GABA (gamma-Aminobutyric Acid) Antibody, diluted 1:100

GABA (gamma-Aminobutyric Acid) Antibody, diluted 1:100
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Price: $130.00
Product ID : 20095
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The antibody produces strong labeling at dilutions of 1/120 - 1/150 (which is equivalent to 1/12,000 - 1/15,000) using biotin-streptavidin/HRP technique in rat cerebellum and thalamus. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

Immunolabeling is completely abolished by preadsorption with excess GABA/BSA conjugate.

Photo Description: IHC image of neurons staining for GABA in the rat thalamus (above) and the rat cerebellum (below left, low magnification; below right, high magnification). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:20000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen and hemotoxylin counterstain in the rat thalamus.

GABA cerebellum  GABA cerebellum 2

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Alligator, Armadillo, Bat, Bathynomus Doederleini (Crustacean/Isopod), Bird, Caenorhabditis Elegans Nematode (Roundworm), Cat, Drosophila (Fly), Drosophila Melanogaster (Fly), Ferret, Fish, Frog, Frog, Frog (Xenopus Laevis), Frog (Xenopus Tectum), Gerbil, Guinea Pig, Hamster, Human, Kitten (Cat), Monkey, Moth, Mouse, Parakeet, Pigeon, Pleurobranchaea Japonica (Sea Slug), Rabbit, Racoon, Rat, Slug, Snail, Turtle, Zebra Finch (Bird)

Availability: In Stock


Download Product Spec. SheetGABA (gamma-Aminobutyric Acid) Antibody, diluted 1:100

Journal References:

Cytological organization of the central gelatinosa in the turtle spinal cord; Trujillo-Cenóz O, Fernández A, Radmilovich M, Reali C, Russo RE; J Comp Neurol.,502(2):291-308, 2007, Tested in Turtle, IFC, Cites Diasorin AKA ImmunoStar, PubMed ID: 17348014

Citations with the GABA Antibody # 20094 (Same antibody but not pre-diluted):

Anatomy of the posterior caudal lobe of the cerebellum and the eminentia granularis posterior in a mormyrid fish; Campbell HR, Meek J, Zhang J, Bell CC.; J Comp Neurol.,502(5):714-35, 2007, Tested in Fish, Cites Diasorin AKA ImmunoStar, PubMed ID: 17436286

Disruption of the Gene Encoding SF-1 Alters the Distribution of Hypothalamic Neuronal Phenotypes; Tammy L. Dellovade, Morag Young,Elizabeth P. Ross,Rachael Henderson, Kathleen Caron,Keith Parker,Stuart A. Tobet; Journal of comparative neurology, 423(4):579-89, 2000, Tested in Mouse, Cites Diasorin AKA ImmunoStar, PubMed ID: 10880989

Morphological analysis of the mormyrid cerebellum using immunohistochemistry, with emphasis on the unusual neuronal organization of the valvula; Meek J, Yang JY, Han VZ, Bell CC; J Comp Neurol.,510(4):396-421, 2008, Tested in Fish, Cites Diasorin AKA ImmunoStar, PubMed ID: 18663756

Special Relationship of gamma-Aminobutyric Acid to the Ventromedial Nucleus of the Hypothalamus During Embryonic Development; Stuart A. Tobet,  Rachel G. Henderson, Paul J. Whiting, Werner Sieghart; The Journal of Neuroscience,405(1):88-98, 1999, Tested in Mouse, Cites Diasorin AKA ImmunoStar, PubMed ID: 10022198

Laminar distribution of GABA-immunoreactive neurons and processes in area 18 of the cat.; Matsubara JA, Nance DM, Cynader MS.; Brain Research Bulletin,18(1):121-126, 1987, Tested in Cat, Cites ImmunoNuclear AKA ImmunoStar, PubMed ID: 3828838

Changes in Hippocampal Circuitry after Pilocarpine-Induced Seizures as Revealed by Opioid Receptor Distribution and Activation; Bausch SB, Chavkin C; The Journal of Neuroscience,17(1):477-492, 1997, Tested in Rat, Cites Incstar AKA ImmunoStar, PubMed ID: 8987772

