Alpha-MSH (Melanocyte Stimulating Hormone) Antibody

Alpha-MSH (Melanocyte Stimulating Hormone) Antibody
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Price: $250.00
Product ID : 20074
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The ImmunoStar alpha melanocyte stimulating hormone antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pituitary using biotin/avidin-HRP techniques.

Recommended primary dilution is 1/4000-1/6000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 ug/mL of alpha-MSH.

Photo Description: IHC image of neurons staining for MSH in the rat hypothalamus (above) and rat pituitary with nickel preparation (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. 

MSH pituitary #20074

Host: Rabbit

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Reacts With: Pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Trout

Availability: In Stock

Alternate Names:  POMC; proopiomelanocortin 

Gene Symbol / ID, Accession #:  POMC,5443


Download Product Spec Sheet: Alpha-MSH (Melanocyte Stimulating Hormone) Antibody


Journal References:

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Food deprivation-induced changes in body fat mobilization after neonatal monosodium glutamatetreatment; Claudia Leitner and Timothy J. Bartness; American Journal of Physiology, 294:R775-R783, 2008, Tested in Hamster, PubMed ID: 18094070

Borgquist, Amanda, et al. "Gonadal Steroids Differentially Modulate the Actions of Orphanin FQ/Nociceptin at A Physiologically Relevant Circuit Controlling Female Sexual Receptivity." Journal of neuroendocrinology 26.5 (2014): 329-340. Tested in Mouse, PubMed ID: 24617903

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Borgquist, Amanda, et al. "Estradiol Negatively Modulates the Pleiotropic Actions of Orphanin FQ/Nociceptin at Proopiomelanocortin Synapses." Neuroendocrinology 98.1 (2013): 60-72.

Kawabe, Tetsuya, Kazumi Kawabe, and Hreday N. Sapru. "Effect of barodenervation on cardiovascular responses elicited from the hypothalamic arcuate nucleus of the rat." PloS one 7.12 (2012): e53111.    Tested in    Rat, PubMed ID: 23300873

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Claudia Leitner, Timothy J. Bartness; Acute brown adipose tissue temperature response to cold in monosodium glutamate-treated Siberian hamsters; Brain Research,1292:3 8 – 5 1 (2009), Tested in    Hamster, PubMed ID: 19643091

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Kwon-Yul Ryu, Jacob C. Garza, Xin-Yun Lu, Gregory S. Barsh, and Ron R. Kopito; Hypothalamic neurodegeneration and adult-onset obesity in mice lacking the Ubb polyubiquitin gene; PNAS,105:4016-4021 (2008), Tested in Mouse, PubMed ID: 18299572

Jennie Ho, Jeremy M. Cox, and Edward J. Wagner; Cannabinoid-Induced Hyperphagia: Correlation With Inhibition Of Proopiomelanocortin Neurons?; Physiol Behav.,92(3):507-519 (2007), Tested in Guinea Pig, PubMed ID: 17532014

Koban, Michael, Wei Wei Le, and Gloria E. Hoffman. "Changes in hypothalamic corticotropin-releasing hormone, neuropeptide Y, and proopiomelanocortin gene expression during chronic rapid eye movement sleep deprivation of rats." Endocrinology 147.1 (2006): 421-431. Tested in    Rat, PubMed ID: 16210372

Nguyen, Que Huong, and Edward J. Wagner. "Estrogen differentially modulates the cannabinoid-induced presynaptic inhibition of amino acid neurotransmission in proopiomelanocortin neurons of the arcuate nucleus." Neuroendocrinology 84.2 (2006): 123-137. Tested in Guinea Pig, PubMed ID: 17106183

Bjorn Meister, Burcak Gomuc¸ Elisabet Suarez, Yuko Ishii, Katrin Durr and Linda Gillberg; Hypothalamic proopiomelanocortin (POMC) neurons; European Journal of Neuroscience,24:2731-2740 (2006), Tested in Rat, PubMed ID: 17156199

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Allison Wanting Xu, Christopher B. Kaelin, Kiyoshi Takeda, Shizuo Akira, Michael W. Schwartz, and Gregory S. Barsh; PI3K integrates the action of insulin and leptin on hypothalamic neurons; The Journal of Clinical Investigation,115:951-958 (2005), Tested in Mouse, PubMed ID: 15761497

Zheng, Patterson, Phifer, Berthoud; Brainstem melanocortinergic modulation of meal size and identification of hypothalamic POMC projections; Am J Physiol Regul Integr Comp Physiol ,869:(1-29) (2004), Tested in Rat, PubMed ID: 15746303

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Lloyd, Ricardo V., et al. "Corticotroph (Basophil) invasion of the pars nervosa in the human pituitary: Localization of proopiomelanocortin peptides, galanin and peptidylglycine α-amidating monooxygenase-like immunoreactivities." Endocrine Pathology 4.2 (1993): 86-94.

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Frost‐Mason, Sally, Deepika Walpita, and Linda Mckay. "Melanotropin as a potential regulator of pigment pattern formation in embryonic skin." Pigment Cell Research 3.S2 (1990): 262-265. PubMed ID: 1329074

Sithigorngul, Paisarn, Antony OW Stretton, and Cynthia Cowden. "Neuropeptide diversity in Ascaris: an immunocytochemical study." Journal of Comparative Neurology 294.3 (1990): 362-376. PubMed ID: 2341616

Fernandez Jr, Philip Joseph. "The role of alpha-melanocyte stimulating hormone in environmentally induced color change." (1990).

Nahon, J. L., et al. "The Rat Melanin-Concentrating Hormone Messenger Ribonucleic Acid Encodes Multiple Putative Neuropeptides Coexpressed in the Dorsolateral Hypothalamus*." Endocrinology 125.4 (1989): 2056-2065. Tested in Rat, PubMed ID: 2477226

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Soinila, S., J. Lakshmanan, and T. Lahtinen. "Nerve growth factor-like activity in the rat pituitary intermediate lobe." Neuroscience research 6.2 (1988): 123-130. Tested in Rat, PubMed ID: 3217050

Bäck, N., et al. "Catecholamine-synthesizing enzymes in the rat pituitary." Histochemistry 86.5 (1987): 459-464. Tested in Rat, PubMed ID: 2884200

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Huettner, James E., and Robert W. Baughman. "Primary culture of identified neurons from the visual cortex of postnatal rats." The Journal of neuroscience 6.10 (1986): 3044-3060. Tested in Rat, PubMed ID: 3760948

Berrettini, Wade H., et al. "Pro-opiomelanocortin-related peptides in cerebrospinal fluid: a study of manic-depressive disorder." Psychiatry research 16.4 (1985): 287-302. PubMed ID: 3003774

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