5-HT (Serotonin) Transporter Antibody

5-HT (Serotonin) Transporter Antibody
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Price: $375.00
Product ID : 24330
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The ImmunoStar serotonin (5HT) transporter was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat raphe nuclei, hypothalamus, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/10,000 - 1/15,000 in PBS/0.3% Triton X-100 - Biotin/avidin-HRP.

By Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, the antibody specifically labels a single band. The western information is included as specificity information only.  Due to the difficulty in running westerns with this antibody, ImmunoStar does not warrant the western blot application.

Immunolabeling is completely abolished by pre-adsorption with synthetic rat 5HT transporter (602-622).

Photo Description: IHC image of neurons staining for the 5-HT transporter in the raphe nucleus of the rat brainstem (above). The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Florescent secondary (below)

5Ht Transporter #14330

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Availability: In Stock

Reacts With: Human, Marine, Mouse, Rat, Sparrow

Alternate Names:  5HTT; HTT; Sodium-dependent serotonin transporter; Solute carrier family 6 member 4; Slc6a4; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4

Gene Symbol / ID, Accession #:  Slc6a4,25553


Download Product Spec Sheet5-HT (Serotonin) Transporter Antibody 


Journal References:

BDNF Promotes the Regenerative Sprouting, But Not Survival, of Injured Serotonergic Axons in the Adult Rat Brain; Mamounas LA, Altar CA, Blue ME; The Journal of Neuroscience,20(2):771-782, 2000, Tested in Rat, Cites Incstar AKA ImmunoStar, PubMed ID: 10632606

Abnormal thalamocortical pathfinding and terminal arbors lead to enlarged barrels in neonatal GAP-43 heterozygous mice; McIlvain VA, Robertson DR, Maimone MM, McCasland JS.; J Comp Neurol., 462(2):252-64, 2003, Tested in Mouse, PubMed ID: 12794747

The Interface of Oxytocin-Labeled Cells and Serotonin Transporter-Containing Fibers in the Primate Hypothalamus: A Substrate for SSRIs Therapeutic Effects; Ana BF Emiliano, Thomaz Cruz, Valerie Pannoni and Julie L Fudge; Neuropsychopharmacology,32:977-988, 2007, Tested in Monkey, PubMed ID: 17035935

A soluble Nogo receptor differentially affects plasticity of spinally projecting axons ; MacDermid VE, McPhail LT, Tsang B, Rosenthal A , Davies A, Ramer MS; European Journal of Neuroscience,:, 2004, Tested in Rat, PubMed ID: 15548200

Bruzos-Cidón, C., et al. "Altered neuronal activity and differential sensitivity to acute antidepressants of locus coeruleus and dorsal raphe nucleus in Wistar Kyoto rats: A comparative study with Sprague Dawley and Wistar rats." European Neuropsychopharmacology (2014). Tested in Mouse, PubMed ID: 24582527

Arrant, Andrew E., Hikma Jemal, and Cynthia M. Kuhn. "Adolescent male rats are less sensitive than adults to the anxiogenic and serotonin-releasing effects of fenfluramine." Neuropharmacology 65 (2013): 213-222. Tested in Rat, PubMed ID: 23103347

Van Dine, Sarah E., et al. "Axonal anatomy of molecular layer heterotopia of the cerebellar vermis." Journal of chemical neuroanatomy 47 (2013): 90-95. PubMed ID: 23261868

Seimandi, Mathieu, et al. "Calcineurin Interacts with the Serotonin Transporter C-Terminus to Modulate Its Plasma Membrane Expression and Serotonin Uptake." The Journal of Neuroscience 33.41 (2013): 16189-16199. Tested in Mouse, PubMed ID: 24107951

Van Dine, Sarah E., et al. "Cellular and Axonal Diversity in Molecular Layer Heterotopia of the Rat Cerebellar Vermis." BioMed research international 2013 (2013). Tested in Rat, PubMed ID: 24191251

Hong, Diana. "Immunohistochemical quantification of serotonin and serotonin transporters in the lumbar region of the mouse spinal cord after spinal cord injury." (2013). Tested in Rat.

