5-HT (Serotonin) 7 Receptor Antibody

The ImmunoStar 5-HT7 receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/300 in PBS - biotin/avidin-HRP Technique. Note: use of Triton X-100 or other detergents is not recommended.
The antibody was characterized by immunohistochemistry. Immunohistochemical staining of rat brain correlates well with Northern blot analysis, in situ hybridization and receptor autoradiography studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT7 receptor (8-23). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat 5-HT7A, 5-HT7B and 5-HT7C. There is also significant sequence overlap with the mouse and human forms.

Host: Rabbit

Quantity/Volume: 100 µL

State: Liquid

Reacts With: Rabbit, Rat

Predicted to React With: Hamster, Mouse

Download Product Spec. Sheet:  5-HT (Serotonin) 7 Receptor Antibody

Journal References:

1. Characterization of serotonergic receptors in rabbit, porcine and human conjunctivae, Helen Turner, Lawrence J. Alvarez, Oscar A. Candia, and Audrey M. Bernstein, Current Eye Research, 27(4), p. 205-215, 2003.  Tested in rabbit, pig, human.

2. IMMUNOCYTOCHEMICAL DETECTION OF THE SEROTONIN TRANSPORTER IN RAT BRAIN, C. SUR, H. BETZ and P. SCHLOSS, Neuroscience, 73, 217-231, 1996, Tested in rat.

3. Localization of 5-HT7 receptors in rat brain by immunocytochemistry, in situ hybridization, and agonist stimulated cFos expression, J.F. Neumaier, T.J. Sexton, J. Yracheta, A.M. Diaz, and M. Brownfield, Journal of Chemical Neuroanatomy, 21, 63-73, 2001, Tested in rat.

4. Pre- and Postsynaptic Localization of the 5-HT7 Receptor in Rat Dorsal Spinal Cord: Immunocytochemical Evidence, Stephane Doly, Jacqueline Fischer, Marie-Jeanne Brisorgueil, Daniel Verge, and Marie Conrath, The Journal of Comparative Neurology, 490, 256–269 , 2005, Tested in rat.