GABA (gamma-Aminobutyric Acid) Antibody, diluted 1:100

GABA (gamma-Aminobutyric Acid) Antibody, diluted 1:100
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Price: $130.00
Product ID : 20095
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The antibody produces strong labeling at dilutions of 1/120 - 1/150 (which is equivalent to 1/12,000 - 1/15,000) using biotin-streptavidin/HRP technique in rat cerebellum and thalamus. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

Immunolabeling is completely abolished by preadsorption with excess GABA/BSA conjugate.

Photo Description: IHC image of neurons staining for GABA in the rat thalamus (above) and the rat cerebellum (below left, low magnification; below right, high magnification). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:20000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen and hemotoxylin counterstain in the rat thalamus.

GABA cerebellum  GABA cerebellum 2

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Cat, Fish, Frog, Monkey, Mouse, Rat, Turtle

Availability: In Stock


Download Product Spec. SheetGABA (gamma-Aminobutyric Acid) Antibody, diluted 1:100

Journal References:

Cytological organization of the central gelatinosa in the turtle spinal cord; Trujillo-Cenóz O, Fernández A, Radmilovich M, Reali C, Russo RE; J Comp Neurol.,502(2):291-308, 2007, Tested in Turtle, IFC, Cites Diasorin AKA ImmunoStar, PubMed ID: 17348014

Citations with the GABA Antibody # 20094 (Same antibody but not pre-diluted):

Anatomy of the posterior caudal lobe of the cerebellum and the eminentia granularis posterior in a mormyrid fish; Campbell HR, Meek J, Zhang J, Bell CC.; J Comp Neurol.,502(5):714-35, 2007, Tested in Fish, Cites Diasorin AKA ImmunoStar, PubMed ID: 17436286

Disruption of the Gene Encoding SF-1 Alters the Distribution of Hypothalamic Neuronal Phenotypes; Tammy L. Dellovade, Morag Young,Elizabeth P. Ross,Rachael Henderson, Kathleen Caron,Keith Parker,Stuart A. Tobet; Journal of comparative neurology, 423(4):579-89, 2000, Tested in Mouse, Cites Diasorin AKA ImmunoStar, PubMed ID: 10880989

Morphological analysis of the mormyrid cerebellum using immunohistochemistry, with emphasis on the unusual neuronal organization of the valvula; Meek J, Yang JY, Han VZ, Bell CC; J Comp Neurol.,510(4):396-421, 2008, Tested in Fish, Cites Diasorin AKA ImmunoStar, PubMed ID: 18663756

Special Relationship of gamma-Aminobutyric Acid to the Ventromedial Nucleus of the Hypothalamus During Embryonic Development; Stuart A. Tobet,  Rachel G. Henderson, Paul J. Whiting, Werner Sieghart; The Journal of Neuroscience,405(1):88-98, 1999, Tested in Mouse, Cites Diasorin AKA ImmunoStar, PubMed ID: 10022198

Laminar distribution of GABA-immunoreactive neurons and processes in area 18 of the cat.; Matsubara JA, Nance DM, Cynader MS.; Brain Research Bulletin,18(1):121-126, 1987, Tested in Cat, Cites ImmunoNuclear AKA ImmunoStar, PubMed ID: 3828838

Changes in Hippocampal Circuitry after Pilocarpine-Induced Seizures as Revealed by Opioid Receptor Distribution and Activation; Bausch SB, Chavkin C; The Journal of Neuroscience,17(1):477-492, 1997, Tested in Rat, Cites Incstar AKA ImmunoStar, PubMed ID: 8987772

Opioid Enhancement of Clacium Oscillations and Burst Events Involving NMDA Receptors and L-Type Calcium Channels in Cultured Hippocampal Neurons; Przewlocki R, Parsons KL, Sweeney DD; The Journal of Neuroscience,19(22):9705-9715, 1999, Tested in Rat, Cites Incstar AKA ImmunoStar, PubMed ID: 10559380

L-Type Calcium Channels Mediate Calcium Oscillations in Early Postnatal Purkinje Neurons; Liljelund JG, Netzeband, DL Gruol; The Journal of Neuroscience,20(19):7394-7403, 2000, Tested in Rat, Cites Incstar AKA ImmunoStar, PubMed ID: 11007898

Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system.; Reynolds BA, Weiss S.; Science,255(5052):1707-1710, 1992, Tested in Mouse, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 1553558

Involvement of Distinct Pioneer Neurons in the Formation of Layer-Specific Connections in the Hippocampus; Super H, Martinez A, Del Rio JA; The Journal of Neuroscience, 18(12):4616-4626, 1998, Tested in Mouse, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 9614236

Transplanted Neuroblasts Differentiate Appropriately into Projection Neurons with Correct Neurotransmitter and Receptor Phenotype in Neocortex Undergoing Targeted Projection Neuron Degeneration; Shin JJ, Fricker-Gates RA,  Perez FA; The Journal of Neuroscience, 20(19):7404-7416, 2000, Tested in Mouse, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 11007899

Dependence of GABAergic Synaptic Areas on the Interneuron Type and Target Size; Kubota Y, Kawaguchi Y; The Journal of Neuroscience, 20(1):375-386, 2000, Tested in Rat, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 10627614

Progestin receptor is transiently expressed perinatally in neurons of the rat isocortex; López V, Wagner CK.; J Comp Neurol., 512(1):124-39, 2009, Tested in Rat, Cites Incstar AKA ImmunoStar, PubMed ID: 18973223

Roles for c-Aminobutyric Acid in the Development of the Paraventricular Nucleus of the Hypothalamus; ; JCN,518:2710-2728, 2010, Tested in Mouse, PubMed ID: 20506472

Functional and molecular clues reveal precursor-like cells and immature neurones in the turtle spinal cord; Russo, Fernández, Reali, Radmilovich, Trujillo-Cenóz; J Physiol, 560.3:831-838, 2004, Tested in Turtle, PubMed ID: 15331672

Ptf1a triggers GABAergic neuronal cell fates in; Jean-Philippe Dullin, Morgane Locker, Mélodie Robach, Kristine A Henningfeld, Karine Parain, Solomon Afelik, Tomas Pieler and Muriel Perron; BMC Developmental Biology,7:110, 2007, Tested in Frog, PubMed ID: 17910758

Changes in Rx1 and Pax6 activity; Norann A. Zaghloul, Sally A. Moody; Molecular Vision,13:86-95, 2007, Tested in Frog, PubMed ID: 17277735

Generation of Functional Eyes from Pluripotent; Andrea S. Viczian, Eduardo C. Solessio, Yung Lyou, Michael E. Zuber; PLoS Biol,:, 2009, Tested in Frog, PubMed ID: 19688031

Cone Degeneration Following Rod Ablation in a Reversible Model of Retinal Degeneration; Rene Y. Choi, Gustav A. Engbretson, Eduardo C. Solessio, Georgette A. Jones, Adam Coughlin, Ilija Aleksic, Michael E. Zuber; Invest Ophthalmol Vis Sci. ,52:364-373, 2011, Tested in Frog, PubMed ID: 20720220

Pathways for emotions and attention converge on the thalamic reticular nucleus in primates; Zikopoulos B, Barbas H; J Neurosci,32(15):5338-50, 2012, Tested in Monkey, PubMed ID: 22496579

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