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Alpha-MSH (Melanocyte Stimulating Hormone) Antibody

Alpha-MSH (Melanocyte Stimulating Hormone) Antibody
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Price: $250.00
Product ID : 20074
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Description

The ImmunoStar alpha melanocyte stimulating hormone antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pituitary using biotin/avidin-HRP techniques.

Recommended primary dilution is 1/4000-1/6000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 ug/mL of alpha-MSH.

Photo Description: IHC image of neurons staining for MSH in the rat hypothalamus (above) and rat pituitary with nickel preparation (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. 

MSH pituitary #20074

Host: Rabbit

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Reacts With: Pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Trout

Availability: In Stock

Alternate Names:  POMC; proopiomelanocortin 

Gene Symbol / ID, Accession #:  POMC,5443

 

Download Product Spec Sheet: Alpha-MSH (Melanocyte Stimulating Hormone) Antibody

 

Journal References:


Immunohistochemical localization of putative neurotransmitters within the feline nucleus tractus solitarii; B. Maley, R. Elde; Neuroscience,7(10):2469–2490, 1982, Tested in Cat, IFC, Cites ImmunoNuclear AKA ImmunoStar, PubMed ID: 6184644

Food deprivation-induced changes in body fat mobilization after neonatal monosodium glutamatetreatment; Claudia Leitner and Timothy J. Bartness; American Journal of Physiology, 294:R775-R783, 2008, Tested in Hamster, PubMed ID: 18094070


Borgquist, Amanda, et al. "Gonadal Steroids Differentially Modulate the Actions of Orphanin FQ/Nociceptin at A Physiologically Relevant Circuit Controlling Female Sexual Receptivity." Journal of neuroendocrinology 26.5 (2014): 329-340. Tested in Mouse, PubMed ID: 24617903

Iwasa, Masamitsu, Kazumi Kawabe, and Hreday N. Sapru. "Activation of melanocortin receptors in the intermediolateral cell column of the upper thoracic cord elicits tachycardia in the rat." American Journal of Physiology-Heart and Circulatory Physiology 305.6 (2013): H885-H893. Tested in Rat    PubMed ID: 23832700

Borgquist, Amanda, et al. "Estradiol Negatively Modulates the Pleiotropic Actions of Orphanin FQ/Nociceptin at Proopiomelanocortin Synapses." Neuroendocrinology 98.1 (2013): 60-72.

Kawabe, Tetsuya, Kazumi Kawabe, and Hreday N. Sapru. "Effect of barodenervation on cardiovascular responses elicited from the hypothalamic arcuate nucleus of the rat." PloS one 7.12 (2012): e53111.    Tested in    Rat, PubMed ID: 23300873

Kloukina, Vaia, et al. "G-protein-gated inwardly rectifying K+ channel 4 (GIRK4) immunoreactivity in chemically defined neurons of the hypothalamic arcuate nucleus that control body weight." Journal of chemical neuroanatomy 44.1 (2012): 14-23. PubMed ID: 22465809

Arcuate nucleus destruction does not block deprivation-induced increases in food foraging and hoarding food; Brain Research,1323:94-108 (2010), Tested in Hamster, PubMed ID: 20138163

Ryu, K‐Y., et al. "Loss of polyubiquitin gene Ubb leads to metabolic and sleep abnormalities in mice." Neuropathology and applied neurobiology 36.4 (2010): 285-299. Tested in Mouse, PubMed ID: 20002312

Farhang, Borzoo, et al. "The role of the NOP receptor in regulating food intake, meal pattern, and the excitability of proopiomelanocortin neurons." Neuropharmacology 59.3 (2010): 190-200. Tested in Mouse, PubMed ID: 20510254

Claudia Leitner, Timothy J. Bartness; Acute brown adipose tissue temperature response to cold in monosodium glutamate-treated Siberian hamsters; Brain Research,1292:3 8 – 5 1 (2009), Tested in    Hamster, PubMed ID: 19643091

