5-HT (Serotonin) 7 Receptor Antibody

The ImmunoStar 5-HT7 receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/300 in PBS - biotin/avidin-HRP Technique. Note: use of Triton X-100 or other detergents is not recommended.
The antibody was characterized by immunohistochemistry. Immunohistochemical staining of rat brain correlates well with Northern blot analysis, in situ hybridization and receptor autoradiography studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT7 receptor (8-23). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat 5-HT7A, 5-HT7B and 5-HT7C. There is also significant sequence overlap with the mouse and human forms.

Host: Rabbit

Quantity/Volume: 100 µL

State: Liquid

Reacts With: Rabbit, Rat

Availability:  In stock

Predicted to React With: Hamster, Mouse

Alternate Names:  5-hydroxytryptamine receptor 7; 5-HT-X,GPRFO; 5Ht7; 5-hydroxytryptamine (serotonin) receptor 7

Gene Symbol / ID, Accession #:  Htr7,65032

Download Product Spec. Sheet:  5-HT (Serotonin) 7 Receptor Antibody

Journal References:

Pre- and Postsynaptic Localization of the 5-HT7 Receptor in Rat Dorsal Spinal Cord: Immunocytochemical Evidence; Stephane Doly, Jacqueline Fischer, Marie-Jeanne Brisorgueil, Daniel Verge, and Marie Conrath; The Journal of Comparative Neurology,490:256–269 , 2005, Tested in Rat, Cites Diasorin AKA ImmunoStar, PubMed ID: 16082681

Characterization of serotonergic receptors in rabbit, porcine and human conjunctivae.; Helen Turner, Lawrence J. Alvarez, Oscar A. Candia, and Audrey M. Bernstein; Current Eye Research,27(4):205-215, 2003, Tested in Rabbit, Western Blot, IFC, PubMed ID: 14562171

Pronociceptive role of peripheral and spinal 5-HT7 receptors in the formalin test; He´ctor. Rocha-Gonza´leza, Alfredo Menesesa, Susan M. Carltonb, Vinicio Granados-Sotoa; International Association for the Study of Pain,117:182–192, 2005, Tested in Rat, PubMed ID: 16098671

Characteristics of the actions by which 5-HT affects electrical and mechanical activities in rabbit jugular vein; Takeo Itoh, Junko Kajikuri; Br J Pharmacol,164(3):979–991, 2011, Tested in Rabbit, PubMed ID: 21449974