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5-HT (Serotonin) 2A Receptor Antibody

24288
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  • Refer to Dr. Schwendt's review
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Price: $375.00
Product ID : 24288
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Description

The antibody is provided as 100 uL of affinity purified serum in PBS (0.02 M sodium phosphate with 0.15 M sodium chloride, pH 7.5) with 1% BSA (bovine serum albumin), and 0.02% sodium azide.  Reacts with mouse, rat, and rabbit species.

The images above labeled "ImmunoStar Rabbit 5-HT 2A receptor antibody" are the results of staining of the mouse 5-HT 2A receptor in the brain, courtesy of Dr. Magdalena Zaniewska, Max-Delbruck-Centrum fur Molekulare Medizin Berlin, Germany.

The ImmunoStar 5HT 2A receptor antibody was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex, amygdala and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/300 - 1/500 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique . The addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solution will approximately double the dilution factor as recommended.

Immunolabeling is completely abolished by preadsorption with synthetic rat 5HT2A receptor (22-41). Immunolabeling of Western blot revealed a single band of approximately 53kD. Due to the difficulty in running westerns with this antibody, ImmunoStar does not support or warrant the western blot application.  The western information is included as specificity information only.

 

Host: Rabbit

Quantity / Volume: 100 uL

State: Liquid

Reacts With: Marine, Mouse, Rabbit, Rat

Alternate Names:  5-hydroxytryptamine receptor 2A; 5Ht-2; 5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled

Gene Symbol / ID, Accession #:  Htr2a,29595

 

Download Product Spec Sheet: 5-HT (Serotonin) 2A Receptor Antibody

 

Journal References:


Pre- and Postsynaptic Localization of the 5-HT7 Receptor in Rat Dorsal Spinal Cord: Immunocytochemical Evidence; Stephane Doly, Jacqueline Fischer, Marie-Jeanne Brisorgueil, Daniel Verge, and Marie Conrath; The Journal of Comparative Neurology,490:256–269 , 2005, Tested in Rat, Cites Diasorin AKA ImmunoStar, PubMed ID: 16082681

Serotonin 5-HT2A and 5-HT5A receptors are expressed by different motoneuron populations in rat Onuf's nucleus; Xu C, Giuliano F, Sun XQ, Brisorgueil MJ, Leclerc P, Vergé D, Conrath M; J Comp Neurol.,502(4):620-34, 2007, Tested in Rat, Cites Diasorin AKA ImmunoStar, PubMed ID: 17394137

The 5-HT2A Receptor Is Widely Distributed in the Rat Spinal Cord and Mainly Localized at the Plasma Membrane of Postsynaptic Neurons; Stephane Doly, Alexandra Madeira, Jacqueline Fischer, Marie-Jeanne Brisorgueil, Genevieve Daval, Rozenn Bernard, Daniel Verge, and Marie Conrath; The Journal of Comparative Neurology,472:496–511 , 2004, Tested in Rat, IFC, Cites Diasorin AKA ImmunoStar, PubMed ID: 15065122

5-HT2a receptors in the rabbit retina: Potential presynaptic modulators.; Pootanakit K, Prior KJ, Hunter DD, Brunken WJ; Visual Neurosci,16:221-230, 1999, Tested in Rabbit, Cites Incstar AKA ImmunoStar, PubMed ID: 10367957

Enhancement of mesenteric artery contraction to 5-HT depends on Rho kinase ; Takayuki Matsumoto, TsuneoKobayashi, Keiko Ishida, Kumiko Taguchi and Katsuo Kamata; Hoshi University,1412:RP. 1-6, 2009, Tested in Mouse, Western Blot, PubMed ID: 20590603

The pre-nervous serotonergic system of developing sea urchin embryos and larvae: pharmacologic and immunocytochemical evidence; Gennady A. Buznikov,  Robert E. Lyudmila A. Nikitina, Vladimir V. , and Jean M. Lauder; Neurochemical Research,30:825–837, 2005, Tested in Marine, PubMed ID: 16187217

The 5-Hydroxytryptamine2A Receptor Is Involved in (+)-Norfenfluramine-Induced Arterial contraction and blood pressure increase in deoxycorticosterone acetate-salt hypertension ; Wei Ni, Gregory D. Fink, and Stephanie W. Watts; Journal Of Pharmacology And Experimental Therapeutics,321:485-491, 2007, Tested in Rat, PubMed ID: 17289838

Up-regulation of 5-HT2 receptors is involved in the increased Hreflex amplitude after contusive spinal cord injury; Jae K. Lee, Christopher S. Johnson, and Jean R. Wrathall; Exp Neurol,203:502-511, 2007, Tested in Rat, PubMed ID: 17059818

