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Beta-Endorphin Antibody

Beta-Endorphin Antibody
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Price: $210.00
Product ID : 20063
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Description

The antibody produces a strong biotin-streptavidin/HRP staining at a 1/1000 - 1/2000 dilution in rat anterior pituitary.

Staining is completely eliminated by pretreatment of the diluted antibody with 10-6 M of Beta-Endorphin. Pre-adsorption of the diluted antibody with 10-6M of the following substances had no effect on Beta-Endorphin labeling: methionine enkephalin, leucine enkaphalin, dynorphin A, dynorphin B, gamma-endorphin, alpha-endorphin, ACTH and alpha-melanocyte stimulating hormone.

Photo Description: IHC images of beta-endorphin staining in the rat anterior pituitary with FITC-conjugated secondary antibody (above) and with standard biotin-streptavidin/HRP and DAB chromogen (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. 

Endorphin pituitary #20063

Host: Rabbit

Quantity / Volume: 100 µL

State: Lyophilized

Availability: In Stock

Reacts With: Mouse, Rat

Preabsorption Control: Available at Sigma Aldrich, Catalog # E1142

Alternate Names:  Endorfin-beta; Endorphin-beta; proopiomelanocortin

Gene Symbol / ID, Accession #:  POMC,5443

 

Download Product Spec Sheet: Beta-Endorphin Antibody

 

Journal References:


Borgquist, Amanda, et al. "Gonadal Steroids Differentially Modulate the Actions of Orphanin FQ/Nociceptin at A Physiologically Relevant Circuit Controlling Female Sexual Receptivity." Journal of neuroendocrinology 26.5 (2014): 329-340., Borgquist A, Rivas VM, Kachani M, Sinchak K, Wagner EJ., Tested in Mouse, IHC method, PubMed ID: 24617903

Borgquist, Amanda, et al. "Estradiol Negatively Modulates the Pleiotropic Actions of Orphanin FQ/Nociceptin at Proopiomelanocortin Synapses." Neuroendocrinology 98.1 (2013): 60-72., Borgquist A, Kachani M, Tavitian N, Sinchak K, Wagner EJ. IHC method

Gerbe, François, et al. "Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium." The Journal of cell biology 192.5 (2011): 767-780. Gerbe F, van Es JH, Makrini L, Brulin B, Mellitzer G, Robine S, Romagnolo B,Shroyer NF, Bourgaux JF, Pignodel C, Clevers H, Jay P. Tested in Human and Mouse, PubMed ID: 21383077

GASTROENTEROLOGY,137:598-606, (2009) "Release of Endogenous Opioids From Duodenal Enteroendocrine Cells
Requires Trpm5", Zaza Kokrashvili, Deniliz Rodriguez, Valeriya Yevshayeva, Hang Zhou, Robert F. Margolskee, And Bedrich Mosinger, Tested in Mouse, IF method, PubMed ID: 19272386

Stagg, Nicola Jane. "Reversal of Neuropathic Pain with Exercise is Mediated by Endogenous Opioids." (2007). Stagg NJ, Mata HP, Ibrahim MM, Henriksen EJ, Porreca F, Vanderah TW, PhilipMalan T Jr., Tested in Rat, PubMed ID: 21386701

Sakharkar, A. J., et al. "Reproduction Phase‐Related Expression of β‐Endorphin‐Like Immunoreactivity in the Nucleus Lateralis Tuberis of the Female Indian Major Carp Cirrhinus mrigala: Correlation with the Luteinising Hormone Cells‐Ovary Axis." Journal of neuroendocrinology 18.5 (2006): 319-329. Sakharkar AJ, Singru PS, Mazumdar M, Subhedar N., Tested in Carp, ICC method, PubMed ID: 16629830

Hughes, Sharon, et al. "Upregulation of the pro-opiomelanocortin gene in motoneurones after acrylamide administration in mice." Neuroscience letters 357.3 (2004): 232-234., Hughes S, Child T, Simpson MG, Smith ME., Tested in Mouse, PubMed ID: 15003292

Chronwall, Bibie M., et al. "Glial somatostatin-14 expression in the rat pituitary intermediate lobe: a possible neurotrophic function during development?." International Journal of Developmental Neuroscience 18.7 (2000): 685-692., Chronwall BM, Sands SA, Cummings KC 3rd, Schwartz JP., Tested in Rat, PubMed ID: 10978847

Clifton, V. L., et al. "Corticotropin-releasing hormone and proopiomelanocortin-derived peptides are present in human myometrium." Journal of Clinical Endocrinology & Metabolism 83.10 (1998): 3716-3721., Clifton VL, Telfer JF, Thompson AJ, Cameron IT, Teoh TG, Lye SJ, Challis JR.    Tested in Human, IHC method, PubMed ID: 9768689

