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DBH (Dopamine-beta-Hydroxylase) Antibody

DBH positive neurons in rat brainstem
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Price: $375.00
Product ID : 22806
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Description

The antibody has a proven strong indirect immunofluorescence at 1/400 - 1/800 and biotin-streptavidin/HRP staining at a 1/2000 - 1/4000 dilution in rat brainstem, cerebellum and adrenal medulla. 

The above image is of DBH positive neurons in rat brainstem,  vibratome sectioned, ABC / DAB detection.

Using Western blot of purified DBH the antiserum detects a triplet at approximately 72-74 kD. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.


Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Species Reactivity: Bird, Ferret, Monkey, Mouse, Pig, Rat, Sparrow, Starling, Zebra Finch

Availability: In Stock

Alternate Names:  DBM; DOPO; Dopamine beta-monooxygenase

Gene Symbol / ID, Accession #:  DBH,280758

 

Download Product Spec. SheetDBH (Dopamine-beta-Hydroxylase) Antibody

 

Journal References:


Functional Regeneration in a Rat Parkinson's Model after Intrastriatal Grafts of Glial Cell Line-Derived Neurotrophic Factor and Transforming Growth Factor beta 1-Expressing Extra-Adrenal Chromaffin Cells of the Zuckerkandl's Organ; Espejo EF, Gonzalez-Albo MC, Moraes JP, Banoua FEl, Flores JA, Caraballo I ; Journal of Neuroscience,21(24):9888-9895, 2001, Tested in Rat, Cites Diasorin AKA ImmunoStar, PubMed ID: 11739596

Expression of the embryonal isoform (0N/3R) of the microtubule-associated protein tau in the adult rat central nervous system; Torsten Bullmann, Wolfgang Härtig, Max Holzer, Thomas Arendt; The Journal of Comparative Neurology,518(13):2538-53, 2010, Tested in Rat, IFC, Cites Diasorin AKA ImmunoStar, PubMed ID: 20503426

The integration of song environment by catecholaminergic Systems Innervating the Auditory Telencephalon of Adult Female European Starlings; Sockman KW, Salvante KG; Develop Neurobiol,68:656-668, 2008, Tested in Bird , PubMed ID: 18278799

Autonomic Neurotransmitters Modulate Immunoglobulin A Secretion in Porcine Colonic Mucosa; Lisa D. Schmidt, Yonghong Xie, Mark Lyte, Lucy Vulchanova, and David R. Brown; J Neuroimmunol., 185:20-28, 2007, Tested in Pig, PubMed ID: 17320195

Central Neural Distribution of Immunoreactive Fos and CRH; Exp Neurol. 2007, Jun; 205(2):485-500, Tested in Rat, PubMed ID: 17462630

Estradiol modulates brainstem catecholaminergic cell groups and projections to the auditory forebrain in a female songbird; Meredith M. LeBlanc, Christopher T. Goode, Elizabeth A. MacDougall-Shackleton, Donna L. Maney; Brain Research,1171:98-103, 2007, Tested in Sparrow, PubMed ID: 17764666

Estradiol-dependent catecholaminergic innervation of auditory areas in a seasonally breeding songbird; Matragrano LL, Sanford SE, Salvante KG, Sockman KW, Maney DL.; European Journal of Neuroscience,34 (3):416-425, 2011, Tested in Sparrow, PubMed ID: 21714815

Evidence that dopamine within motivation and song control brain regions regulates birdsong context-dependently; Sarah A. Heimovics and Lauren V. Riters; Physiol Behav.,95:258-266, 2008, Tested in Starling, PubMed ID: 18619478

A role for norepinephrine in the regulation of context-dependent ZENK expression in male zebra
finches (Taeniopygia guttata); Christina B. Castelino and Gregory F. Ball; European Journal of Neuroscience,21:1962–1972, 2005, Tested in Zebra Finch, PubMed ID: 15869489

Allergic lung inflammation affects central noradrenergic control of cholinergic outflow to the airways in ferrets; Wilson CG, Akhter S, Mayer CA, Kc P, Balan KV, Ernsberger P, Haxhiu MA.; J Appl Physiol.,103 (6):2095-2104, 2007, Tested in Ferret, PubMed ID: 17872402

Development of the area postrema: An immunohistochemical study in the macaque; Brain Research,1280:23–32, Tested in Monkey, PubMed ID: 19460361

Arrhythmogenic Effect of Sympathetic Histamine in Mouse Hearts Subjected to Acute Ischemia; Gonghao He, Jing Hu, Teng Li, Xue Ma, Jingru Meng, Min Jia, Jun Lu, Hiroshi Ohtsu, Zhong Chen, and Xiaoxing Luo; Mol Med,18(1):1-9, 2012, Tested in Mouse, PubMed ID: 21989948

Adrenergic modulation of Escherichia coli O157:H7 adherence to the colonic mucosa; Green, Lyte, Chen, Xie, Casey, Kulkarni-Narla, Vulchanova, Brown; Am J Physiol Gastrointest Liver Physiol 287:G1238-G1246, 2004, Tested in Pig, PubMed ID: 15534374

Basic Fibroblast Growth Factor Promotes Neuronal Survival but Not Behavioral Recovery in the Transected and Schwann Cell Implanted Rat Thoracic Spinal Cord; Meijs MFL, Timmers L, Pearse DP, Tresco PA, et al; Journal of Neurotrauma,21 (#10):1415-1430, 2004, Tested in Rat, PubMed ID: 15672632

