Beta-Endorphin Antibody

Beta-Endorphin Antibody
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Price: $210.00
Product ID : 20063
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The antibody produces a strong biotin-streptavidin/HRP staining at a 1/1000 - 1/2000 dilution in rat anterior pituitary.

Staining is completely eliminated by pretreatment of the diluted antibody with 10-6 M of Beta-Endorphin. Pre-adsorption of the diluted antibody with 10-6M of the following substances had no effect on Beta-Endorphin labeling: methionine enkephalin, leucine enkaphalin, dynorphin A, dynorphin B, gamma-endorphin, alpha-endorphin, ACTH and alpha-melanocyte stimulating hormone.

Photo Description: IHC images of beta-endorphin staining in the rat anterior pituitary with FITC-conjugated secondary antibody (above) and with standard biotin-streptavidin/HRP and DAB chromogen (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. 

Endorphin pituitary #20063

Host: Rabbit

Quantity / Volume: 100 µL

State: Lyophilized

Availability: In Stock

Reacts With: Carp, Cat, Guinea Pig, Human, Mouse, Newt, Rat

Preabsorption Control: Available at Sigma Aldrich, Catalog # E1142

Alternate Names:  Endorfin-beta; Endorphin-beta; proopiomelanocortin

Gene Symbol / ID, Accession #:  POMC,5443


Download Product Spec Sheet: Beta-Endorphin Antibody


Journal References:

Borgquist, Amanda, et al. "Gonadal Steroids Differentially Modulate the Actions of Orphanin FQ/Nociceptin at A Physiologically Relevant Circuit Controlling Female Sexual Receptivity." Journal of neuroendocrinology 26.5 (2014): 329-340., Borgquist A, Rivas VM, Kachani M, Sinchak K, Wagner EJ., Tested in Mouse, IHC method, PubMed ID: 24617903

Borgquist, Amanda, et al. "Estradiol Negatively Modulates the Pleiotropic Actions of Orphanin FQ/Nociceptin at Proopiomelanocortin Synapses." Neuroendocrinology 98.1 (2013): 60-72., Borgquist A, Kachani M, Tavitian N, Sinchak K, Wagner EJ. IHC method

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Hughes, Sharon, et al. "Upregulation of the pro-opiomelanocortin gene in motoneurones after acrylamide administration in mice." Neuroscience letters 357.3 (2004): 232-234., Hughes S, Child T, Simpson MG, Smith ME., Tested in Mouse, PubMed ID: 15003292

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Weizman, Ronit, et al. "Immunoreactive [beta]-Endorphin, Cortisol, and Growth Hormone Plasma Levels in Obsessive-Compulsive Disorder." Clinical neuropharmacology 13.4 (1990): 297-302., Weizman R, Gil-Ad I, Hermesh H, Munitz H, Laron Z., PubMed ID: 2208181

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WEIZMAN, RONIT, et al. "Low Plasma Immunoreactive β‐Endorphin Levels in Autism." Journal of the American Academy of Child & Adolescent Psychiatry 27.4 (1988): 430-433. Weizman R, Gil-Ad I, Dick J, Tyano S, Szekely GA, Laron Z.    

Weizman, Abraham, et al. "Plasma Cortisol, Prolactin, Growth Hormone, and Immunoreactive [beta]-Endorphin Response to Fenfluramine Challenge in Depressed Patients." Clinical neuropharmacology 11.3 (1988): 250-256. Weizman A, Mark M, Gil-Ad I, Tyano S, Laron Z. PubMed ID: 2969772    

Bhathena, Sam J., et al. "Decreased plasma enkephalins in copper deficiency in man." The American journal of clinical nutrition 43.1 (1986): 42-46.    Decreased plasma enkephalins in copper deficiency in man. Bhathena SJ, Recant L, Voyles NR, Timmers KI, Reiser S, Smith JC Jr, Powell AS. Tested in Human, PubMed ID: 2934971

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Saad, Mohammed F., et al. "Occult Cushing's disease presenting with acute psychosis." The American journal of medicine 76.4 (1984): 759-766., IHC method, PubMed ID: 6711582

COHN, DAVID V., et al. "Selective Localization of the Parathyroid Secretory Protein-I/Adrenal Medulla Chromogranin A Protein Family in a Wide Variety of Endocrine Cells of the Rat*." Endocrinology 114.6 (1984): 1963-1974. David V. Cohn, James J. Elting, Mary Frick, Robert Elde, Tested in Rat, IFC method, PubMed ID: 6233131

Rama Sastry, B. V., et al. "Enkephalin-and substance P-like immunoreactivities of mammalian sperm and accessory sex glands." Biochemical pharmacology 31.21 (1982): 3519-3522., Tested in Human and Rat, PubMed ID: 6184060

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Fex, Jörgen, and Richard A. Altschuler. "Enkephalin-like immunoreactivity of olivocochlear nerve fibers in cochlea of guinea pig and cat." Proceedings of the National Academy of Sciences 78.2 (1981): 1255-1259., Tested in Cat and Guinea Pig, IF method, PubMed ID: 7015329

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