Perna, Gian Piero, et al. "Plasma β-endorphin response to exercise in patients with congestive heart failure." CHEST Journal 111.1 (1997): 19-22.    Perna GP, Modoni S, Valle G, Stanislao M, Loperfido F., Tested in Human, PubMed ID: 8995987

CESANO, A. "L. PINESSI, I. RAINERO, T. DE GENNARO, A. VIOLANTE, D. CASSANO, L. MATERA,* AND." Annals of the New York Academy of Sciences 650 (1992): 351., PubMed ID: 1318663

Vethamany‐Globus, Swani, and Gregory Michener. "Localization of β‐endorphin‐like immunoreactivity in the nervous system of the adult newt, Notophthalmus viridescens." Journal of Experimental Zoology 262.2 (1992): 172-179., Tested in Newt, IHC method, PubMed ID: 1583460

Valevski, A., et al. "Neuroendocrine response to trihexyphenidyl in depressed patients." Clinical neuropharmacology 15.1 (1992): 27-33.    Valevski A, Gil-Ad I, Modai I, Apter A, Tyano S, Laron Z, Weizman A., PubMed ID: 1576596

Weizman, Ronit, et al. "Immunoreactive [beta]-Endorphin, Cortisol, and Growth Hormone Plasma Levels in Obsessive-Compulsive Disorder." Clinical neuropharmacology 13.4 (1990): 297-302., Weizman R, Gil-Ad I, Hermesh H, Munitz H, Laron Z., PubMed ID: 2208181

Roy III, Benjamin F. "Method for detecting antibodies against neuropeptides and drugs in human body fluid." U.S. Patent No. 4,868,107. 19 Sep. 1989. Tested in Human

WEIZMAN, RONIT, et al. "Low Plasma Immunoreactive β‐Endorphin Levels in Autism." Journal of the American Academy of Child & Adolescent Psychiatry 27.4 (1988): 430-433. Weizman R, Gil-Ad I, Dick J, Tyano S, Szekely GA, Laron Z.    

Weizman, Abraham, et al. "Plasma Cortisol, Prolactin, Growth Hormone, and Immunoreactive [beta]-Endorphin Response to Fenfluramine Challenge in Depressed Patients." Clinical neuropharmacology 11.3 (1988): 250-256. Weizman A, Mark M, Gil-Ad I, Tyano S, Laron Z. PubMed ID: 2969772    

Bhathena, Sam J., et al. "Decreased plasma enkephalins in copper deficiency in man." The American journal of clinical nutrition 43.1 (1986): 42-46.    Decreased plasma enkephalins in copper deficiency in man. Bhathena SJ, Recant L, Voyles NR, Timmers KI, Reiser S, Smith JC Jr, Powell AS. Tested in Human, PubMed ID: 2934971

Hinshaw, Lerner B., et al. "Evaluation of naloxone for therapy of Escherichia coli shock: Species differences." Archives of Surgery 119.12 (1984): 1410. Hinshaw LB, Beller BK, Chang AC, Flournoy DJ, Lahti RA, Passey RB, Archer LT. PubMed ID: 6391420

Saad, Mohammed F., et al. "Occult Cushing's disease presenting with acute psychosis." The American journal of medicine 76.4 (1984): 759-766., IHC method, PubMed ID: 6711582

COHN, DAVID V., et al. "Selective Localization of the Parathyroid Secretory Protein-I/Adrenal Medulla Chromogranin A Protein Family in a Wide Variety of Endocrine Cells of the Rat*." Endocrinology 114.6 (1984): 1963-1974. David V. Cohn, James J. Elting, Mary Frick, Robert Elde, Tested in Rat, IFC method, PubMed ID: 6233131

Rama Sastry, B. V., et al. "Enkephalin-and substance P-like immunoreactivities of mammalian sperm and accessory sex glands." Biochemical pharmacology 31.21 (1982): 3519-3522., Tested in Human and Rat, PubMed ID: 6184060

Basso, N., et al. "Effect of ceruletide on pituitary-hypothalamic peptides and on emotion in man." Peptides 2 (1981): 71-75. Basso N, Materia A, D'Intinosante V, Ginaldi A, Pona V, Reilly P, Ruggeri S, Fioravanti M., PubMed ID: 6178096

Fex, Jörgen, and Richard A. Altschuler. "Enkephalin-like immunoreactivity of olivocochlear nerve fibers in cochlea of guinea pig and cat." Proceedings of the National Academy of Sciences 78.2 (1981): 1255-1259., Tested in Cat and Guinea Pig, IF method, PubMed ID: 7015329

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