5-HT (Serotonin) 2C Receptor Antibody

5-HT (Serotonin) 2C Receptor Antibody
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Price: $375.00
Product ID : 24505
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The ImmunoStar 5-HT2C receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat choroid plexus and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are /500 - 1/1000 in PBS - Bn/Av-HRP technique. Intensification methods such as nickel will approximately double the dilution factor as recommended.

The antibody was characterized by immunohistochemistry and Western blot. Western blotting revealed a single band of approximately 70 kD. Due to the difficulty in running westerns with this antibody, ImmunoStar does not support or warrant the western blot application.  The western information is included as specificity information only.

Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT2C receptors.

Photo Description:
Gray scale IHC image of coronal rat brain section staining for the 5-HT2C receptor (above) and high magnification image of receptor expression in the cortex of the rat brain (below). The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:1000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

5HT2C cortex #24505

Host: Rabbit

Quantity / Volume: 100 µL

State: Liquid

Reacts With: Mouse, Rat

Alternate Names:  5-hydroxytryptamine receptor 2C; 5-HT2C; 5HT-1C; 5-HTR2C; 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled

Gene Symbol / ID, Accession #:  Htr2c,25187


Download Product Spec. Sheet5-HT (Serotonin) 2C Receptor Antibody


Journal References:

These publications reference ImmunoStar's antibody product line, formerly sold by Diasorin, Incstar, and ImmunoNuclear.

Zhang, Yu, et al. "Involvement of spinal serotonin receptors in electroacupuncture anti-hyperalgesia in an inflammatory pain rat model." Neurochemical research 36.10 (2011): 1785-1792. Zhang Y, Li A, Xin J, Lao L, Ren K, Berman BM, Tan M, Zhang RX. Tested in Rat, PubMed ID: 21556842

Cellular Neuroscience, 4:132. (2010) Activity modes in thalamocortical relay neurons are modulated by Gq/G11 family G-proteins – serotonergic and glutamatergic signaling, Philippe Coulon,Tatyana Kanyshkova,Tilman Broicher, Thomas Munsch, Nina  Wettschureck, Thomas Seidenbecher, Sven G. Meuth, Stefan Offermanns, Hans-Christian Pape and Thomas Budde, Tested in Mouse, IF method, PubMed ID: 21267426

PLoS Genet 3(7): e124. doi:10.,3:1275-1283. (2007) Complex Seizure Disorder Caused by Brunol4 Deficiency in Mice. Yan Yang, Connie L. Mahaffey, Nathalie Be Rube, Terry P. Maddatu, Gregory A. Cox, Wayne N. Frankel, Tested in Mouse, WB method, PubMed ID: 17677002

Holmes, Gregory M. "5-Hydroxytryptamine 2C receptors on pudendal motoneurons innervating the external anal sphincter." Brain research 1057.1 (2005): 65-71. Tested in Rat, PubMed ID: 16125683

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