Aristieta, A., et al. "Modulation of the subthalamic nucleus activity by serotonergic agents and fluoxetine administration." Psychopharmacology (2013): 1-12. Tested in Rat, PubMed ID: 24271033

Zhang, Junlin, et al. "Neonatal citalopram exposure decreases serotonergic fiber density in the olfactory bulb of male but not female adult rats." Frontiers in cellular neuroscience 7 (2013). Tested in Rat, PubMed ID: 23675318

Linley, Stephanie B., Walter B. Hoover, and Robert P. Vertes. "Pattern of distribution of serotonergic fibers to the orbitomedial and insular cortex in the rat." Journal of chemical neuroanatomy 48 (2013): 29-45.    Tested in Rat, PubMed ID: 23337940

Matragrano LL, Sanford SE, Salvante KG, Beaulieu M, Sockman KW, Maney DL.; Estradiol-Dependent Modulation of Serotonergic Markers in Auditory Areas of a Seasonally Breeding Songbird; Behavioral Neuroscience,126(1):110-122 (2012), Tested in Sparrow, PubMed ID: 21942431

Assimacopoulos, Stavroula, et al. "Fibroblast growth factor 8 organizes the neocortical area map and regulates sensory map topography." The Journal of Neuroscience 32.21 (2012): 7191-7201. Tested in Mouse, PubMed ID: 22623663

Li, Kairong, Jiaqing Zhang, and James YH Li. "Gbx2 plays an essential but transient role in the formation of thalamic nuclei." PloS one 7.10 (2012): e47111. Tested in Mouse, PubMed ID: 23056596

Papesh, Melissa A., and Laura M. Hurley. "Plasticity of serotonergic innervation of the inferior colliculus in mice following acoustic trauma." Hearing research 283.1 (2012): 89-97. Tested in Mouse, PubMed ID: 22101024

Husch, Andreas, et al. "Spinal cord injury induces serotonin supersensitivity without increasing intrinsic excitability of mouse V2a interneurons." The Journal of Neuroscience 32.38 (2012): 13145-13154. Tested in Mouse, PubMed ID: 22993431

André, Pascal, et al. "Transport of biogenic amine neurotransmitters at the mouse blood–retina and blood–brain barriers by uptake1 and uptake2." Journal of Cerebral Blood Flow & Metabolism 32.11 (2012): 1989-2001. Tested in Mouse, PubMed ID: 22850405

Bonnin, Alexandre, et al. "A transient placental source of serotonin for the fetal forebrain." Nature 472.7343 (2011): 347-350. Tested in Human and Mouse, PubMed ID: 21512572

Bertrand, R. L., et al. "A Western diet increases serotonin availability in rat small intestine." Endocrinology 152.1 (2011): 36-47. Tested in Rat, PubMed ID: 21068163

H N Noristani, R S Meadows, M Olabarria, A Verkhratsky, J J Rodríguez; Increased hippocampal CA1 density of serotonergic terminals in a triple transgenic mouse model of Alzheimer’s disease: an ultrastructural study; Cell Death and Disease,2:e210 (2011), Tested in Mouse, PubMed ID: 21918544

Mi-ae Jeong, Ward Plunet, Femke Streijger, Jae H.T. Lee, Jason R. Plemel, Sophia Park, Clarrie K. Lam, Jie Liu, Wolfram Tetzlaff; Intermittent Fasting Improves Functional Recovery after Rat Thoracic Contusion Spinal Cord Injury; J Neurotrauma,28(3):479–492 (2011), Tested in Rat, PubMed ID: 21219083

RodrÍGuez, José J., et al. "Serotonergic projections and serotonin receptor expression in the reticular nucleus of the thalamus in the rat." Synapse 65.9 (2011): 919-928. Tested in Rat, PubMed ID: 21308802

Alicia L. Hawthorne, Hongmei Hu, Bornali Kundu, Michael P. Steinmetz, Christi J. Wylie, Evan S. Deneris, Jerry Silver; The unusual response of serotonergic neurons after CNS injury: lack of axonal dieback and enhanced sprouting within the inhibitory environment of the glial scar; J Neurosci,31(15):1-24 (2011), Tested in Rat, PubMed ID: 21490201

Sanno, Hitomi, et al. "Control of Postnatal Apoptosis in the Neocortex byRhoA-Subfamily GTPases Determines Neuronal Density." The Journal of Neuroscience 30.12 (2010): 4221-4231. Tested in Mouse, PubMed ID: 20335457

Cragg, J. J., A. L. Scott, and M. S. Ramer. "Depletion of spinal 5-HT accelerates mechanosensory recovery in the deafferented rat spinal cord." Experimental neurology 222.2 (2010): 277-284. Tested in Rat, PubMed ID: 20079735

Scott, Angela LM, and Matt S. Ramer. "Differential regulation of dendritic plasticity by neurotrophins following deafferentation of the adult spinal cord is independent of p75(NTR)." Brain research 1323 (2010): 48-58. PubMed ID: 20144886

Peters, Christopher M., et al. "Lack of analgesic efficacy of spinal ondansetron on thermal and mechanical hypersensitivity following spinal nerve ligation in the rat." Brain research 1352 (2010): 83-93. Tested in Rat, PubMed ID: 20637741

Weaver, Kristin J., et al. "Neonatal exposure to citalopram selectively alters the expression of the serotonin transporter in the hippocampus: dose‐dependent effects." The Anatomical Record 293.11 (2010): 1920-1932. Tested in Rat, PubMed ID: 20830689

