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DBH (Dopamine-beta-Hydroxylase) Antibody

DBH positive neurons in rat brainstem
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Price: $375.00
Product ID : 22806
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Description

The antibody has a proven strong biotin-streptavidin/HRP staining at a 1/2000 - 1/4000 dilution in rat brainstem, cerebellum and adrenal medulla. 

The above image is of DBH positive neurons in rat brainstem,  vibratome sectioned, ABC / DAB detection.

Using Western blot of purified DBH the antiserum detects a triplet at approximately 72-74 kD. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

Photo Description: IHC image of neurons staining for DBH in the rat brainstem. The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:4000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Species Reactivity: Bird, Bird (Starling), Cat, Ferret, Finch, Guinea Pig, Hamster, Hatchetfish, Human, Ilyanassa Obsoleta (Sea Snail), Monkey, Mouse, Pig, Quail, Rat, Sparrow, Starling, Steer (Cattle), Turkey, Turtle, Zebra Finch (Bird)

Availability: In Stock

Alternate Names:  DBM; DOPO; Dopamine beta-monooxygenase

Gene Symbol / ID, Accession #:  DBH,280758

 

Download Product Spec. SheetDBH (Dopamine-beta-Hydroxylase) Antibody

 

Journal References:


Central Neural Distribution of Immunoreactive Fos and CRH; Exp Neurol. 2007, Jun; 205(2):485-500, Tested in Rat, PubMed ID: 17462630


Bethea, Cynthia L., et al. "Hypothalamic KISS1 Expression, Gonadotrophin‐Releasing Hormone and Neurotransmitter Innervation Vary with Stress and Sensitivity in Macaques." Journal of neuroendocrinology 26.5 (2014): 267-281. Tested in Monkey, PubMed ID: 24617839

Wade, Juli, et al. "Norepinephrine inhibition in juvenile male zebra finches modulates adult song quality." Brain research bulletin 90 (2013): 132-136. Tested in Zebra Finch (Bird), PubMed ID: 23160069

Brosnahan, Amanda J., et al. "Norepinephrine potentiates proinflammatory responses of human vaginal epithelial cells." Journal of neuroimmunology 259.1 (2013): 8-16. Tested in Human, PubMed ID: 23571017

He, Gonghao, et al. "Arrhythmogenic effect of sympathetic histamine in mouse hearts subjected to acute ischemia." Molecular Medicine 18.1 (2012). Tested in Mouse, PubMed ID: 21989948

Pawlisch, Benjamin A., et al. "Behavioral indices of breeding readiness in female European starlings correlate with immunolabeling for catecholamine markers in brain areas involved in sexual motivation." General and comparative endocrinology 179.3 (2012): 359-368. Tested in Bird (Starling), PubMed ID: 22999823

Thompson, V. B., et al. "Prenatal exposure to MDMA alters noradrenergic neurodevelopment in the rat." Neurotoxicology and teratology 34.1 (2012): 206-213. Tested in Rat, PubMed ID: 21978916

Vaughan, C. H., Y. B. Shrestha, and T. J. Bartness. "Characterization of a novel melanocortin receptor-containing node in the SNS outflow circuitry to brown adipose tissue involved in thermogenesis." Brain research 1411 (2011): 17-27. Tested in Hamster, PubMed ID: 21802070

Matragrano, Lisa L., et al. "Estradiol‐dependent catecholaminergic innervation of auditory areas in a seasonally breeding songbird." European Journal of Neuroscience 34.3 (2011): 416-425. Tested in Rat, PubMed ID: 21714815

Stubbusch, Jutta, et al. "Generation of the tamoxifen‐inducible DBH‐Cre transgenic mouse line DBH‐CT." genesis 49.12 (2011): 935-941. Tested in Mouse, PubMed ID: 21634003

Zaccone, Giacomo, et al. "Immunolocalization of neurotransmitter-synthesizing enzymes and neuropeptides with associated receptors in the photophores of the hatchetfish,Argyropelecus hemigymnus Cocco, 1829." Acta histochemica 113.4 (2011): 457-464. Tested in Hatchetfish, PubMed ID: 20546867

Cantor, Thomas L., and Ping Gao. "Methods for differentiating and monitoring parathyroid and bone status related diseases." U.S. Patent No. 7,943,323. 17 May 2011.