Activity modes in thalamocortical relay neurons are modulated by Gq/G11 family G-proteins – serotonergic and glutamatergic signaling; PhilippeCoulon,TatyanaKanyshkova,Tilman Broicher, Thomas Munsch, Nina  Wettschureck, Thomas Seidenbecher, Sven G. Meuth, Stefan Offermanns, Hans-Christian Pape and Thomas Budde; Cellular Neuroscience,4:132, 2010, Tested in Mouse, PubMed ID: 21267426

Chronic treatment of hydroxytryptamine type 2a receptor ; Akio Kodama, MD, PhD, Kimihiro Komori, MD, PhD, Junko Kajikuri, PhD, and Takeo Itoh, PhD; Journal Of Vascular Surgery,,50, 3:617-625, 2009, Tested in Rabbit, PubMed ID: 19700096

Characteristics of the actions by which 5-HT affects electrical and mechanical activities in rabbit jugular vein; Takeo Itoh, Junko Kajikuri; Br J Pharmacol,164(3):979–991, 2011, Tested in Rabbit, PubMed ID: 21449974

Stress impairs 5-HT2A receptor-mediated serotonergic facilitation of GABA release in juvenile rat basolateral amygdala; Xiaolong Jiang, Guoqiang Xing, Chunhui Yang, Ajay Verma, Lei Zhang and He Li; Neuropsychopharmacology,34:410-423, 2009, Tested in Rat, PubMed ID: 18536707

Robust upregulation of serotonin 2A receptors after chronic spinal transection of rats: an immunohistochemical study.; Xiang-Yu Konga,b, Jacob Wieneckea,c, Hans Hultborna, Mengliang Zhanga; Brain Research,1320:60-68, 2010, Tested in Rat, PubMed ID: 20085755

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Product Reviews

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Reviewed by user
03/11/2014 - 10:36:22 AM
Only your AB shows a specific signal
(Please refer to the above western blot image submitted by Dr. Schwendt.)

We have been interested in detecting changes of 5-HT2a in rodent brain, primarily by immunoblotting, also by immunohistochemistry. We have tested several commercial antibodies, with only your Ab showing a specific signal, as verified by brain distribution, preadsorbtion and by a lack of signal in KO animals.

We have optimized a immunoblotting protocol which works well to detect 5-HT2a in rat brain lysates. Mouse tissue is a little bit more difficult but still possible

Immunoblotting: Rat or mouse brain samples were lysed by brief sonication in a buffer containing: 1% SDS in 1xPBS, protease inhibitors (Complete Mini, Roche). Samples were mixed with a modified Leamli buffer (containing a reducing agent; 40mM DTT), incubated at 37C for 30mins and separated by SDS-PAGE. Separated proteins were then blotted onto a PVDF membrane, blocked with 5% milk/TBS/0.1% Tween80 and probed with anti-5HT2a antibody (1:500, Immunostar #24288) followed by anti-rabbit HRP- conjugated secondary antibody (1:5000, Jackson Immuno). Signal was visualized using ECL2 kit according to manufacturer's instructions (Thermo).

Image Samples: 1 (Rat prefrontal cortex, 25ug), 2 (Rat laterodorsal thalamus, 25ug), 3 (Wt mouse cortex, 25 ug), 4 (5-HT2a KO mouse cortex, 25 ug), 5 (Wt mouse cortex, 75 ug), 6 (5-HT2a KO mouse cortex, 75 ug). As expected, sample in lane 2 showed low expression, and samples in lanes 4 and 6 showed no expression of 5-HT2a receptors. Mouse tissues obtained from Dr. Gonzalez-Maeso (Mount Sinai Hospital, NY) indicates detected 5Ht2a receptor signal.


Submitted by:

Marek Schwendt, PhD
Psychology Department
University of Florida
Gainesville, Fl


Reviewed by user
11/15/2012 - 02:37:02 PM
Antibodies are specific for mouse receptor
[The two pictures above labeled "ImmunoStar's Rabbit 5-HT 2A receptor antibody" are courtesy of Dr. Magdalena Zaniewska.]

Here are the results of staining of the mouse 5-HT 2A receptors in the brain with the use of Immunostar's antibodies (Cat no: 24288). I think the antibodies are specific for the mouse receptor (no receptor labeling in the brain of 5-HT 2A receptor knockout mouse) and can be used in a Western blot. What I already found out is that 5-HT 2A receptors in the cortex seem to be glycosylated (band around 75 kDa) since incubation with PNGaseF releases the protein that is around 50 kDa.

We received the 5-HT 2A receptor knockout animals from Dr. Joelle Adrien from another research team in Paris (see below).


Reviewed by:

Dr. Magdalena Zaniewska
Molekularbiologie von Hormonen im Herz-Kreislaufssystem
Max-Delbrϋck-Centrum fϋr Molekulare Medizin
Berlin, Germany

Dr. Joelle Adrien
Directeur de Recherches a l'Inserm
Faculte de Medecine Pierre et Marie Curie - Site Pitie Salpetriere
Paris, France