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5-HT (Serotonin) Transporter Antibody

5-HT (Serotonin) Transporter Antibody
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Price: $375.00
Product ID : 24330
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Description

The ImmunoStar serotonin (5HT) transporter was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat raphe nuclei, hypothalamus, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions for these methods are 1/800 - 1/1,000 in PBS/0.3% Triton X-100 - Cy3 and 1/10,000 - 1/15,000 in PBS/0.3% Triton X-100 - Biotin/avidin-HRP.

The above image is of Serotonin Transporter positive fiber staining in rat cortex, cryostat sections, Cy3 (Jackson) detection.

By Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, the antibody specifically labels a single band. The western information is included as specificity information only.  Due to the difficulty in running westerns with this antibody, ImmunoStar does not warrant the western blot application.

Immunolabeling is completely abolished by pre-adsorption with synthetic rat 5HT transporter (602-622).

 

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Availability: In Stock

Reacts With: Human, Marine, Monkey, Mouse, Rat, Sparrow

Alternate Names:  5HTT; HTT; Sodium-dependent serotonin transporter; Solute carrier family 6 member 4; Slc6a4; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4

Gene Symbol / ID, Accession #:  Slc6a4,25553

 

Download Product Spec Sheet5-HT (Serotonin) Transporter Antibody 

 

Journal References:


Cortex-restricted disruption of NMDAR1 impairs neuronal patterns in the barrel cortex.; Iwasato T, Datwani A, Wolf AM, Nishiyama H, et al; Nature,406(17):726-731, 2000, Tested in Mouse, Cites Diasorin AKA ImmunoStar, PubMed ID: 10963597

BDNF Promotes the Regenerative Sprouting, But Not Survival, of Injured Serotonergic Axons in the Adult Rat Brain; Mamounas LA, Altar CA, Blue ME; The Journal of Neuroscience,20(2):771-782, 2000, Tested in Rat, Cites Incstar AKA ImmunoStar, PubMed ID: 10632606

Immunocytochemical Detection Of The Serotonin Transporter In Rat Brain; C. Sur, H. Betz And P. Schloss; Neuroscience,73:217-231, 1996, Tested in Rat, IFC, Cites Incstar AKA ImmunoStar, PubMed ID: 8783244

Function, expression, and characterization of the serotonin transporter in the native human intestine; Ravinder K. Gill, Nitika Pant, Seema Saksena, Amika Singla, Talat M. Nazir, Lisa Vohwinkel, Jerrold R. Turner, Jay Goldstein, Waddah A. Alrefai, and Pradeep K. Dudeja; Am J Physiol Gastrointest Liver Physiol,294:G254-G262, 2008, Tested in Human , Western Blot, IFC, PubMed ID: 17991706

The pre-nervous serotonergic system of developing sea urchin embryos and larvae: pharmacologic and immunocytochemical evidence; Gennady A. Buznikov,  Robert E. Lyudmila A. Nikitina, Vladimir V. , and Jean M. Lauder; Neurochemical Research,30:825–837, 2005, Tested in Marine, PubMed ID: 16187217

Abnormal thalamocortical pathfinding and terminal arbors lead to enlarged barrels in neonatal GAP-43 heterozygous mice; McIlvain VA, Robertson DR, Maimone MM, McCasland JS.; J Comp Neurol., 462(2):252-64, 2003, Tested in Mouse, PubMed ID: 12794747

Evidence that serotonin reuptake modulators increase the density of serotonin innervation in the forebrain; Zhou, Huang, Kecojevic, Welsh, Koliatsos; Journal of Neurochemistry,396(2):396-?, 2006, Tested in Rat, PubMed ID: 16300628

Anatomical and functional characterization of neuropil in the gracile fasciculus; Ramer MS.; J Comp Neurol.,510(3):283-96, 2008, Tested in Rat, PubMed ID: 18634004

5-HT Precursor Loading But Not 5-HT Receptor Agonists Increases Motor Function After Spinal Cord Contusion in Adults Rats; Y. Hayashi, S. Jacob-Vadakot, E.A. Dugan, S. McBride, R. Olexa, K. Simansky, M. Murray, and J.S. Shumsky; Exp Neurol.,221(1):68–78, 2009, Tested in Rat, PubMed ID: 19840787