Colline Poirier, Tiny Boumans, Michiel Vellema, Geert De Groof, Thierry D. Charlier, Marleen Verhoye, Annemie Van der Linden, Jacques Balthazart; Own Song Selectivity in the Songbird Auditory Pathway: Suppression by Norepinephrine; PLoS ONE,6:1-6 (2011), Tested in Zebra Finch (Bird), PubMed ID: 21625389

Torsten Bullmann, Wolfgang Härtig, Max Holzer, Thomas Arendt; Expression of the embryonal isoform (0N/3R) of the microtubule-associated protein tau in the adult rat central nervous system; The Journal of Comparative Neurology,518(13):2538-53 (2010), Tested in Rat, PubMed ID: 20503426    

Zhang, Xiaoli, et al. "Pontine norepinephrine defects inMecp2- null mice involve deficient expression of dopamine β-hydroxylase but not a loss of catecholaminergic neurons." Biochemical and biophysical research communications 394.2 (2010): 285-290. Tested in Mouse, PubMed ID: 20193660

Clarke, Gwenaëlle L., et al. "β-adrenoceptor blockers increase cardiac sympathetic innervation by inhibiting autoreceptor suppression of axon growth." The Journal of Neuroscience 30.37 (2010): 12446-12454. Tested in Rat, PubMed ID: 20844139

Tri Wahyu Pangestiningsiha,d, Anita Hendricksonb, Koeswinarning Sigitc, Dondin Sajuthic, Nurhidayatc, Douglas M. Bowdene; "Development of the area postrema: An immunohistochemical study in the macaque"; Brain Res. 2009 Jul 14;1280:23-32 (2009), Tested in Monkey, PubMed ID: 19460361

Wernli, Gwenaelle, et al. "Macrophage depletion suppresses sympathetic hyperinnervation following myocardial infarction." Basic research in cardiology 104.6 (2009): 681-693. Tested in Rat,    PubMed ID: 19437062

Clarke, Gwenaelle Laetitia. "Regulation of sympathetic plasticity in the heart." (2009).

Kang, S. W., et al. "Serotonergic and Catecholaminergic Interactions with Co‐Localised Dopamine‐Melatonin Neurones in the Hypothalamus of the Female Turkey." Journal of neuroendocrinology 21.1 (2009): 10-19. Tested in Turkey, PubMed ID: 19094089

Hoard, Jennifer L., et al. "Cholinergic neurons of mouse intrinsic cardiac ganglia contain noradrenergic enzymes, norepinephrine transporters, and the neurotrophin receptors tropomyosin-related kinase A and p75." Neuroscience 156.1 (2008): 129-142. Tested in Mouse, PubMed ID: 18674600

Sarah A. Heimovics and Lauren V. Riters; Evidence that dopamine within motivation and song control brain regions regulates birdsong context-dependently; Physiol Behav.,95:258-266 (2008), Tested in Starling, PubMed ID: 18619478

Sockman KW, Salvante KG; The integration of song environment by catecholaminergic Systems Innervating the Auditory Telencephalon of Adult Female European Starlings; Develop Neurobiol,68:656-668 (2008), Tested in Bird, PubMed ID: 18278799

Balan, Paul Ernsberger, and Musa A. Haxhiu. "Allergic lung inflammation affects central noradrenergic control of cholinergic outflow to the airways in ferrets." J Appl Physiol 103 (2007): 2095-2104. Tested in Ferret, PubMed ID: 17872402

Lisa D. Schmidt, Yonghong Xie, Mark Lyte, Lucy Vulchanova, and David R. Brown; Autonomic Neurotransmitters Modulate Immunoglobulin A Secretion in Porcine Colonic Mucosa; J Neuroimmunol.,185:20-28 (2007), Tested in Pig, PubMed ID: 17320195

Dvoryanchikov, Gennady, Seth M. Tomchik, and Nirupa Chaudhari. "Biogenic amine synthesis and uptake in rodent taste buds." Journal of Comparative Neurology 505.3 (2007): 302-313. Tested in Mouse, PubMed ID: 17879273

Meredith M. LeBlanc, Christopher T. Goode, Elizabeth A. MacDougall-Shackleton, Donna L. Maney; Estradiol modulates brainstem catecholaminergic cell groups and projections to the auditory forebrain in a female songbird; Brain Research,1171:98-103 (2007), Tested in Sparrow, PubMed ID: 17764666

