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5-HT (Serotonin) Goat Antibody

5-HT (Serotonin) Goat Antibody
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Price: $275.00
Product ID : 20079
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Description

The ImmunoStar serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/400 - 1/800 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/5000 - 1/10,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique.

Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg of serotonin/BSA conjugate.

Host
: Goat

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum


Reacts With: Hamster, Pig, Monkey, Mouse, Mosquito, Pig Rat

Availability: In Stock

 

Download Product Spec Sheet:  5-HT (Serotonin) Goat Antibody 

Journal References:

Raphé neurons stimulate respiratory circuit activity by multiple mechanisms via endogenously released serotonin and substance P, Krzysztof Ptak, Tadashi Yamanishi1, Jason Aungst, Lorin S. Milescu, Ruli Zhang, George B Richerson, and Jeffrey C. Smith, J Neurosci., 29(12), 3720–3737, 2009, rat

Experimental evidence that the serotonin transporter mediates serotonin accumulation in LSO neurons of the postnatal mouse, Ann M. Thompsona, Glenn C. Thompson, Brain Research, 1253, 60–68, 2009, mice

Observation of fetal brain in a rat valproate-induced autism model: a developmental neurotoxicity study, Makiko Kuwagata, Tetsuo Ogawa, Seiji Shioda , Tomoko Nagata, Int. J. Devl Neuroscience, 1.006, 399-405, 2009, rat

Prospective identification of a multi-lineage progenitor in murine stomach epithelium, Xiaotan T. Qiao, Joshua W. Ziel, Wendy McKimpson, Blair B. Madison, Andrea Todisco, Juanita L. Merchant, Linda C. Samuelson, and Deborah L. Gumucio, Gastroenterology, 133(6, 1989–1998, 2007, mouse

Roles of ES Cell-Derived Gliogenic Neural Stem/Progenitor Cells in Functional Recovery after Spinal Cord Injury Gentaro Kumagai, Yohei Okada, Junichi Yamane, Narihito Nagoshi, Kazuya Kitamura, Masahiko Mukaino, Osahiko Tsuji, Kanehiro Fujiyoshi, Hiroyuki Katoh, Seiji Okada, Shinsuke Shibata, Yumi Matsuzaki, Satoshi Toh, Yoshiaki Toyama, Masaya Nakamura, Hideyuki Okano, PLoS ONE, November 2009 | Volume 4 | Issue 11 | e7706, mouse.

SIRT1 Regulation of Wakefulness and Senescence Senescence-Like, Phenotype in Wake Neurons Lori Panossian, Polina Fenik, Yan Zhu, Guanxia Zhan, Michael W. McBurney, and Sigrid Veasey, Center for Sleep and Respiratory Neurobiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, and 3Center for Cancer Therapeutics, Ottawa Hospital Research Institute, Department of Medicine and 4Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5, The Journal of Neuroscience, March 16, 2011 • 31(11):4025– 4036 • 4025, mouse.

Comparison of immunoreactivity to serotonin, FMRFamide and SCPb in the gut and visceral nervous system of larvae, pupae and adults of the yellow fever mosquito Aedes aegypti, Moffett SB and Moffett ADF, Journal of Insect Science, 5(20), 12pp, 2005.

Essential role for the Prader-Willi syndrome protein necdin in axonal outgrowth, Lee, Walker, Karten, Kuny, Tennese, O'Neil, Wevrick , Human Molecular Genetics, 14(5), 627-637, 2005.

Receptor Expression, Songyan Wang, Jianfeng Liu, Lin Li, and Burton M. Wice, Journal of Histochemistry & Cytochemistry, 52(1), 52(1), 2004

Serotonergic Retinopetal Axons in the Monkey Retina, Gastinger, Bordt, Bernal, Marshak , Current Eye Research, 30(12), 1089-1095, 2005.

Vasopressin/Serotonin Interactions in the Anterior Hypothalamus Control Aggressive Behavior in Golden Hamsters, Ferris CF, Melloni RH Jr, Koppel G, The Journal of Neuroscience, 17(11), 4331-4340, 1997.

Conditional Rhythmicity of Ventral Spinal Interneurons Defined by Expression of the Hb9 Homeodomain Protein , Wilson, Hartley, Maxwell, Todd, Lieberam, Kaltschmidt, Yoshida, Jessell, and Brownstone, The Journal of Neuroscience, 25(24), 5710-5719, 2005.

Individual Subtypes of Enteroendocrine Cells in the Mouse Small Intestine Exhibit Unique Patterns of Inositol 1,4,5-trisphosphate Receptor Expression, Songyan Wang, Jianfeng Liu, Lin Li, and Burton M. Wice, Journal of Histochemistry & Cytochemistry, 52(1), 52(1), 2004.

Serotonergic Retinopetal Axons in the Monkey Retina, Gastinger, Bordt, Bernal, Marshak , Current Eye Research, 30(12), 1089-1095, 2005.

s-Opioid Receptor Immunoreactivity: Distribution in Brainstem and Spinal Cord, and Relationship to Biogenic Amines and Enkephaline , Ulf Arvidsson, Robert J. Dado, Maureen Riedl, Jang-Hen Lee, Ping Y. Law, Horace H. Loh, Robert Elde, and Martin W. Wessendorf, The Journal of Neuroscience, 15(2), 1215-1235, 1995.

Product Reviews

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Reviewed by ggehrke@med.umich.edu
03/16/2012 - 05:51:57 AM
Great staining with Serotonin antibody
I utilized the antibody in formalin-fixed, free-floating mouse brain tissue at a concentration of 1:1000. For secondary detection I utilized Invitrogen Alexa-Fluor-conjugated secondary antibodies at 1:200.

The 5HT antibody always yielded great staining and we were very pleased with it. Indeed, we utilized this antibody for studies of dorsal raphe 5HT neurons as reported in our recent publication in Cell Metabolism (Lam, Leinnintger et al 2011, Cell Metab 13(5): 584-91).


Gina M. Leinninger, PhD
Research Investigator
Martin G. Myers Jr. Laboratory
University of Michigan