Description
The ImmunoStar Mu Opioid Receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat caudate putamen and spinal cord (dorsal horn) using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/6000 – 1/10000 in PBS/0.3% Triton X-100 – Biotin/avidin-HRP Technique.
Preadsorption with MOR peptide (384-398) at 10 µg/ml completely eliminates labeling. The specificity of the antiserum was determined by immunolabeling of transfected cells and immunoisolation studies.
Photo Description: IHC image of mu opioid receptor staining in the rat striatum. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Host: Rabbit
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Cat, Chicken, Frog, Guinea Pig, Human, Monkey, Mouse, Rat, Starling, Tadpole
Availability: In Stock
Alternate Names: MOR-1; Mu-type opioid receptor; M-OR1; Mu opiate receptor; Opioid receptor B; MUOR1; M-OR-1; MORA; oprm; opioid receptor, mu 1, anti-MOR
Gene Symbol: Oprm1
RRID: AB_572251
Database Links:
Entrez Gene: 101084452 Cat
Entrez Gene: 421644 Chicken
Entrez Gene: 100487937 Frog
Entrez Gene: 100379267 Guinea Pig
Entrez Gene: 4988 Human
Entrez Gene: 574141 Monkey
Entrez Gene: 18390 Mouse
Entrez Gene: 25601 Rat
Entrez Gene: 106859038 Starling
Technical Sheets
This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.
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Reviews
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MOR antibody 24216
This is an excellent antibody to label MORs in the rat spinal cord using immunofluorescence. I have used it to study MOR internalization induced by opioid release in the following papers:
Chen W, Marvizon JC (2009) Acute inflammation induces segmental, bilateral, supraspinally mediated opioid release in the rat spinal cord, as measured by µ-opioid receptor internalization. Neuroscience 161:157-172.
Chen W, Song B, Lao L, Perez OA, Kim W, Marvizon JCG (2007) Comparing analgesia and µ-opioid receptor internalization produced by intrathecal enkephalin: Requirement for peptidase inhibition. Neuropharmacology 53:664-667.
Chen W, Song B, Marvizon JC (2008a) Inhibition of opioid release in the rat spinal cord by a2C adrenergic receptors. Neuropharmacology 54:944-953.
Chen W, Song B, Zhang G, Marvizon JC (2008b) Effects of veratridine and high potassium on µ-opioid receptor internalization in the rat spinal cord: stimulation of opioid release versus inhibition of internalization. J Neurosci Methods 170:285-293.
Marvizon JC, Grady EF, Waszak-McGee J, Mayer EA (1999) Internalization of m-opioid receptors in rat spinal cord slices. Neuroreport 10:2329-2334.
Song B, Chen W, Marvizon JC (2007) Inhibition of opioid release in the rat spinal cord by serotonin 5-HT1A receptors. Brain Res 1158:57-62.
Song B, Marvizon JC (2003a) Peptidases prevent m-opioid receptor internalization in dorsal horn neurons by endogenously released opioids. J Neurosci 23:1847-1858.
Song B, Marvizon JCG (2003b) Dorsal horn neurons firing at high frequency, but not primary afferents, release opioid peptides that produce m-opioid receptor internalization in the rat spinal cord. J Neurosci 23:9171-9184.
Song B, Marvizon JCG (2005) NMDA receptors and large conductance calcium-sensitive potassium channels inhibit the release of opioid peptides that induce m-opioid receptor internalization in the rat spinal cord. Neuroscience 136:549-562.
Walwyn W, Chen W, Kim H, Minasyan A, Ennes H, McRoberts JA, Marvizon JC (2016) Sustained suppression of hyperalgesia during latent sensitization by µ, δ and κ opioid receptors and α2A adrenergic receptors – role of constitutive activity. J Neurosci (in press).
I have tried other MOR antibodies but this is by far the best.