Sympathetic Hyperinnervation and Inflammatory Cell NGF Synthesis Following Myocardial Infarction in Rats; Wohaib Hasan, Abdi Jama, Timothy Donohue, Gwenaelle Wernli, Gregory Onyszchuk, Baraa Al-Hafez, Mehmet Bilgen, and Peter G. Smith,; Brain Res.,1124:142-154, 2006, Tested in Rat, PubMed ID: 17084822

Own Song Selectivity in the Songbird Auditory Pathway: Suppression by Norepinephrine; Colline Poirier, Tiny Boumans, Michiel Vellema, Geert De Groof, Thierry D. Charlier, Marleen Verhoye, Annemie Van der Linden, Jacques Balthazart; PLoS ONE,6:1-6, 2011, Tested in Zebra Finch, PubMed ID: 21625389

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Product Reviews

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Reviewed by user
02/27/2014 - 02:02:15 PM
Very happy with the results
We used rabbit anti-bovine DBH (#22806) in an ICC Zenk protocol with sectioned zebra finch brain tissue and were very happy with the results. The antibody provided clear staining with minimal background. The antibody clearly labeled neuron bodies as well as tracks.

Our protocol followed Sockman & Salvante 2007. Zebra finch brains were fixed using 4% paraformaldehyde, saturated in 30% sucrose and stored at -80. Brains were sectioned at 40 um using a cryostat and sections were stored in cryoprotectant at -20.

Primary

1. 3x5 min wash in PBS
2. 15 min incubation in 0.1% sodium borohydride
3. 3x5 min wash in PBS
4. 30 min incubation in 0.5% H2O2 in PBS
5. 3x5 min was in 0.3% PBST
6. 1 hr incubation in 20% normal goat serum
7. 15 min incubation in Aviden solution
8. 3x5 min wash in 0.3% PBST
9. 15 min incubation in Biotin
10. 3x5 min wash in 0.3% PBST
11. 48 hr incubation at 4˚C at 1:16,000 in PBSTN

Secondary

1. 1 hour incubation in secondary antibody at RT
2. Visualize using ABC kit and DAB
3. Dehydrate and coverslip

Submitted by:

Kendra Sewall, PhD
Assistant Professor
Biological Sciences, Virginia Tech
Reviewed by user
11/15/2012 - 11:33:39 AM
Antibody Worked Very Well
We tried the rabbit anti-dopamine-beta-hydroxylase antibody in ferret tissues, more specifically on brainstem tissues. Ferrets were perfused with saline followed by 4% paraformaldehyde and postfixed for 12 hours.

We used 1:500 dilution of DBH antibody and stained with Alexa 594 as a secondary antibody. The citation of the antibody has been published in the following article:

Allergic lung inflammation affects central noradrenergic control of cholinergic outflow to the airways in ferrets. Wilson CG, Akhter S, Mayer CA, Kc P, Balan KV, Ernsberger P, Haxhiu MA. J Appl Physiol. 2007 Dec;103(6):2095-104. Epub 2007 Sep 13. PMID:17872402[PubMed]

Submitted by:

Prabha Kc, Ph.D.
Assistant Professor
Case Western Reserve University
Department of Pediatrics
Cleveland, OH
Reviewed by user
09/14/2012 - 11:22:04 AM
Detected small fibers with very little background
Animals were briefly anesthetized with pentobarbital (200mg/kg) followed by transcardial perfusion with 200 ml heparinized saline and 500 ml Zamboni fixative. Brains were then removed and stored in 30% sucrose until further processing.

IHC

Brains were frozen and cut at 50 µm thick sections using a cryostat.

Free-floating sections were first washed with PBS and then pretreated with a solution of 50% methanol, 0.3% hydrogen peroxide in PBS for 1 hour.

Blocking Solution: 1% triton-X 100, 2% fetal bovine serum in .1 M PBS for at least 1 hour at room temperature.

Dilutent for primary and secondary antibodies: 0.5% triton-X 100, 2% fetal bovine serum in .1 M PBS at

Primary dilution 1:5000 overnight at 4°C
Secondary Alexa 488 (Life Technologies) 1:500 in dilutent for 1 hour

Sections were washed thoroughly in .1 M PBS following detection, coverslipped and mounted with Vectashield mounting medium (Vector Labs)


This antibody detected small fibers within the cortex, central amygdala and various nuclei of the thalamus and hypothalamus with very little background.

Note: The time which animals spend under anesthesia will affect the level of expression in the thalamus/hypothalamus regions.

Review submitted by:

Vanessa M. Kainz
Research Associate
Department of Anesthesia and Critical Care Beth Israel Deaconess Medical Center
Reviewed by user
04/23/2012 - 02:32:02 PM
High quality antibody
I used the DBH antibody and the results showed very nice staining and no background.

Zebra finch brain tissue
Flash frozen and stored at -80 degrees
Sectioned at 20 um on cryostat and placed on superfrost plus slides.
Slides stored at -80 degrees until processing.
4% paraformaldehyde -15 min @ RT (room temperature)
3 X 5 min washes in 1X PBS @ RT
0.9% H2O2 in methanol-15 min @RT
3 X 5 min washes in 1XPBS @ RT
10% normal goat serum - 1 hour @ RT

Primary antibody incubation: overnight @ 4 degrees
1 ul/ml antibody
10% normal goat serum
1XPBS-TritonX

Secondary antibody - 90 min @ RT

Visualize using ABC kit (Vector) and DAB
Dehydrate and coverslip.


Review submitted by:

Juli S. Wade, PhD
Yu-Ping Tang, PhD
Neuroscience Program
Michigan State University