Andrews, Melissa R., and Dennis J. Stelzner. "Evaluation of olfactory ensheathing and Schwann cells after implantation into a dorsal injury of adult rat spinal cord." Journal of neurotrauma 24.11 (2007): 1773-1792. Tested in Rat, PubMed ID: 18001205

Riters, Lauren V., and Benjamin A. Pawlisch. "Evidence that norepinephrine influences responses to male courtship song and activity within song control regions and the ventromedial nucleus of the hypothalamus in female European starlings." Brain research 1149 (2007): 127-140. Tested in Starling, PubMed ID: 17379191

Castelino, Christina B., Bettina Diekamp, and Gregory F. Ball. "Noradrenergic projections to the song control nucleus area X of the medial striatum in male zebra finches (Taeniopygia guttata)." Journal of Comparative Neurology 502.4 (2007): 544-562. Tested in Finch, PubMed ID: 17394158

Brock, J. A., D. J. Handelsman, and J. R. Keast. "Postnatal androgen deprivation dissociates the development of smooth muscle innervation from functional neurotransmission in mouse vas deferens." The Journal of physiology 581.2 (2007): 665-678. Tested in Mouse, PubMed ID: 17379637

Chen, Chunsheng, et al. "Mucosally-directed adrenergic nerves and sympathomimetic drugs enhance non-intimate adherence of  Escherichia coli  O157: H7 to porcine cecum and colon." European journal of pharmacology 539.1 (2006): 116-124. Tested in Mouse and Pig, PubMed ID: 16687138

Flores, Juan A., et al. "Role for dopamine neurons of the rostral linear nucleus and periaqueductal gray in the rewarding and sensitizing properties of heroin." Neuropsychopharmacology 31.7 (2006): 1475-1488. Tested in Rat, PubMed ID: 16292327

Castelino, Christina B., and Gregory F. Ball. "A role for norepinephrine in the regulation of context‐dependent ZENK expression in male zebra finches (Taeniopygia guttata)." European Journal of Neuroscience 21.7 (2005): 1962-1972. Tested in Finch, PubMed ID: 15869489

Walton, M. E., et al. "The mesocortical dopamine projection to anterior cingulate cortex plays no role in guiding effort-related decisions." Behavioral neuroscience 119.1 (2005): 323. Tested in Rat, PubMed ID: 15727537

Yagi, Hideshi, et al. "Vlgr1 knockout mice show audiogenic seizure susceptibility." Journal of neurochemistry 92.1 (2005): 191-202. Tested in Mouse, PubMed ID: 19539720

Green, Lyte, Chen, Xie, Casey, Kulkarni-Narla, Vulchanova, Brown; Adrenergic modulation of Escherichia coli O157:H7 adherence to the colonic mucosa; Am J Physiol Gastrointest Liver Physiol ,287:G1238-G1246 (2004), Tested in Pig, PubMed ID: 15534374

Meijs MFL, Timmers L, Pearse DP, Tresco PA, et al; Basic Fibroblast Growth Factor Promotes Neuronal Survival but Not Behavioral Recovery in the Transected and Schwann Cell Implanted Rat Thoracic Spinal Cord; Journal of Neurotrauma,21 (#10):1415-1430 (2004), Tested in Rat, PubMed ID: 15672632

Antonopoulos, John, et al. "Noradrenergic innervation of the developing and mature septal area of the rat." Journal of Comparative Neurology 476.1 (2004): 80-90. Tested in Rat, PubMed ID: 15236468

Dickinson, Amanda JG, and Roger P. Croll. "Development of the larval nervous system of the gastropod Ilyanassa obsoleta." Journal of Comparative Neurology 466.2 (2003): 197-218. Tested in Ilyanassa Obsoleta (Sea Snail), PubMed ID: 14528448

Schumm, Michael A., et al. "Direct cell–cell contact required for neurotrophic effect of chromaffin cells on neural progenitor cells." Developmental brain research 146.1 (2003): 1-13. PubMed ID: 14643006

Gaszner, Balázs, and Tamás Kozicz. "Interaction between catecholaminergic terminals and urocortinergic neurons in the Edinger-Westphal nucleus in the rat." Brain research 989.1 (2003): 117-121. Tested in Rat, PubMed ID: 14519518

Cantor, Tom, Patty Glowacki, and Steve Scheibel. "Large differences between commercial intact PTH assays." J Am Soc Nephrol 14 (2003): 688A.