Y. Hayashi, S. Jacob-Vadakot, E.A. Dugan, S. McBride, R. Olexa, K. Simansky, M. Murray, and J.S. Shumsky; 5-HT Precursor Loading But Not 5-HT Receptor Agonists Increases Motor Function After Spinal Cord Contusion in Adults Rats; Exp Neurol.,221(1):68–78 (2009). Tested in Rat, PubMed ID: 19840787

Dudok, Jacobus J., et al. "Chronic activation of the 5-HT 2 receptor reduces 5-HT neurite density as studied in organotypic slice cultures." Brain research 1302 (2009): 1-9. PubMed ID: 19728996

Nayyar, Tultul, et al. "Cortical serotonin and norepinephrine denervation in parkinsonism: preferential loss of the beaded serotonin innervation." European Journal of Neuroscience 30.2 (2009): 207-216. Tested in Mouse, PubMed ID: 19659923

Esmaili, Ali, et al. "Enteropathogenic Escherichia coli Infection Inhibits Intestinal Serotonin Transporter Function and Expression." Gastroenterology 137.6 (2009): 2074-2083. Tested in Mouse, PubMed ID: 19747920

Ann M. Thompsona, Glenn C. Thompson; Experimental evidence that the serotonin transporter mediates serotonin accumulation in LSO neurons of the postnatal mouse; Brain Research,1253:60-68 (2009), Tested in Mouse, PubMed ID: 19070605

Kalous, Adrianna, Peregrine B. Osborne, and Janet R. Keast. "Spinal cord compression injury in adult rats initiates changes in dorsal horn remodeling that may correlate with development of neuropathic pain." Journal of Comparative Neurology 513.6 (2009): 668-684. Tested in Rat, PubMed ID: 19235905

Ramer MS.; Anatomical and functional characterization of neuropil in the gracile fasciculus; J Comp Neurol.,510(3):283-96 (2008), Tested in Rat, PubMed ID: 18634004

Ravinder K. Gill, Nitika Pant, Seema Saksena, Amika Singla, Talat M. Nazir, Lisa Vohwinkel, Jerrold R. Turner, Jay Goldstein, Waddah A. Alrefai, and Pradeep K. Dudeja; Function, expression, and characterization of the serotonin transporter in the native human intestine; Am J Physiol Gastrointest Liver Physiol,294:G254-G262 (2008), Tested in    Human, PubMed ID: 17991706

Bretzner, Frederic, et al. "Undesired effects of a combinatorial treatment for spinal cord injury–transplantation of olfactory ensheathing cells and BDNF infusion to the red nucleus." European Journal of Neuroscience 28.9 (2008): 1795-1807. Tested in Mouse, PubMed ID: 18973595

Kalous, Adrianna, Peregrine B. Osborne, and Janet R. Keast. "Acute and chronic changes in dorsal horn innervation by primary afferents and descending supraspinal pathways after spinal cord injury." Journal of Comparative Neurology 504.3 (2007): 238-253. Tested in Rat, PubMed ID: 17640046

Albright MJ, Weston MC, Inan M, Rosenmund C, Crair MC.; Increased Thalamocortical Synaptic Response and Decreased Layer IV Innervation in GAP-43 Knockout Mice; J Neurophysiol,98:1610-1625 (2007). Tested in Mouse, PubMed ID: 17581849

Jeff Biernaskie, Joseph S. Sparling, Jie Liu, Casey P. Shannon, Jason R. Plemel,  Yuanyun Xie,
Freda D. Miller, and Wolfram Tetzlaff; Skin-Derived Precursors Generate Myelinating Schwann Cells That Promote Remyelination and Functional Recovery after Contusion Spinal Cord Injury; Journal of Neuroscience,27(36):9545–9559 (2007). Tested in Rat, PubMed ID: 17804616

Inan, Lu, Albright, She, Crair; Barrel Map Development Relies on Protein Kinase A Regulatory Subunit IIbeta-Mediated cAMP Signaling; The Journal of Neuroscience,26(16):4338-4349 (2006). Tested in Mouse, PubMed ID: 16624954

Zhou, Huang, Kecojevic, Welsh, Koliatsos; Evidence that serotonin reuptake modulators increase the density of serotonin innervation in the forebrain; Journal of Neurochemistry,396(2):396-? (2006). Tested in Rat, PubMed ID: 16300628

Xie, Tao, et al. "Loss of serotonin transporter protein after MDMA and other ring-substituted amphetamines." Neuropsychopharmacology 31.12 (2006): 2639-2651. Tested in Rat, PubMed ID: 16452989

Janušonis, Skirmantas, et al. "Ontogeny of brain and blood serotonin levels in 5‐HT1A receptor knockout mice: potential relevance to the neurobiology of autism." Journal of neurochemistry 99.3 (2006): 1019-1031. Tested in Mouse, PubMed ID: 16981893