Diaz, Shanna, et al. "Sex differences in the cannabinoid modulation of appetite, body temperature and neurotransmission at POMC synapses." Neuroendocrinology 89.4 (2009): 424-440. Tested in Guinea Pig and Mouse, PubMed ID: 19136814

Ramaswamy, S., R. B. Gibbs, and T. M. Plant. "Studies of the Localisation of Kisspeptin Within the Pituitary of the Rhesus Monkey (Macaca mulatta) and the Effect of Kisspeptin on the Release of Non‐Gonadotropic Pituitary Hormones." Journal of neuroendocrinology 21.10 (2009): 795-804. Tested in    Monkey, PubMed ID: 19686451

Kwon-Yul Ryu, Jacob C. Garza, Xin-Yun Lu, Gregory S. Barsh, and Ron R. Kopito; Hypothalamic neurodegeneration and adult-onset obesity in mice lacking the Ubb polyubiquitin gene; PNAS,105:4016-4021 (2008), Tested in Mouse, PubMed ID: 18299572

Jennie Ho, Jeremy M. Cox, and Edward J. Wagner; Cannabinoid-Induced Hyperphagia: Correlation With Inhibition Of Proopiomelanocortin Neurons?; Physiol Behav.,92(3):507-519 (2007), Tested in Guinea Pig, PubMed ID: 17532014

Koban, Michael, Wei Wei Le, and Gloria E. Hoffman. "Changes in hypothalamic corticotropin-releasing hormone, neuropeptide Y, and proopiomelanocortin gene expression during chronic rapid eye movement sleep deprivation of rats." Endocrinology 147.1 (2006): 421-431. Tested in    Rat, PubMed ID: 16210372

Nguyen, Que Huong, and Edward J. Wagner. "Estrogen differentially modulates the cannabinoid-induced presynaptic inhibition of amino acid neurotransmission in proopiomelanocortin neurons of the arcuate nucleus." Neuroendocrinology 84.2 (2006): 123-137. Tested in Guinea Pig, PubMed ID: 17106183

Bjorn Meister, Burcak Gomuc¸ Elisabet Suarez, Yuko Ishii, Katrin Durr and Linda Gillberg; Hypothalamic proopiomelanocortin (POMC) neurons; European Journal of Neuroscience,24:2731-2740 (2006), Tested in Rat, PubMed ID: 17156199

Meister, Björn, et al. "Hypothalamic proopiomelanocortin (POMC) neurons have a cholinergic phenotype." European Journal of Neuroscience 24.10 (2006): 2731-2740. Tested in Mouse and Rat, PubMed ID: 17586536

Allison Wanting Xu, Christopher B. Kaelin, Kiyoshi Takeda, Shizuo Akira, Michael W. Schwartz, and Gregory S. Barsh; PI3K integrates the action of insulin and leptin on hypothalamic neurons; The Journal of Clinical Investigation,115:951-958 (2005), Tested in Mouse, PubMed ID: 15761497

Zheng, Patterson, Phifer, Berthoud; Brainstem melanocortinergic modulation of meal size and identification of hypothalamic POMC projections; Am J Physiol Regul Integr Comp Physiol ,869:(1-29) (2004), Tested in Rat, PubMed ID: 15746303

Ismailov, Rashad, and George Galdava. "Interrelation of Immune, Antioxidant and Hormonal System Indices and their Influence on Melanogenesis in Patients with Skin Hyper Pigmentation." ANNALS OF BIOMEDICAL RESEARCH AND EDUCATION 4.4 (2004).