Garcia, C., et al. "The influence of specific noradrenergic and serotonergic lesions on the expression of hippocampal brain-derived neurotrophic factor transcripts following voluntary physical activity." Neuroscience 119.3 (2003): 721-732. PubMed ID: 12809693

Behrens, Edward G., Brett R. Schofield, and Ann M. Thompson. "Aminergic projections to cochlear nucleus via descending auditory pathways." Brain research 955.1 (2002): 34-44. PubMed ID: 12419519

SÁNCHEZ-CAMACHO, C. R. I. S. T. I. N. A., OSCAR MARÍN, and AGUSTÍN GONZÁLEZ. "Distribution and origin of the catecholaminergic innervation in the amphibian mesencephalic tectum." Visual neuroscience 19.03 (2002): 321-333. PubMed ID: 12392181

Schumm, Michael A., et al. "Enhanced viability and neuronal differentiation of neural progenitors by chromaffin cell co-culture." Developmental brain research 137.2 (2002): 115-125. PubMed ID: 12220703

Kawasaki, Hiroshi, et al. "Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity." Proceedings of the National Academy of Sciences 99.3 (2002): 1580-1585. Tested in Monkey, PubMed ID: 11818560

Latsari, Maria, et al. "Noradrenergic innervation of the developing and mature visual and motor cortex of the rat brain: a light and electron microscopic immunocytochemical analysis." Journal of Comparative Neurology 445.2 (2002): 145-158. Tested in Rat, PubMed ID: 11891659

Takami, Toshihiro, et al. "Schwann cell but not olfactory ensheathing glia transplants improve hindlimb locomotor performance in the moderately contused adult rat thoracic spinal cord." The Journal of neuroscience 22.15 (2002): 6670-6681. Tested in Rat, PubMed ID: 12151546

Mundorf, Michelle L., et al. "Catecholamine release and uptake in the mouse prefrontal cortex." Journal of neurochemistry 79.1 (2001): 130-142. Tested in    Mouse, PubMed ID: 11595765

Espejo, Emilio Fernandez, et al. "Functional regeneration in a rat Parkinson's model after intrastriatal grafts of glial cell line-derived neurotrophic factor and transforming growth factor β1-expressing extra-adrenal chromaffin cells of the zuckerkandl's organ." The Journal of Neuroscience 21.24 (2001): 9888-9895. Tested in Rat, PubMed ID: 11739596

Stewart, Graham R., Roland N. Perry, and Denis J. Wright. "Occurrence of dopamine in Panagrellus redivivus and Meloidogyne incognita." Nematology 3.8 (2001): 843-848.

Abrahamson, Eric E., and Robert Y. Moore. "Suprachiasmatic nucleus in the mouse: retinal innervation, intrinsic organization and efferent projections." Brain research 916.1 (2001): 172-191. Tested in Mouse, PubMed ID: 11597605

Jen, P. Y. P., J. S. Dixon, and J. A. Gosling. "Colocalisation of neuropeptides, nitric oxide synthase and immunomarkers for catecholamines in nerve fibres of the adult human vas deferens." Journal of anatomy 195.4 (1999): 481-489. Tested in Human, PubMed ID: 10634688

Haidan, A., et al. "Basal Steroidogenic Activity of Adrenocortical Cells Is Increased 10-Fold by Coculture with Chromaffin Cells 1." Endocrinology 139.2 (1998): 772-780. Tested in Steer (Cattle), PubMed ID: 9449652

Ramón-Cueto, Almudena, et al. "Long-distance axonal regeneration in the transected adult rat spinal cord is promoted by olfactory ensheathing glia transplants." The Journal of neuroscience 18.10 (1998): 3803-3815. Tested in Rat, PubMed ID: 9570810

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Product Reviews

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Reviewed by user
02/27/2014 - 02:02:15 PM
Very happy with the results
We used rabbit anti-bovine DBH (#22806) in an ICC Zenk protocol with sectioned zebra finch brain tissue and were very happy with the results. The antibody provided clear staining with minimal background. The antibody clearly labeled neuron bodies as well as tracks.

Our protocol followed Sockman & Salvante 2007. Zebra finch brains were fixed using 4% paraformaldehyde, saturated in 30% sucrose and stored at -80. Brains were sectioned at 40 um using a cryostat and sections were stored in cryoprotectant at -20.