Opioid Enhancement of Clacium Oscillations and Burst Events Involving NMDA Receptors and L-Type Calcium Channels in Cultured Hippocampal Neurons; Przewlocki R, Parsons KL, Sweeney DD; The Journal of Neuroscience,19(22):9705-9715, 1999, Tested in Rat, Cites Incstar AKA ImmunoStar, PubMed ID: 10559380

L-Type Calcium Channels Mediate Calcium Oscillations in Early Postnatal Purkinje Neurons; Liljelund JG, Netzeband, DL Gruol; The Journal of Neuroscience,20(19):7394-7403, 2000, Tested in Rat, Cites Incstar AKA ImmunoStar, PubMed ID: 11007898

Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system.; Reynolds BA, Weiss S.; Science,255(5052):1707-1710, 1992, Tested in Mouse, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 1553558

Involvement of Distinct Pioneer Neurons in the Formation of Layer-Specific Connections in the Hippocampus; Super H, Martinez A, Del Rio JA; The Journal of Neuroscience, 18(12):4616-4626, 1998, Tested in Mouse, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 9614236

Transplanted Neuroblasts Differentiate Appropriately into Projection Neurons with Correct Neurotransmitter and Receptor Phenotype in Neocortex Undergoing Targeted Projection Neuron Degeneration; Shin JJ, Fricker-Gates RA,  Perez FA; The Journal of Neuroscience, 20(19):7404-7416, 2000, Tested in Mouse, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 11007899

Dependence of GABAergic Synaptic Areas on the Interneuron Type and Target Size; Kubota Y, Kawaguchi Y; The Journal of Neuroscience, 20(1):375-386, 2000, Tested in Rat, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 10627614

Progestin receptor is transiently expressed perinatally in neurons of the rat isocortex; López V, Wagner CK.; J Comp Neurol., 512(1):124-39, 2009, Tested in Rat, Cites Incstar AKA ImmunoStar, PubMed ID: 18973223

Roles for c-Aminobutyric Acid in the Development of the Paraventricular Nucleus of the Hypothalamus; ; JCN,518:2710-2728, 2010, Tested in Mouse, PubMed ID: 20506472

Functional and molecular clues reveal precursor-like cells and immature neurones in the turtle spinal cord; Russo, Fernández, Reali, Radmilovich, Trujillo-Cenóz; J Physiol, 560.3:831-838, 2004, Tested in Turtle, PubMed ID: 15331672

Ptf1a triggers GABAergic neuronal cell fates in; Jean-Philippe Dullin, Morgane Locker, Mélodie Robach, Kristine A Henningfeld, Karine Parain, Solomon Afelik, Tomas Pieler and Muriel Perron; BMC Developmental Biology,7:110, 2007, Tested in Frog, PubMed ID: 17910758

Changes in Rx1 and Pax6 activity; Norann A. Zaghloul, Sally A. Moody; Molecular Vision,13:86-95, 2007, Tested in Frog, PubMed ID: 17277735

Generation of Functional Eyes from Pluripotent; Andrea S. Viczian, Eduardo C. Solessio, Yung Lyou, Michael E. Zuber; PLoS Biol,:, 2009, Tested in Frog, PubMed ID: 19688031

Cone Degeneration Following Rod Ablation in a Reversible Model of Retinal Degeneration; Rene Y. Choi, Gustav A. Engbretson, Eduardo C. Solessio, Georgette A. Jones, Adam Coughlin, Ilija Aleksic, Michael E. Zuber; Invest Ophthalmol Vis Sci. ,52:364-373, 2011, Tested in Frog, PubMed ID: 20720220

Pathways for emotions and attention converge on the thalamic reticular nucleus in primates; Zikopoulos B, Barbas H; J Neurosci,32(15):5338-50, 2012, Tested in Monkey, PubMed ID: 22496579

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