The Interface of Oxytocin-Labeled Cells and Serotonin Transporter-Containing Fibers in the Primate Hypothalamus: A Substrate for SSRIs Therapeutic Effects; Ana BF Emiliano, Thomaz Cruz, Valerie Pannoni and Julie L Fudge; Neuropsychopharmacology,32:977-988, 2007, Tested in Monkey, PubMed ID: 17035935

Barrel Map Development Relies on Protein Kinase A Regulatory Subunit IIbeta-Mediated cAMP Signaling ; Inan, Lu, Albright, She, Crair; The Journal of Neuroscience,26(16):4338-4349, 2006, Tested in Mouse, PubMed ID: 16624954

Increased Thalamocortical Synaptic Response and Decreased Layer IV Innervation in GAP-43 Knockout Mice; Albright MJ, Weston MC, Inan M, Rosenmund C, Crair MC.; J Neurophysiol,98:1610-1625, 2007, Tested in Mouse, PubMed ID: 17581849

Experimental evidence that the serotonin transporter mediates serotonin accumulation in LSO neurons of the postnatal mouse; Ann M. Thompsona, Glenn C. Thompson; Brain Research,1253:60-68, 2009, Tested in Mouse, PubMed ID: 19070605

Increased hippocampal CA1 density of serotonergic terminals in a triple transgenic mouse model of Alzheimer’s disease: an ultrastructural study; H N Noristani, R S Meadows, M Olabarria, A Verkhratsky, J J Rodríguez; Cell Death and Disease,2:e210, 2011, Tested in Mouse, PubMed ID: 21918544

Plasticity of serotonergic innervation of the inferior colliculus in mice following acoustic trauma; Melissa A. Papesha and Laura M. Hurley; Hear Res.,283(1-2):89-97, 2012, Tested in Mouse, PubMed ID: 22101024

Afferent and efferent connections of the rat retrotrapezoid nucleus; Rosin DL, Chang DA, Guyenet PG.; J Comp Neurol.,122(6):745-752, 1990, Tested in Rat, PubMed ID: 16958085

A soluble Nogo receptor differentially affects plasticity of spinally projecting axons ; MacDermid VE, McPhail LT, Tsang B, Rosenthal A , Davies A, Ramer MS; European Journal of Neuroscience,:, 2004, Tested in Rat, PubMed ID: 15548200

"Skin-Derived Precursors Generate Myelinating Schwann Cells That Promote Remyelination and Functional Recovery after Contusion Spinal Cord Injury; Jeff Biernaskie, Joseph S. Sparling, Jie Liu, Casey P. Shannon, Jason R. Plemel,  Yuanyun Xie,
Freda D. Miller, and Wolfram Tetzlaff; Journal of Neuroscience,27(36):9545–9559, 2007, Tested in Rat, PubMed ID: 17804616"

The unusual response of serotonergic neurons after CNS injury: lack of axonal dieback and enhanced sprouting within the inhibitory environment of the glial scar; Alicia L. Hawthorne, Hongmei Hu, Bornali Kundu, Michael P. Steinmetz, Christi J. Wylie, Evan S. Deneris, Jerry Silver; J Neurosci,31(15):1-24, 2011, Tested in Rat, PubMed ID: 21490201

Intermittent Fasting Improves Functional Recovery after Rat Thoracic Contusion Spinal Cord Injury; Mi-ae Jeong, Ward Plunet, Femke Streijger, Jae H.T. Lee, Jason R. Plemel, Sophia Park, Clarrie K. Lam, Jie Liu, Wolfram Tetzlaff; J Neurotrauma,28(3):479–492, 2011, Tested in Rat, PubMed ID: 21219083

Estradiol-Dependent Modulation of Serotonergic Markers in Auditory Areas of a Seasonally Breeding Songbird; Matragrano LL, Sanford SE, Salvante KG, Beaulieu M, Sockman KW, Maney DL.; Behavioral Neuroscience,126(1):110-122, 2012, Tested in Sparrow, PubMed ID: 21942431

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