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5-HIAA (5-Hydroxyindoleacetic Acid) Antibody

5-HIAA (5-Hydroxyindoleacetic Acid) Antibody
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Price: $250.00
Product ID : 24274
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Description

The antibody produces moderate labeling of raphe neurons in normal rat. In rats whose serotonergic system has been activated, staining intensity is increased to a maximum label. Recommended dilutions of the antiserum are 1/200-1/400 for indirect immunofluorescence and 1/4000-1/8000 for biotin-streptavidin/HRP technique.

The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Schipper and Tilders, 1983). Results showed that the 5-HIAA antibody dose dependently stained 5-HIAA but did not stain any concentration of 5-HT or 5-HTP. The antiserum was also tested by pre-adsorption at 25 µg/mL with various BSA conjugates. While pre-adsorption with 5-HIAA conjugate completely eliminates immunolabeling, pre-adsorption with conjugates of 5-HT,5-HTP and dopamine had no effect on staining intensity or distribution of stain.

Photo Description: IHC image of neurons staining for 5-H1AA in the raphe nucleus of the rat brainstem. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. 


Host: Rabbit

Quantity/Volume: 100 µL

State: Liquid

Reacts With: : Mollusc, Rat

Download Product Spec. Sheet: 5-HIAA Antibody

Journal References:


These publications reference ImmunoStar's antibody product line, formerly sold by Diasorin, Incstar, and ImmunoNuclear.

Andrey M. Mazarati, Roger A. Baldwin, Steve Shinmei and Raman Sankar; In vivo interaction between serotonin and galanin receptors types 1 and 2 in the dorsal raphe: implication for limbic seizures; Journal of Neurochemistry,95:1495–1503. Tested in Rat, PubMed ID: 16219029

Fickbohm, David J., Nadja Spitzer, and Paul S. Katz. "Pharmacological manipulation of serotonin levels in the nervous system of the opisthobranch mollusc Tritonia diomedea." The Biological Bulletin 209.1 (2005): 67-74. Tested in Tritonia Diomedea (Sea Slug), PubMed ID: 16110094

Appel, NATHAN M., JOSEPH F. Contrera, and ERROL B. De Souza. "Fenfluramine selectively and differentially decreases the density of serotonergic nerve terminals in rat brain: evidence from immunocytochemical studies." Journal of Pharmacology and Experimental Therapeutics 249.3 (1989): 928-943. Tested in Rat, PubMed ID: 2732954

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