5-HT (Serotonin) Transporter Antibody

The ImmunoStar serotonin (5HT) transporter was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat raphe nuclei, hypothalamus, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions for these methods are 1/800 - 1/1,000 in PBS/0.3% Triton X-100 - Cy3 and 1/10,000 - 1/15,000 in PBS/0.3% Triton X-100 - Biotin/avidin-HRP.

By Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, the antibody specifically labels a single band. Immunolabeling is completely abolished by pre-adsorption with synthetic rat 5HT transporter (602-622).

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Availability: In Stock

Reacts With: Human, Monkey, Mouse, Rat 

Predicted to React With: Bovine, Frog, Guinea Pig, Monkey, Sheep Bird

Download Product Spec Sheet:  5-HT (Serotonin) Transporter Antibody 

Journal References:

Skin-Derived Precursors Generate Myelinating Schwann Cells That Promote Remyelination and Functional Recovery after Contusion Spinal Cord Injury, Jeff Biernaskie, Joseph S. Sparling, Jie Liu, Casey P. Shannon, Jason R. Plemel,  Yuanyun Xie, Freda D. Miller, and Wolfram Tetzlaff, Journal of Neuroscience, 27(36), 9545–9559, 2007, rat

Abnormal thalamocortical pathfinding and terminal arbors lead to enlarged barrels in neonatal GAP-43 heterozygous mice., McIlvain VA, Robertson DR, Maimone MM, McCasland JS, J Comp Neurol , 462(2), 252-264, 2003.

Barrel Map Development Relies on Protein Kinase A Regulatory Subunit IIbeta-Mediated cAMP Signaling , Inan, Lu, Albright, She, Crair, The Journal of Neuroscience, 26(16), 4338-4349, 2006.

BDNF Promotes the Regenerative Sprouting, But Not Survival, of Injured Serotonergic Axons in the Adult Rat Brain, Mamounas LA, Altar CA, Blue ME, The Journal of Neuroscience, 20(2), 771-782, 2000.

Cortex-restricted disruption of NMDAR1 impairs neuronal patterns in the barrel cortex., Iwasato T, Datwani A, Wolf AM, Nishiyama H, et al, Nature, 406(17), 726-731, 2000. 

A soluble Nogo receptor differentially affects plasticity of spinally projecting axons , MacDermid VE, McPhail LT, Tsang B, Rosenthal A,  Davies A, Ramer MS, European Journal of Neuroscience.

The Interface of Oxytocin-Labeled Cells and Serotonin Transporter-Containing Fibers in the Primate Hypothalamus: A Substrate for SSRIs Therapeutic Effects?, Ana BF Emiliano1, Thomaz Cruz1, Valerie Pannoni2 and Julie L Fudge, Neuropsychopharmacology, 32, 977-988, 2007, Tested in Monkey.