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5-HT (Serotonin) 7 Receptor Antibody

5-HT (Serotonin) 7 Receptor Antibody
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Price: $375.00
Product ID : 24430
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Description

The ImmunoStar 5-HT7 receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/100 - 1/300 in PBS - biotin/avidin-HRP Technique. Note: use of Triton X-100 or other detergents is not recommended.
The antibody was characterized by immunohistochemistry. Immunohistochemical staining of rat brain correlates well with Northern blot analysis, in situ hybridization and receptor autoradiography studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT7 receptor (8-23). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat 5-HT7A, 5-HT7B and 5-HT7C. There is also significant sequence overlap with the mouse and human forms.

Photo Description: IHC image of neurons staining for the 5-HT7 receptor in the rat brain cortex. The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:500 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Host: Rabbit

Quantity/Volume: 100 µL

State: Liquid

Reacts With: Rabbit, Rat

Availability:  In stock

Alternate Names:  5-hydroxytryptamine receptor 7; 5-HT-X,GPRFO; 5Ht7; 5-hydroxytryptamine (serotonin) receptor 7

Gene Symbol / ID, Accession #:  Htr7,65032

 

Download Product Spec. Sheet 5-HT (Serotonin) 7 Receptor Antibody

 

Journal References:


These publications reference ImmunoStar's antibody product line, formerly sold by Diasorin, Incstar, and ImmunoNuclear.

Characterization of serotonergic receptors in rabbit, porcine and human conjunctivae.; Helen Turner, Lawrence J. Alvarez, Oscar A. Candia, and Audrey M. Bernstein; Current Eye Research,27(4):205-215, 2003, Tested in Rabbit, Western Blot, IFC, PubMed ID: 14562171

Pronociceptive role of peripheral and spinal 5-HT7 receptors in the formalin test; He´ctor. Rocha-Gonza´leza, Alfredo Menesesa, Susan M. Carltonb, Vinicio Granados-Sotoa; International Association for the Study of Pain,117:182–192, 2005, Tested in Rat, PubMed ID: 16098671

Maekawa, Takashi, et al. "Characteristics of the actions by which 5‐hydroxytryptamine affects electrical and mechanical activities in rabbit jugular vein graft." British journal of pharmacology 166.4 (2012): 1419-1432. Maekawa T, Komori K, Kajikuri J, Itoh T., Tested in Rabbit, IF method, PubMed ID: 22251164

Itoh, Takeo, and Junko Kajikuri. "Characteristics of the actions by which 5‐HT affects electrical and mechanical activities in rabbit jugular vein." British journal of Pharmacology 164.3 (2011): 979-991. Takeo Itoh, Junko Kajikuri, Tested in Rabbit, IF method, PubMed ID: 21449974

Khalmuratova, Roza, et al. "Immunohistochemical and biomolecular identification of 5-HT _ {7} receptor in rat vestibular nuclei." Journal of Vestibular Research 20.6 (2010): 401-406. Khalmuratova R, Hah YS, Ahn SK, Jeon SY, Kim JP, Balaban CD. Tested in Rat, IHC method, PubMed ID: 21248402

Brenchat, Alex, et al. "Pharmacological activation of 5-HT 7 receptors reduces nerve injury-induced mechanical and thermal hypersensitivity." Pain 149.3 (2010): 483-494. IHC method, PubMed ID: 20399562

Noga, Brian R., et al. "Locomotor-activated neurons of the cat. I. Serotonergic innervation and co-localization of 5-HT7, 5-HT2A, and 5-HT1A receptors in the thoraco-lumbar spinal cord." Journal of neurophysiology 102.3 (2009): 1560. Noga BR, Johnson DM, Riesgo MI, Pinzon A., Tested in Cat, ICC method, PubMed ID: 19571190

Iwasato, Takuji, et al. "Cortical adenylyl cyclase 1 is required for thalamocortical synapse maturation and aspects of layer IV barrel development." The Journal of Neuroscience 28.23 (2008): 5931-5943. Iwasato T, Inan M, Kanki H, Erzurumlu RS, Itohara S, Crair MC., Tested in Mouse, IF method, PubMed ID: 18524897

Parga, J., et al. "Serotonin decreases generation of dopaminergic neurons from mesencephalic precursors via serotonin type 7 and type 4 receptors." Developmental neurobiology 67.1 (2007): 10-22. Parga J, Rodriguez-Pallares J, Muñoz A, Guerra MJ, Labandeira-Garcia JL. Tested in Rat, IHC method, PubMed ID: 17443768

Doly, Stéphane, et al. "Pre‐and postsynaptic localization of the 5‐HT7 receptor in rat dorsal spinal cord: Immunocytochemical evidence." Journal of Comparative Neurology 490.3 (2005): 256-269. Doly S, Fischer J, Brisorgueil MJ, Vergé D, Conrath M., Tested in Rat, WB method, PubMed ID: 16082681

Muneoka, Katsumasa T., and Morikuni Takigawa. "5-Hydroxytryptamine 7(5-HT 7) receptor immunoreactivity-positive ‘stigmoid body’-like structure in developing rat brains." International journal of developmental neuroscience 21.3 (2003): 133-143. Tested in Rat, PubMed ID: 12711351

Belenky, Michael A., and Gary E. Pickard. "Subcellular distribution of 5‐HT1b and 5‐HT7 receptors in the mouse suprachiasmatic nucleus." Journal of Comparative Neurology 432.3 (2001): 371-388. Belenky MA, Pickard GE., IHC method, PubMed ID: 11246214

Contesse, Vincent, et al. "Pharmacological and molecular characterization of 5-hydroxytryptamine7 receptors in the rat adrenal gland." Molecular pharmacology 56.3 (1999): 552-561. Contesse V, Lenglet S, Grumolato L, Anouar Y, Lihrmann I, Lefebvre H, Delarue C, Vaudry H., Tested in Rat, PubMed ID: 10462543

Condron, Barry G., and Kai Zinn. "Regulated neurite tension as a mechanism for determination of neuronal arbor geometries in vivo." Current Biology 7.10 (1997): 813-816. Tested in Grasshopper, PubMed ID: 9368767


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