5-HT (Serotonin) Rabbit Antibody
; "5-HT (Serotonin) Rabbit Antibody")
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Price: $275.00
Product ID : 20080
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Description
The ImmunoStar serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, raphe nuclei and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000-1/2,000 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/20,000-1/40,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 25 ug of serotonin/BSA.
Cross reactivity of Serotonin antisera was examined. With 5µg, 10µg and 25µg amounts the following substances did not react with Serotonin antisera diluted 1/20,000 using the Bn-SA/HRP labeling method: 5-hydroxytryptophan, 5-hydroxyindole -3- acetic acid, and dopamine.
Host: Rabbit
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Annelida, Arthropod, Cat , Crab, Fly, Frog, Grasshopper, Hamster, Hermit Crab, Human, Lamprey, Lobster, Mollusc, Monkey, Moth, Mouse, Platypusechidna, Mouse, Rat, Salamander, Zebrafish
Availability: In Stock
Download Product Spec Sheet: 5-HT (Serotonin) Rabbit Antibody
Download full library of journal references: 5-HT (Serotonin) Rabbit References
Recent Journal References:
Arrest of 5HT neuron differentiation delays respiratory maturation and impairs neonatal homeostatic responses to environmental challenges. Jeffery T. Erickson, Geoffrey Shafer, Michael D. Rossetti, Christopher G. Wilson, Evan S. Deneris. Respiratory Physiology & Neurobiology 159 (2007) 85–101. IFC tested in mouse.
Regionalization of the shark hindbrain: a survey of anancestral organization Isabel Rodríguez-Moldes, Ivan Carrer1, Sol Pose-Ménde1, Idoia Quintana-Urzainqu1, Eva Canda1, Ramón Anadó1, Sylvie Maza2 and Susana Ferreiro-Galve, Frontiers in Neuroanatomy, 2011, Spotted dogfish
Branch architecture of the fly larval abdominal serotonergic neurons, C. De La Roza and F. Reinoso-Suárez, Neuroscience, 8, 1-11, 2009, cat
The duration of Fgf8 isthmic organizer expression is key to patterning different tectal-isthmo-cerebellum structures, Tatsuya Sato and Alexandra L. Joyner, Development, 136, 3617-3626, 2009, mice
Gata2 is a tissue-specific post-mitotic selector gene for midbrain GABAergic neurons, Kaia Kala, Maarja Kersti Jordi Wolfgang Wurst, Marjo Salminen and Juha Partanen, Development, 136, 253-262, 2009, mice
Homeodomain transcription factor NKX2.2 functions in immature cells to control enteroendocrine differentiation and is expressed in gastrointestinal tumors, Yu-Cheng Wang, Emerick Gallego-Arteche, Gioia Iezza, Xiaochen Yuan, Mary R Matli, Su-PinChoo, Marlene B Zuraek, Ravi Gogia,Francis C Lynn, Michael S German, Emily K Bergsland, David B Donner, Robert S Warren, and Eric K Nakakura, Endocrine-Related Cancer, 16, 267–27, 2009, mice
Morphological and Physiological Characteristics of the Serotonin-Immunoreactive Neuron in the Antennal Lobe of the Male Oriental TobaccoBudworm, Helicoverpa assulta, Xin Cheng Zhao and Bente Gunnveig Berg, Chem. Senses 34: 363–372, 2009, moth.
Brain architecture in the terrestrial hermit crab Coenobita clypeatus(Anomura, Coenobitidae), a crustacean with a good aerial sense of smell Steffen Harzsch and Bill S Hansson, BMC Neuroscience 2008, 9:58, Hermit Crab.
Product Reviews
04/27/2012 - 11:59:08 AM
Phenomenal results
Generally we will perfuse, dissect and fix the tissue for 30min to 1 hour in 4% Paraformaldehyde. After cryopreservation in a 30% sucrose solution (1xPBS), the tissue is frozen in embedding media, cryosectioned, and mounted on microscope slides ready to be stained. Our immunohistochemistry protocol for this antibody is as follows:
Day 1
1. Post-fix sections in cold Acetone(-20°C) for 10 min at -20°C.
2. Wash slides 3X5 min. in 0.05M Tris Buffered Saline (TBS) pH7.4 .
3. Incubate sections with blocking solution (5% fishskin gelatin, 0.05% Triton X-100 at RT, 0.025 Sodium Azide)for 1 hour at room temperature.
4. Wash slides 2X5 min. in TBS.
5. Incubate in 1:1000 Rb/5HT overnight at 4°C.
Day 2
1. Wash slides with TBS 3X5min.
2. Incubate slides with secondary antiserum diluted in blocking buffer for 1 hour at room temp (i.e. donkey anti rabbit-Alexa 555 1:1000, Invitrogen).
3. Wash slides 2X5min. in TBS.
4. Wash slides 2X5min in ddWater.
5. Coverslip with Vectashield and view or store at 4°C.
--
Edgardo J. Arroyo, Ph. D.
VA CT HealthCare System/ Yale University
950 Campbell Ave. Bldg 34
Rm. 125
West Haven, CT 06516
T 203-932-5711
F 203-937-3801
M 267-259-0252
04/26/2012 - 10:07:12 AM
Superb Results
Tissue preparation:
The mice are perfused transcardially with 4% paraformaldehyde in 0.1M sodium phosphate buffer (PBS), pH 7.4 and the head of the mouse is blocked to remove the brainstem in situ. The tissue is post-fixed overnight at 4°C in 4% paraformaldehyde, then placed in 30% sucrose/0.1M PBS at 4°C until they sink. The tissue is then transferred to a 1:1 mix of 30% sucrose/Tissue Tek for 24 hours (at 4°C) before being embedded in Tissue Tek and frozen over dry ice. Frozen tissue blocks are sectioned at 20 µM, thaw-mounted onto glass slides, and stored at -20°C until use.
Indirect Immunofluorescence staining protocol:
Day 1
1. Remove slides from -20°C freezer and equilibrate to room temperature
2. Immerse slides in 1x PBST (890 ml of H2O + 100 ml of 10x PBS + 10 ml of 10% Triton X-100) for 15 min
3. Remove slides from PBST and expose tissue sections to blocking solution (1x PBST + 2% sheep serum) for 1 hr at room temperature
4. Expose tissue sections to primary antiserum (rabbit anti-5HT, ImmunoStar #20080) overnight @ 4°C. Primary antibody is stored at a 1:10 dilution and used at 1:8,000
Day 2
5. Wick off antiserum and wash tissue sections 3 x 10 min with 1xPBST
6. Incubate tissue sections with secondary antibody (e.g. goat anti-rabbit-FITC, Jackson ImmunoResearch Laboratories, Inc., diluted 1:200 in PBST) for 2 hrs in the dark at room temperature. After incubation with secondary antibody, wash tissue sections 3 x 10 min with 1x PBST in the dark
7. Treat tissue sections with freshly made p-phenylenediamine (1 mg/ml) for 1 min, then wash 2 x 10 min in 1x PBS.
8. Coverslip with glycerol gel and store at -20°C
Jeffery T. Erickson, PhD
Biology Department
The College of New Jersey
Ewing, NJ 08628