Hayat, Shaista, Robert J. Williams, and Marcus Rattray. "Serotonin transporter expression is not sufficient to confer cytotoxicity to 3, 4-methylenedioxymethamphetamine (MDMA) in vitro." Journal of Psychopharmacology 20.2 (2006): 257-263.    Tested in Mouse and Rat, PubMed ID: 16510483

Lee, Li‐Jen, et al. "Exuberant thalamocortical axon arborization in cortex‐specific NMDAR1 knockout mice." Journal of Comparative Neurology 485.4 (2005): 280-292. Tested in Mouse, PubMed ID: 15803506

Shimogori, Tomomi, and Elizabeth A. Grove. "Fibroblast growth factor 8 regulates neocortical guidance of area-specific thalamic innervation." The Journal of neuroscience 25.28 (2005): 6550-6560. Tested in Mouse, PubMed ID: 16014716

De Silva, D. J., et al. "Rat brain serotonin neurones that express neuronal nitric oxide synthase have increased sensitivity to the substituted amphetamine serotonin toxins 3, 4-methylenedioxymethamphetamine andp-chloroamphetamine." Neuroscience 134.4 (2005): 1363-1375. Tested in    Rat, PubMed ID: 16054768

Gennady A. Buznikov,  Robert E. Lyudmila A. Nikitina, Vladimir V. , and Jean M. Lauder; The pre-nervous serotonergic system of developing sea urchin embryos and larvae: pharmacologic and immunocytochemical evidence; Neurochemical Research,30:825–837 (2005). Tested in Marine, PubMed ID: 16187217

Rumajogee, Prakasham, et al. "Adaption of the serotoninergic neuronal phenotype in the absence of 5‐HT autoreceptors or the 5‐HT transporter: involvement of BDNF and cAMP." European Journal of Neuroscience 19.4 (2004): 937-944. Tested in Mouse, PubMed ID: 15009141

Koldzic-Zivanovic, Nina, et al. "Intracellular signaling involved in estrogen regulation of serotonin reuptake." Molecular and cellular endocrinology 226.1 (2004): 33-42. Tested in Rat, PubMed ID: 15489003

Meyer, Jerrold S., et al. "Neurotoxic effects of MDMA (“ecstasy”) administration to neonatal rats." International journal of developmental neuroscience 22.5 (2004): 261-271. Tested in Rat, PubMed ID: 15380826

Donovan, Stacy L., et al. "GAP-43 is critical for normal development of the serotonergic innervation in forebrain." The Journal of neuroscience 22.9 (2002): 3543-3552. Tested in Mouse, PubMed ID: 11978831

Datwani, Akash, et al. "Lesion-induced thalamocortical axonal plasticity in the S1 cortex is independent of NMDA receptor function in excitatory cortical neurons." The Journal of neuroscience 22.21 (2002): 9171-9175. Tested in Mouse, PubMed ID: 12417641

Rumajogee, Prakasham, et al. "Up‐regulation of the neuronal serotoninergic phenotype in vitro: BDNF and cAMP share Trk B‐dependent mechanisms." Journal of neurochemistry 83.6 (2002): 1525-1528. Tested in Rat, PubMed ID: 12472905

Kubota, Nobuo, et al. "Regulation of serotonin transporter gene expression in human glial cells by growth factors." European journal of pharmacology 417.1 (2001): 69-76. Tested in    Human, PubMed ID: 11301061

Mamounas, Laura A., et al. "BDNF promotes the regenerative sprouting, but not survival, of injured serotonergic axons in the adult rat brain." The Journal of Neuroscience 20.2 (2000): 771-782. Tested in Rat, PubMed ID: 10632606

Iwasato, Takuji, et al. "Cortex-restricted disruption of NMDAR1 impairs neuronal patterns in the barrel cortex." Nature 406.6797 (2000): 726-731. Tested in Mouse, PubMed ID: 10963597

Brown, Pierre, and Mark E. Molliver. "Dual serotonin (5-HT) projections to the nucleus accumbens core and shell: relation of the 5-HT transporter to amphetamine-induced neurotoxicity." The Journal of Neuroscience 20.5 (2000): 1952-1963. Tested in Rat, PubMed ID: 10684896

C. Sur, H. Betz And P. Schloss; Immunocytochemical Detection Of The Serotonin Transporter In Rat Brain    Neuroscience; 73:217-231 (1996); Tested in Rat, PubMed ID: 8783244

Sur, Cyrille, Heinrich Betz, and Patrick Schloss. "Localization of the serotonin transporter in rat spinal cord." European Journal of Neuroscience 8.12 (1996): 2753-2757. Tested in Rat, PubMed ID: 8996825

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