cells in the mouse suprachiasmatic nucleus." The Journal of neuroscience 24.1 (2004): 68-75. Tested in Mouse, PubMed ID: 14715939

Aalto, Yan, et al. "Does bombesin-like peptide mediate radiation-induced anorexia and satiety?." Acta Oncologica 38.8 (1999): 1099-1102. PubMed ID: 10665769

Trandaburu, T., S. Syed Ali, and I. Trandaburu. "Granin proteins (chromogranin A and secretogranin II C23-3 and C26-3) in the intestine of reptiles." Annals of Anatomy-Anatomischer Anzeiger 181.3 (1999): 261-268. PubMed ID: 10363108

Dixon, J. S., P. Y. P. Jen, and J. A. Gosling. "A double‐label immunohistochemical study of intramural ganglia from the human male urinary bladder neck." Journal of anatomy 190.1 (1997): 125-134. Tested in Human, PubMed ID: 9034888

Wang, Dashou, Herman Yeger, and Ernest Cutz. "Expression of gastrin-releasing peptide receptor gene in developing lung." American journal of respiratory cell and molecular biology 14.5 (1996): 409-416. PubMed ID: 8624245

Luckensmeyer, Greta B., and Janet R. Keast. "Immunohistochemical characterisation of viscerofugal neurons projecting to the inferior mesenteric and major pelvic ganglia in the male rat." Journal of the autonomic nervous system 61.1 (1996): 6-16. Tested in Rat, PubMed ID: 8912248

Wang, Dashou, et al. "NADPH-oxidase and a hydrogen peroxide-sensitive K+ channel may function as an oxygen sensor complex in airway chemoreceptors and small cell lung carcinoma cell lines." Proceedings of the National Academy of Sciences 93.23 (1996): 13182-13187. Tested in Human and Rabbit, PubMed ID: 8917565

Jen, P. Y. P., J. S. Dixon, and J. A. Gosling. "Development of peptide‐containing nerves in the human fetal vas deferens and seminal vesicle." British journal of urology 75.3 (1995): 378-385. Tested in Human, PubMed ID: 7735805

Berk, Mitchell L., and Stacy E. Smith. "Local and commissural neuropeptide‐containing projections of the nucleus of the solitary tract to the dorsal vagal complex in the pigeon." Journal of Comparative Neurology 347.3 (1994): 369-396. Tested in Pigeon, PubMed ID: 7529775

Keast, J. R., and H-C. Chiam. "Selective association of nerve fibres immunoreactive for substance P or bombesin with putative cholinergic neurons of the male rat major pelvic ganglion." Cell and tissue research 278.3 (1994): 589-594. Tested in Rat, PubMed ID: 7531621

Martinez, Alfredo, et al. "Localization of amidating enzymes (PAM) in rat gastrointestinal tract." Journal of Histochemistry & Cytochemistry 41.11 (1993): 1617-1622. Tested in Rat, PubMed ID: 8409369

Linnoila, R. Ilona, et al. "Long‐term selective culture of hamster pulmonary endocrine cells." The Anatomical Record 236.1 (1993): 231-240. Tested in Hamster, PubMed ID: 8389531

Westlund, K. N., et al. "Effects of nerve growth factor and acetyl-L-carnitine arginyl amide on the human neuronal line HCN-1A." International journal of developmental neuroscience 10.5 (1992): 361-373. Tested in Human, PubMed ID: 1283485

Houben, Herlinde, and Carl Denef. "Evidence for the Presence of Gastrin-Releasing Peptide Immunoreactivity in Rat Anterior Pituitary Corticotrophs and Lactotrophs, AtT20 Cells, and GH3 Cells: Failure to Demonstrate Participation in Local Control of Hormone Release*." Endocrinology 128.6 (1991): 3208-3218. Tested in Rat, PubMed ID: 1645264

Multidifferentiated, Protooncogenes Induce. "Human Bronchial Epithelial Cells Transformed by the c." CANCER RESEARCH 51 (1991): 3793-3801. Tested in Human, PubMed ID: 1712250

Globus, Morton, and Paul Alles. "A search for immunoreactive substance P and other neural peptides in the limb regenerate of the newt Notophthalmus viridescens." Journal of Experimental Zoology 254.2 (1990): 165-176. Tested in Newt, PubMed ID: 1693394

Hernanz, Angel. "Characterization and distribution of bombesin-like peptides in the rat brain and gastrointestinal tract." Biochemistry and Cell Biology 68.9 (1990): 1142-1145. Tested in Rat, PubMed ID: 2257118

Sithigorngul, Paisarn, Antony OW Stretton, and Cynthia Cowden. "Neuropeptide diversity in Ascaris: an immunocytochemical study." Journal of Comparative Neurology 294.3 (1990): 362-376. PubMed ID: 2341616

Vittoria, A., et al. "Serotonin-, somatostatin-and chromogranin A-containing cells of the urethro-prostatic complex in the sheep. An immunocytochemical and immunofluorescent study." Journal of anatomy 171 (1990): 169. Tested in Ram / Sheep, PubMed ID: 1981998

Stein, Brenda, and Ann Andrew. "Differentiation of endocrine cells in chick allantoic epithelium combined with pancreatic mesenchyme." Cell differentiation and development 26.3 (1989): 173-180. Tested in Chick, PubMed ID: 2475227

Montuenga, L. M., et al. "Ultrastructure and immunocytochemistry of endocrine cells in the midgut of the desert locust, Schistocerca gregaria (Forskal)." Cell and tissue research 258.3 (1989): 577-583.  

Rizk, Sherif L., et al. "Elevated creatine phosphokinase MB in a patient with neuroendocrine carcinoma of the colon—Evidence for a tumor marker." Diseases of the Colon & Rectum 31.4 (1988): 318-322. PubMed ID: 3359901

Favara, Blaise E., and Harold Moores. "Anitschkow nuclear structure: a study of pediatric hearts." Fetal and Pediatric Pathology 7.2 (1987): 151-164. PubMed ID: 2821523

Chott, Andreas, et al. "Neuron specific enolase demonstration in the diagnosis of a solid-cystic (papillary cystic) tumour of the pancreas." Virchows Archiv A 410.5 (1987): 397-402. PubMed ID: 3103322

Kramer, B. E. V. E. R. L. E. Y., et al. "The effect of pancreatic mesenchyme on the differentiation of endocrine cells from gastric endoderm." Development 100.4 (1987): 661-671. Tested in Quail, PubMed ID: 3443051

Müller-Hermelink, H. K., M. Marino, and G. Palestro. "Pathology of thymic epithelial tumors." The Human Thymus. Springer Berlin Heidelberg, 1986. 207-268.

Warren, William H., et al. "Paragangliomas of the head and neck: ultrastructural and immunohistochemical analysis." Ultrastructural pathology 8.4 (1985): 333-343.

Marchevsky, A. M., and J. Kleinerman. "Quantitative and immunocytochemical studies of APUD cells in airways and gut." Lung 161.1 (1983): 67-76. PubMed ID: 6188931

Andrew, Ann, Beverley Kramer, and B. B. Rawdon. "The embryonic origin of endocrine cells of the gastrointestinal tract." General and comparative endocrinology 47.2 (1982): 249-265. PubMed ID: 7095418

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