Reyes, Teresa M., and Paul E. Sawchenko. "Involvement of the arcuate nucleus of the hypothalamus in interleukin-1-induced anorexia." The Journal of neuroscience 22.12 (2002): 5091-5099. Tested in Rat, PubMed ID: 12077204

Przewlocki, Rysard, et al. "Opioid enhancement of calcium oscillations and burst events involving NMDA receptors and L-type calcium channels in cultured hippocampal neurons." The Journal of neuroscience 19.22 (1999): 9705-9715. Tested in Rat, PubMed ID: 10559380

MONROE, DAVID G., et al. "Agouti‐Related Maturation and Tissue Distribution of oc‐Melanocyte Stimulating Hormone in Wild‐Type (AwJ/AwJ) and Mutant (Ay/a, a/a) Mice." Pigment cell research 11.5 (1998): 310-313. Tested in Mouse, PubMed ID: 9877102

Clifton, V. L., et al. "Corticotropin-releasing hormone and proopiomelanocortin-derived peptides are present in human myometrium." Journal of Clinical Endocrinology & Metabolism 83.10 (1998): 3716-3721. Tested in Human, PubMed ID: 9768689

Sheng, Hui Z., et al. "Multistep control of pituitary organogenesis." Science 278.5344 (1997): 1809-1812. PubMed ID: 9388186

Eaker, Ervin Y. "Neurofilament and intermediate filament immunoreactivity in human intestinal myenteric neurons." Digestive diseases and sciences 42.9 (1997): 1926-1932. Tested in Human, PubMed ID: 9331157

Yáñez, J., et al. "The neuronal system of the saccus vasculosus of trout (Salmo trutta fario and Oncorhynchus mykiss): an immunocytochemical and nerve tracing study." Cell and tissue research 288.3 (1997): 497-507. Tested in Trout, PubMed ID: 9134862

Ogawa, Kazushige, Eiko Suziku, and Kazuyuki Taniguchi. "Immunohistochemical studies on the development of the hypothalamo‐hyophysial system in Xenopus laevis." The Anatomical Record 241.2 (1995): 244-254. Tested in Frog (Xenopus Laevis), PubMed ID: 7710140

Evans, VINCENT R., et al. "Alpha-melanocyte-stimulating hormone and N-acetyl-beta-endorphin immunoreactivities are localized in the human pituitary but are not restricted to the zona intermedia." Endocrinology 134.1 (1994): 97-106. Tested in Human, PubMed ID: 8275975

Lloyd, Ricardo V., et al. "Corticotroph (Basophil) invasion of the pars nervosa in the human pituitary: Localization of proopiomelanocortin peptides, galanin and peptidylglycine α-amidating monooxygenase-like immunoreactivities." Endocrine Pathology 4.2 (1993): 86-94.

Quinn, J., F. P. Eckenstein, and R. W. Baughman. "Novel antigenic determinant expressed in neurons of the dorsolateral hypothalamus in rat and human." Journal of neuroscience research 31.4 (1992): 715-723. Tested in Rat and Human, PubMed ID: 1374480

Madden, John, et al. "Isolation and characterization of opioid peptides from rabbit cerebellum." Journal of neurochemistry 56.6 (1991): 1914-1920. Tested in Rabbit, PubMed ID: 2027006

Carnahan, J. F., and P. H. Patterson. "The generation of monoclonal antibodies that bind preferentially to adrenal chromaffin cells and the cells of embryonic sympathetic ganglia." The Journal of neuroscience 11.11 (1991): 3493-3506.    PubMed ID: 1719162

O'Shea, K. Sue, L-HJ Liu, and V. M. Dixita. "Thrombospondin and a 140 kd fragment promote adhesion and neurite outgrowth from embryonic central and peripheral neurons and from PC12 cells." Neuron 7.2 (1991): 231-237. PubMed ID: 1873028

Ryder, Elizabeth F., Evan Y. Snyder, and Constance L. Cepko. "Establishment and characterization of multipotent neural cell lines using retrovirus vector‐mediated oncogene transfer." Journal of neurobiology 21.2 (1990): 356-375. PubMed ID: 2307979

Frost‐Mason, Sally, Deepika Walpita, and Linda Mckay. "Melanotropin as a potential regulator of pigment pattern formation in embryonic skin." Pigment Cell Research 3.S2 (1990): 262-265. PubMed ID: 1329074

Sithigorngul, Paisarn, Antony OW Stretton, and Cynthia Cowden. "Neuropeptide diversity in Ascaris: an immunocytochemical study." Journal of Comparative Neurology 294.3 (1990): 362-376. PubMed ID: 2341616

Fernandez Jr, Philip Joseph. "The role of alpha-melanocyte stimulating hormone in environmentally induced color change." (1990).

