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5-HT (Serotonin) 1A Receptor Antibody

5-HT (Serotonin) 1A Receptor Antibody
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Price: $375.00
Product ID : 24504
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Description

The histochemical antibody for 5-HT1A receptor is generated in a rabbit against synthetic peptide sequence corresponding to amino acids 294-312 of the rat 5-HT1A receptor. The antiserum is provided as 100 µl of affinity purified serum containing 1% BSA. Controls: The ImmunoStar 5-HT1A receptor antiserum was quality control tested using standard immunohistochemical methods.

The antiserum demonstrates strongly positive labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/200 - 1/300 in PBS - Bn/Av-HRP detection. Intensification methods such as nickel will approximately double the dilution factor as recommended.

The antibody was characterized by immunohistochemistry and Western blot. Western blot showed a single band of approximately 45 kD using a dilution of 1/100. Due to the difficulty in running westerns with this antibody, ImmunoStar does not support or warrant the western blot application.  The western information is included as specificity information only. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography.

BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT1A receptors.

Photo Description: IHC image of neurons staining for the 5-HT1A receptor in the rat cortex (above) and 5HT1A receptor staining of astrocytes in the rat hippocampus (below). The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:500 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Hippocampus

Host: Rabbit

Quantity / Volume: 100 µL

State: Liquid

Availability:  In Stock

Reacts With: Guinea Pig, Human, Monkey, Mouse, Rat

Alternate Names:  5-hydroxytryptamine receptor 1A; ADRB2RL1; ADRBRL1; RAT5HT1A, 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled

Gene Symbol / ID, Accession #:  Htr1a, 24473

 

Download Spec. Sheet: 5-HT 1A Receptor Antibody

 

Journal References:


Vasudeva, Rani K., and Barry D. Waterhouse. "Cellular Profile of the Dorsal Raphe Lateral Wing Sub-region: Relationship to the Lateral Dorsal Tegmental Nucleus." Journal of chemical neuroanatomy (2014).

Wang, Guo-Du, et al. "Mast cell expression of the serotonin1A receptor in guinea pig and human intestine." American Journal of Physiology-Gastrointestinal and Liver Physiology 304.10 (2013): G855-G863. Tested in Human and Guinea Pig, PubMed ID: 23518679

Ahn, Seong-Ki, and Carey D. Balaban. "Distribution of 5-HT and 5-HT receptors in the inner ear." Brain research 1346 (2010): 92-101. Tested in Rat and Monkey, PubMed ID: 20510890

Hadjighassem, Mahmoud R., et al. "Human Freud-2/CC2D1B: a novel repressor of postsynaptic serotonin-1A receptor expression." Biological psychiatry 66.3 (2009): 214-222. PubMed ID: 19423080

Alcmène Chalazonitis, Tuan.D. Pham, Zhishan Li, Daniel Roman, Udayan Guha, William Gomes, Lixin Kan, John A. Kessler, and Michael D. Gershon, Thomas Finger; Bone Morphogenetic Protein Regulation of Enteric Neuronal Phenotypic Diversity: Relationship to Timing of Cell Cycle Exit; J Comp Neurol.,209:474-492, Tested in Mouse and Rat, PubMed ID: 18537141

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