Primary

1. 3x5 min wash in PBS
2. 15 min incubation in 0.1% sodium borohydride
3. 3x5 min wash in PBS
4. 30 min incubation in 0.5% H2O2 in PBS
5. 3x5 min was in 0.3% PBST
6. 1 hr incubation in 20% normal goat serum
7. 15 min incubation in Aviden solution
8. 3x5 min wash in 0.3% PBST
9. 15 min incubation in Biotin
10. 3x5 min wash in 0.3% PBST
11. 48 hr incubation at 4˚C at 1:16,000 in PBSTN

Secondary

1. 1 hour incubation in secondary antibody at RT
2. Visualize using ABC kit and DAB
3. Dehydrate and coverslip

Submitted by:

Kendra Sewall, PhD
Assistant Professor
Biological Sciences, Virginia Tech
Reviewed by user
11/15/2012 - 11:33:39 AM
Antibody Worked Very Well
We tried the rabbit anti-dopamine-beta-hydroxylase antibody in ferret tissues, more specifically on brainstem tissues. Ferrets were perfused with saline followed by 4% paraformaldehyde and postfixed for 12 hours.

We used 1:500 dilution of DBH antibody and stained with Alexa 594 as a secondary antibody. The citation of the antibody has been published in the following article:

Allergic lung inflammation affects central noradrenergic control of cholinergic outflow to the airways in ferrets. Wilson CG, Akhter S, Mayer CA, Kc P, Balan KV, Ernsberger P, Haxhiu MA. J Appl Physiol. 2007 Dec;103(6):2095-104. Epub 2007 Sep 13. PMID:17872402[PubMed]

Submitted by:

Prabha Kc, Ph.D.
Assistant Professor
Case Western Reserve University
Department of Pediatrics
Cleveland, OH
Reviewed by user
09/14/2012 - 11:22:04 AM
Detected small fibers with very little background
Animals were briefly anesthetized with pentobarbital (200mg/kg) followed by transcardial perfusion with 200 ml heparinized saline and 500 ml Zamboni fixative. Brains were then removed and stored in 30% sucrose until further processing.

IHC

Brains were frozen and cut at 50 µm thick sections using a cryostat.

Free-floating sections were first washed with PBS and then pretreated with a solution of 50% methanol, 0.3% hydrogen peroxide in PBS for 1 hour.

Blocking Solution: 1% triton-X 100, 2% fetal bovine serum in .1 M PBS for at least 1 hour at room temperature.

Dilutent for primary and secondary antibodies: 0.5% triton-X 100, 2% fetal bovine serum in .1 M PBS at

Primary dilution 1:5000 overnight at 4°C
Secondary Alexa 488 (Life Technologies) 1:500 in dilutent for 1 hour

Sections were washed thoroughly in .1 M PBS following detection, coverslipped and mounted with Vectashield mounting medium (Vector Labs)


This antibody detected small fibers within the cortex, central amygdala and various nuclei of the thalamus and hypothalamus with very little background.

Note: The time which animals spend under anesthesia will affect the level of expression in the thalamus/hypothalamus regions.

Review submitted by:

Vanessa M. Kainz
Research Associate
Department of Anesthesia and Critical Care Beth Israel Deaconess Medical Center
Reviewed by user
04/23/2012 - 02:32:02 PM
High quality antibody
I used the DBH antibody and the results showed very nice staining and no background.

Zebra finch brain tissue
Flash frozen and stored at -80 degrees
Sectioned at 20 um on cryostat and placed on superfrost plus slides.
Slides stored at -80 degrees until processing.
4% paraformaldehyde -15 min @ RT (room temperature)
3 X 5 min washes in 1X PBS @ RT
0.9% H2O2 in methanol-15 min @RT
3 X 5 min washes in 1XPBS @ RT
10% normal goat serum - 1 hour @ RT

Primary antibody incubation: overnight @ 4 degrees
1 ul/ml antibody
10% normal goat serum
1XPBS-TritonX

Secondary antibody - 90 min @ RT

Visualize using ABC kit (Vector) and DAB
Dehydrate and coverslip.


Review submitted by:

Juli S. Wade, PhD
Yu-Ping Tang, PhD
Neuroscience Program
Michigan State University