5-HT (Serotonin) 1A Receptor Antibody

The histochemical antibody for 5-HT1A receptor is generated in a rabbit against synthetic peptide sequence corresponding to amino acids 294-312 of the rat 5-HT1A receptor. The antiserum is provided as 100 µl of affinity purified serum containing 1% BSA. Controls: The ImmunoStar 5-HT1A receptor antiserum was quality control tested using standard immunohistochemical methods.

The antiserum demonstrates strongly positive labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/200 - 1/300 in PBS - Bn/Av-HRP detection. Intensification methods such as nickel will approximately double the dilution factor as recommended.



The antibody was characterized by immunohistochemistry and Western blot. Western blot showed a single band of approximately 45 kD using a dilution of 1/100. Due to the difficulty in running westerns with this antibody, ImmunoStar does not support or warrant the western blot application.  The western information is included as specificity information only. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography.

BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT1A receptors.

Host: Rabbit

Quantity / Volume: 100 µL

State: Liquid

Availability:  In stock

Reacts With: Mouse, Rat 

Predicted to React With: Bovine, Human, Monkey, Pig, Rabbit, Squirrel 

Alternate Names:  5-hydroxytryptamine receptor 1A; ADRB2RL1; ADRBRL1; RAT5HT1A, 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled

Gene Symbol / ID, Accession #:  Htr1a, 24473

Download Spec. Sheet: 5-HT 1A Receptor Antibody

Journal References:

Bone Morphogenetic Protein Regulation of Enteric Neuronal Phenotypic Diversity: Relationship to Timing of Cell Cycle Exit; Alcmène Chalazonitis, Tuan.D. Pham, Zhishan Li, Daniel Roman, Udayan Guha, William Gomes, Lixin Kan, John A. Kessler, and Michael D. Gershon, Thomas Finger; J Comp Neurol.,209:474-492, 2008, Tested in Mouse and Rat, PubMed ID: 18537141