5-HT (Serotonin) 2C Receptor Antibody

he ImmunoStar 5-HT2C receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat choroid plexus and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are /500 - 1/1000 in PBS - Bn/Av-HRP technique. Intensification methods such as nickel will approximately double the dilution factor as recommended.

The antibody was characterized by immunohistochemistry and Western blot. Western blotting revealed a single band of approximately 70 kD. Due to the difficulty in running westerns with this antibody, ImmunoStar does not support or warrant the western blot application.  The western information is included as specificity information only.

Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT2C receptors.

Host: Rabbit

Quantity / Volume: 100 µL

State: Liquid

Reacts With: Mouse, Rat

Predicted to React With: Bovine, Human, Monkey, Pig, Rabbit

Alternate Names:  5-hydroxytryptamine receptor 2C; 5-HT2C; 5HT-1C; 5-HTR2C; 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled

Gene Symbol / ID, Accession #:  Htr2c,25187

Download Product Spec. Sheet:  5-HT (Serotonin) 2C Receptor Antibody

Journal References:

Activity modes in thalamocortical relay neurons are modulated by Gq/G11 family G-proteins – serotonergic and glutamatergic signaling; PhilippeCoulon,TatyanaKanyshkova,Tilman Broicher, Thomas Munsch, Nina  Wettschureck, Thomas Seidenbecher, Sven G. Meuth, Stefan Offermanns, Hans-Christian Pape and Thomas Budde; Cellular Neuroscience,4:132, 2010, Tested in Mouse, PubMed ID: 21267426

Complex Seizure Disorder Caused by Brunol4 Deficiency in Mice; Yan Yang, Connie L. Mahaffey, Nathalie Be Rube, Terry P. Maddatu, Gregory A. Cox, Wayne N. Frankel; PLoS Genet 3(7): e124. doi:10.,3:1275-1283, 2007, Tested in Mouse, Western Blot, PubMed ID: 17677002