Nahon, J. L., et al. "The Rat Melanin-Concentrating Hormone Messenger Ribonucleic Acid Encodes Multiple Putative Neuropeptides Coexpressed in the Dorsolateral Hypothalamus*." Endocrinology 125.4 (1989): 2056-2065. Tested in Rat, PubMed ID: 2477226

Weihe, E., et al. "Multiplicity of opioidergic pathways related to cardiovascular innervation: differential contribution of all three opioid precursors." Opioid Peptides and Blood Pressure Control. Springer Berlin Heidelberg, 1988. 27-49.

Soinila, S., J. Lakshmanan, and T. Lahtinen. "Nerve growth factor-like activity in the rat pituitary intermediate lobe." Neuroscience research 6.2 (1988): 123-130. Tested in Rat, PubMed ID: 3217050

Bäck, N., et al. "Catecholamine-synthesizing enzymes in the rat pituitary." Histochemistry 86.5 (1987): 459-464. Tested in Rat, PubMed ID: 2884200

Bixby, John L., and Louis F. Reichardt. "Effects of antibodies to neural cell adhesion molecule (N-CAM) on the differentiation of neuromuscular contacts between ciliary ganglion neurons and myotubesin vitro." Developmental biology 119.2 (1987): 363-372. PubMed ID: 3803709

Vinnitsky, V. B., and G. V. Glinsky. "Role of the Binding of Neuropeptides to Blood Plasma Proteins in the Control of Their Blood‐Brain Barrier Passage." Annals of the New York Academy of Sciences 496.1 (1987): 278-291. PubMed ID: 3474972

Levine, Joel M., and Patricia Flynn. "Cell surface changes accompanying the neural differentiation of an embryonal carcinoma cell line." The Journal of neuroscience 6.11 (1986): 3374-3384. PubMed ID: 3021929

Gabrilove, J. L., P. J. Anderson, and N. S. Halmi. "PITUITARY PRO‐OPIOMELANOCORTIN‐CELL CARCINOMA OCCURRING IN CONJUNCTION WITH A GLIOBLASTOMA IN A PATIENT WITH CUSHING'S DISEASE AND SUBSEQUENT NELSON'S SYNDROME." Clinical endocrinology 25.2 (1986): 117-126. PubMed ID: 3024876

Huettner, James E., and Robert W. Baughman. "Primary culture of identified neurons from the visual cortex of postnatal rats." The Journal of neuroscience 6.10 (1986): 3044-3060. Tested in Rat, PubMed ID: 3760948

Berrettini, Wade H., et al. "Pro-opiomelanocortin-related peptides in cerebrospinal fluid: a study of manic-depressive disorder." Psychiatry research 16.4 (1985): 287-302. PubMed ID: 3003774

Bäck, Nils, and Leena Rechardt. "The effect of reserpine on the pars intermedia of the rat pituitary." Cell and tissue research 241.1 (1985): 1-8. Tested in Rat, PubMed ID: 3161619

Dräger, Ursula C., D. Louise Edwards, and Jochen Kleinschmidt. "Neurofilaments contain alpha-melanocyte-stimulating hormone (alpha-MSH)-like immunoreactivity." Proceedings of the National Academy of Sciences 80.20 (1983): 6408-6412. Tested in Mouse, PubMed ID: 6194532

Micevych, Paul E., and Robert P. Elde. "Neurons containing α-melanocyte stimulating hormone and β-endorphin immunoreactivity in the cat hypothalamus." Peptides 3.4 (1982): 655-662. Tested in Cat, PubMed ID: 6291010

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