C-FOS Antibody

For induction of c-fos protein activity rats were injected with 1.0 ml of 1.5 M NaCl per 100 grams of body weight. Negative control rats were injected with the same volume of normal saline. The ImmunoStar c-fos antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat paraventricular nucleus and supraoptic nucleus using indirect immunofluorescent and biotin/avidin-HRP techniques. No labeling was seen in negative control rats. Recommended primary dilutions for these methods are 1/200-1/400 in PBS/0.3% Triton X-100 - FITC Technique and 1/4000-1/6000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique.



Specificity of the antiserum was demonstrated by blockage of staining in experimental rats by omission of c-fos antibody or by substitution of antibody pre-incubated with synthetic peptide or the conjugate. Immunoblot analysis of mediobasal hypothalamus showed a single band of approximately 55-60 kD.

Host: Rabbit

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Reacts With: Rat

Predicted to React With: Bovine, Cat, Fish, Hamster, Human, Monkey, Mouse, Pig

Availability: In Stock


Download Product Spec Sheet: C-FOS Antibody

Journal References:

1. Coping strategies in male and female rats exposed to multiple stressors., Campbell T, Lin S, DeVries C, Lambert K, Physiology & Behavior, 78, 495-504, 2003.

2. Differences In The Suprachiasmatic Nucleus And Lower Subparaventricular Zone Of Diurnal And Nocturnal Rodents, M. D. Schwartz,A A. A. Nuneza,B And L. Smale, Neuroscience, 127, 13-23, 2004, Tested in Rat.